Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells
Abstract Background The expression profiles of the intestinal mucosa have not been comprehensively investigated in asthma. We aimed to explore this in the Correlated Expression and Disease Association Research (CEDAR) patient cohort. Methods Differential expression analysis of ileal, transverse colo...
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doaj-33c7a3601960439b953562cdc9fe71be2021-08-01T11:41:08ZengBMCAllergy, Asthma & Clinical Immunology1710-14922021-07-0117111110.1186/s13223-021-00584-9Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cellsJan K. Nowak0Marzena Dworacka1Nazgul Gubaj2Arystan Dossimov3Zhumabek Dossimov4Jarosław Walkowiak5Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical SciencesDepartment of Pharmacology, Poznan University of Medical SciencesDepartment of Pediatric Diseases No. 2, West Kazakhstan Marat Ospanov Medical UniversityDepartment of Pediatric Diseases No. 2, West Kazakhstan Marat Ospanov Medical UniversityDepartment of Pediatric Diseases No. 2, West Kazakhstan Marat Ospanov Medical UniversityDepartment of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical SciencesAbstract Background The expression profiles of the intestinal mucosa have not been comprehensively investigated in asthma. We aimed to explore this in the Correlated Expression and Disease Association Research (CEDAR) patient cohort. Methods Differential expression analysis of ileal, transverse colon, and rectal biopsies were supplemented by a comparison of transcriptomes from platelets and leukocytes subsets, including CD4+, CD8+, CD14+, CD15+, and CD19+ cells. Asthma patients (n = 15) and controls (n = 15) had similar age (p = 0.967), body mass index (p = 0.870), similar numbers of females (80%) and smoking rates (13.3%). Results Significant differential expression was found in the ileum alone, and not in any other cell/tissue types. More genes were found to be overexpressed (1,150) than under-expressed (380). The most overexpressed genes included Fc Fragment of IgG Binding Protein (FCGBP, logFC = 3.01, pFDR = 0.015), Mucin 2 (MUC2, logFC = 2.78, pFDR = 0.015), and Alpha 1B Defensin (DEFA1B, logFC = 2.73, pFDR = 0.024). Gene ontology implicated the immune system, including interleukins 4 and 13, as well as antimicrobial peptides in this overexpression. There was concordance of gene over- (STAT1, XBP1) and underexpression (NELF, RARA) in asthma and Crohn’s disease ileum when our results were compared to another dataset (p = 3.66 × 10–7). Conclusion Ileal mucosa in asthma exhibits a specific transcriptomic profile, which includes the overexpression of innate immune genes, mostly characteristic of Paneth and goblet cells, in addition to other changes that may resemble Crohn’s disease.https://doi.org/10.1186/s13223-021-00584-9CrohnInflammatory bowel diseaseMucinIleumAirway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan K. Nowak Marzena Dworacka Nazgul Gubaj Arystan Dossimov Zhumabek Dossimov Jarosław Walkowiak |
spellingShingle |
Jan K. Nowak Marzena Dworacka Nazgul Gubaj Arystan Dossimov Zhumabek Dossimov Jarosław Walkowiak Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells Allergy, Asthma & Clinical Immunology Crohn Inflammatory bowel disease Mucin Ileum Airway |
author_facet |
Jan K. Nowak Marzena Dworacka Nazgul Gubaj Arystan Dossimov Zhumabek Dossimov Jarosław Walkowiak |
author_sort |
Jan K. Nowak |
title |
Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells |
title_short |
Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells |
title_full |
Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells |
title_fullStr |
Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells |
title_full_unstemmed |
Expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of Paneth and goblet cells |
title_sort |
expression profiling of ileal mucosa in asthma reveals upregulation of innate immunity and genes characteristic of paneth and goblet cells |
publisher |
BMC |
series |
Allergy, Asthma & Clinical Immunology |
issn |
1710-1492 |
publishDate |
2021-07-01 |
description |
Abstract Background The expression profiles of the intestinal mucosa have not been comprehensively investigated in asthma. We aimed to explore this in the Correlated Expression and Disease Association Research (CEDAR) patient cohort. Methods Differential expression analysis of ileal, transverse colon, and rectal biopsies were supplemented by a comparison of transcriptomes from platelets and leukocytes subsets, including CD4+, CD8+, CD14+, CD15+, and CD19+ cells. Asthma patients (n = 15) and controls (n = 15) had similar age (p = 0.967), body mass index (p = 0.870), similar numbers of females (80%) and smoking rates (13.3%). Results Significant differential expression was found in the ileum alone, and not in any other cell/tissue types. More genes were found to be overexpressed (1,150) than under-expressed (380). The most overexpressed genes included Fc Fragment of IgG Binding Protein (FCGBP, logFC = 3.01, pFDR = 0.015), Mucin 2 (MUC2, logFC = 2.78, pFDR = 0.015), and Alpha 1B Defensin (DEFA1B, logFC = 2.73, pFDR = 0.024). Gene ontology implicated the immune system, including interleukins 4 and 13, as well as antimicrobial peptides in this overexpression. There was concordance of gene over- (STAT1, XBP1) and underexpression (NELF, RARA) in asthma and Crohn’s disease ileum when our results were compared to another dataset (p = 3.66 × 10–7). Conclusion Ileal mucosa in asthma exhibits a specific transcriptomic profile, which includes the overexpression of innate immune genes, mostly characteristic of Paneth and goblet cells, in addition to other changes that may resemble Crohn’s disease. |
topic |
Crohn Inflammatory bowel disease Mucin Ileum Airway |
url |
https://doi.org/10.1186/s13223-021-00584-9 |
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