Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene
It is fundamental for gene transformation and ecosystem hazard evaluation to study the pollen characteristics of transgenic plants. In this research, the characteristics of pollen from 7- or 8-year-old transgenic apple plants carrying an exogenous CpTI gene were analyzed. The results showed that the...
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KeAi Communications Co., Ltd.
2015-07-01
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doaj-33c83d3ded00429e9e171725cf0d9a402021-04-02T11:29:50ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412015-07-011131010.16420/j.issn.2095-9885.2015-0002Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI GeneShi Xiaoxin0Zhou Ruijin1Wang Qianqian2Chang Tengfei3Feng Shasha4Du Guoqiang5College of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaCollege of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaCollege of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaCollege of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaCollege of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaCollege of Horticulture, Agricultural University of Hebei, Baoding, Hebei 071001, ChinaIt is fundamental for gene transformation and ecosystem hazard evaluation to study the pollen characteristics of transgenic plants. In this research, the characteristics of pollen from 7- or 8-year-old transgenic apple plants carrying an exogenous CpTI gene were analyzed. The results showed that there was no significant difference in terms of size, morphology, or exine ornamentation between the pollen of the transgenic plants and the non-transgenic control. However, the transgenic plants had more abnormal pollen grains. Of the 13 transgenic lines tested, 12 had a significantly lower amount of pollen and six exhibited a significantly lower germination rate when cultured in vitro. The pollen viability of three transgenic lines was determined, with two showing significantly lower viability than the control. The transgenic Gala apple pollen grains germinated normally via controlled pollination on Fuji apple stigmas. However, the pollen tubes extended relatively slowly during the middle and late development stages, and another 8 h were needed to reach the ovules compared with the control. The gibberellic acid concentration in transgenic Gala apple flowers was lower than in the non-transgenic control during all development stages tested. The abscisic acid concentration in the transgenic flowers was lower during the pink stage, and higher during the ball and fully open stages. Microscopic observation of the anther structure showed no difference. The tapetum of the pollen sac wall in transgenic plants decomposed late and affected pollen grain development, which could be one of the reasons for the lower number of pollen grains and poor viability in the transgenic plants.http://www.sciencedirect.com/science/article/pii/S246801411501002Xappleexogenous genepollenviabilitytapetum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shi Xiaoxin Zhou Ruijin Wang Qianqian Chang Tengfei Feng Shasha Du Guoqiang |
spellingShingle |
Shi Xiaoxin Zhou Ruijin Wang Qianqian Chang Tengfei Feng Shasha Du Guoqiang Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene Horticultural Plant Journal apple exogenous gene pollen viability tapetum |
author_facet |
Shi Xiaoxin Zhou Ruijin Wang Qianqian Chang Tengfei Feng Shasha Du Guoqiang |
author_sort |
Shi Xiaoxin |
title |
Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene |
title_short |
Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene |
title_full |
Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene |
title_fullStr |
Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene |
title_full_unstemmed |
Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene |
title_sort |
characteristics of pollen from transgenic lines of apple carrying the exogenous cpti gene |
publisher |
KeAi Communications Co., Ltd. |
series |
Horticultural Plant Journal |
issn |
2468-0141 |
publishDate |
2015-07-01 |
description |
It is fundamental for gene transformation and ecosystem hazard evaluation to study the pollen characteristics of transgenic plants. In this research, the characteristics of pollen from 7- or 8-year-old transgenic apple plants carrying an exogenous CpTI gene were analyzed. The results showed that there was no significant difference in terms of size, morphology, or exine ornamentation between the pollen of the transgenic plants and the non-transgenic control. However, the transgenic plants had more abnormal pollen grains. Of the 13 transgenic lines tested, 12 had a significantly lower amount of pollen and six exhibited a significantly lower germination rate when cultured in vitro. The pollen viability of three transgenic lines was determined, with two showing significantly lower viability than the control. The transgenic Gala apple pollen grains germinated normally via controlled pollination on Fuji apple stigmas. However, the pollen tubes extended relatively slowly during the middle and late development stages, and another 8 h were needed to reach the ovules compared with the control. The gibberellic acid concentration in transgenic Gala apple flowers was lower than in the non-transgenic control during all development stages tested. The abscisic acid concentration in the transgenic flowers was lower during the pink stage, and higher during the ball and fully open stages. Microscopic observation of the anther structure showed no difference. The tapetum of the pollen sac wall in transgenic plants decomposed late and affected pollen grain development, which could be one of the reasons for the lower number of pollen grains and poor viability in the transgenic plants. |
topic |
apple exogenous gene pollen viability tapetum |
url |
http://www.sciencedirect.com/science/article/pii/S246801411501002X |
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