Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
Background. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sa...
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2012-01-01
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Series: | Infectious Diseases in Obstetrics and Gynecology |
Online Access: | http://dx.doi.org/10.1155/2012/872169 |
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doaj-33cc2c88472741eba5f037dbb8cc53212020-11-24T23:13:56ZengHindawi LimitedInfectious Diseases in Obstetrics and Gynecology1064-74491098-09972012-01-01201210.1155/2012/872169872169Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCRMahnaz Mahmoudi Rad0Ameneh Sh Zafarghandi1Maryam Amel Zabihi2Mahkam Tavallaee3Yasaman Mirdamadi4Skin Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, IranDepartment of Obstetrics and Gynecology, Mahdieh Educational Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, IranDepartment of Obstetrics and Gynecology, Mahdieh Educational Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, IranFaculty of Health Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, CanadaSkin Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, IranBackground. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified. The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed. Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings. Results. C. albicans and C. glabrata were the most common species isolated from the specimens. A mix of C. glabrata and C. albicans was the most common mixed infection isolated from the samples. The analysis revealed a significant positive association between older age and infection with C. glabrata isolates (Spearman’s rho = 0.89, P=0.015). Conclusion. Multiplex PCR is a fast, yet reliable method to identify Candida species. C. albicans and then C. glabrata are the two most common causes of vulvovaginal candidiasis. The number of mixed fungal infections is higher among Iranian population compared to international reports.http://dx.doi.org/10.1155/2012/872169 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahnaz Mahmoudi Rad Ameneh Sh Zafarghandi Maryam Amel Zabihi Mahkam Tavallaee Yasaman Mirdamadi |
spellingShingle |
Mahnaz Mahmoudi Rad Ameneh Sh Zafarghandi Maryam Amel Zabihi Mahkam Tavallaee Yasaman Mirdamadi Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR Infectious Diseases in Obstetrics and Gynecology |
author_facet |
Mahnaz Mahmoudi Rad Ameneh Sh Zafarghandi Maryam Amel Zabihi Mahkam Tavallaee Yasaman Mirdamadi |
author_sort |
Mahnaz Mahmoudi Rad |
title |
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR |
title_short |
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR |
title_full |
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR |
title_fullStr |
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR |
title_full_unstemmed |
Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR |
title_sort |
identification of candida species associated with vulvovaginal candidiasis by multiplex pcr |
publisher |
Hindawi Limited |
series |
Infectious Diseases in Obstetrics and Gynecology |
issn |
1064-7449 1098-0997 |
publishDate |
2012-01-01 |
description |
Background. Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified. The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed. Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings. Results. C. albicans and C. glabrata were the most common species isolated from the specimens. A mix of C. glabrata and C. albicans was the most common mixed infection isolated from the samples. The analysis revealed a significant positive association between older age and infection with C. glabrata isolates (Spearman’s rho = 0.89, P=0.015). Conclusion. Multiplex PCR is a fast, yet reliable method to identify Candida species. C. albicans and then C. glabrata are the two most common causes of vulvovaginal candidiasis. The number of mixed fungal infections is higher among Iranian population compared to international reports. |
url |
http://dx.doi.org/10.1155/2012/872169 |
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