Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG
Background: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the vi...
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doaj-33ce20a0db464c0784e9bf46a30707b72020-11-24T21:59:11ZengElsevierInternational Journal of Infectious Diseases1201-97122019-04-01813137Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgGCarlos Palomares-Reyes0Wilmer Silva-Caso1Luis J. del Valle2Miguel Angel Aguilar-Luis3Claudia Weilg4Johanna Martins-Luna5Adriana Viñas-Ospino6Luciana Stimmler7Naysha Mallqui Espinoza8Ronald Aquino Ortega9Walter Espinoza Espíritu10Erika Misaico11Juana del Valle-Mendoza12School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Centro de Salud las Palmas, Red de Salud Leoncio Prado, Tingo María, PeruBarcelona Research Center for Multiscale Science and Engineering, Departament d’Enginyeria Quıímica EEBE, Universidad Politècnica de Catalunya (UPC), Barcelona, SpainSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru; Instituto de Investigación de Enfermedades Infecciosas, Lima, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, PeruLaboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, PeruUniversidad Nacional Agraria de la Selva Tingo María, Huánuco, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, PeruPuesto de Salud Tambillo Grande, Red de Salud Leoncio Prado, Huánuco, PeruHospital de Tingo María, Ministerio de Salud del Peru, Huánuco, PeruSchool of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru; Corresponding author at: Universidad Peruana de Ciencias Aplicadas, Av. San Marcos cuadra 2, Chorrillos, Lima, Peru.Background: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. Methods: A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. Results: DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods Conclusions: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru. Keywords: Dengue, DENV, Arbovirus, Diagnosis, Peruhttp://www.sciencedirect.com/science/article/pii/S1201971219300335 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlos Palomares-Reyes Wilmer Silva-Caso Luis J. del Valle Miguel Angel Aguilar-Luis Claudia Weilg Johanna Martins-Luna Adriana Viñas-Ospino Luciana Stimmler Naysha Mallqui Espinoza Ronald Aquino Ortega Walter Espinoza Espíritu Erika Misaico Juana del Valle-Mendoza |
spellingShingle |
Carlos Palomares-Reyes Wilmer Silva-Caso Luis J. del Valle Miguel Angel Aguilar-Luis Claudia Weilg Johanna Martins-Luna Adriana Viñas-Ospino Luciana Stimmler Naysha Mallqui Espinoza Ronald Aquino Ortega Walter Espinoza Espíritu Erika Misaico Juana del Valle-Mendoza Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG International Journal of Infectious Diseases |
author_facet |
Carlos Palomares-Reyes Wilmer Silva-Caso Luis J. del Valle Miguel Angel Aguilar-Luis Claudia Weilg Johanna Martins-Luna Adriana Viñas-Ospino Luciana Stimmler Naysha Mallqui Espinoza Ronald Aquino Ortega Walter Espinoza Espíritu Erika Misaico Juana del Valle-Mendoza |
author_sort |
Carlos Palomares-Reyes |
title |
Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
title_short |
Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
title_full |
Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
title_fullStr |
Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
title_full_unstemmed |
Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
title_sort |
dengue diagnosis in an endemic area of peru: clinical characteristics and positive frequencies by rt-pcr and serology for ns1, igm, and igg |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 |
publishDate |
2019-04-01 |
description |
Background: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. Methods: A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. Results: DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods Conclusions: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru. Keywords: Dengue, DENV, Arbovirus, Diagnosis, Peru |
url |
http://www.sciencedirect.com/science/article/pii/S1201971219300335 |
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