Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods
Abstract Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respirato...
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doaj-33da8b1c5ec943929ceabe2756ae9a372020-11-25T04:09:53ZengBMCVeterinary Research1297-97162020-11-0151111410.1186/s13567-020-00862-1Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methodsLukas Schuwerk0Doris Hoeltig1Karl-Heinz Waldmann2Katrin Strutzberg-Minder3Peter Valentin-Weigand4Judith Rohde5Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, FoundationClinic for Swine and Small Ruminants and Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, FoundationClinic for Swine and Small Ruminants and Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, FoundationInnovative Veterinary Diagnostics Laboratory (IVD GmbH)Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, FoundationInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, FoundationAbstract Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed. Isolates were characterized for serovar implementing two different PCR methods. Additionally, two different PCR methods for pathotyping isolates were applied to the collection and results compared. Serovar 6 (p < 0.0001) and 9 (p = 0.0007) were correlated with carrier isolates and serovar 4 was associated with isolates from animals with respiratory disease (p = 0.015). In systemic isolates, serovar 13 was most frequently detected (18.9%). Various other serovars were isolated from all sites and the ratio of serovar 5 to serovar 12 was approximately 1:2. These two serovars together represented 14.3% of the isolates; only serovar 4 was isolated more frequently (24.7%). The pathotyping method based on the leader sequence (LS = ESPR of vta) was easy to perform and corresponded well to the clinical background information. Of the carrier isolates 72% were identified as non-virulent while 91% of the systemic isolates were classified as virulent (p < 0.0001). Results of the pathotyping PCR based on 10 different marker genes overall were in good agreement with clinical metadata as well as with results of the LS-PCR. However, the pathotyping PCR was more complicated to perform and analyze. In conclusion, a combination of the serotyping multiplex-PCR and the LS-PCR could improve identification of clinically relevant G. parasuis isolates, especially from respiratory samples.http://link.springer.com/article/10.1186/s13567-020-00862-1Glaesserella parasuisserotypingpathotypingleader sequencevirulenceserovar |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lukas Schuwerk Doris Hoeltig Karl-Heinz Waldmann Katrin Strutzberg-Minder Peter Valentin-Weigand Judith Rohde |
spellingShingle |
Lukas Schuwerk Doris Hoeltig Karl-Heinz Waldmann Katrin Strutzberg-Minder Peter Valentin-Weigand Judith Rohde Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods Veterinary Research Glaesserella parasuis serotyping pathotyping leader sequence virulence serovar |
author_facet |
Lukas Schuwerk Doris Hoeltig Karl-Heinz Waldmann Katrin Strutzberg-Minder Peter Valentin-Weigand Judith Rohde |
author_sort |
Lukas Schuwerk |
title |
Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods |
title_short |
Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods |
title_full |
Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods |
title_fullStr |
Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods |
title_full_unstemmed |
Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods |
title_sort |
serotyping and pathotyping of glaesserella parasuis isolated 2012–2019 in germany comparing different pcr-based methods |
publisher |
BMC |
series |
Veterinary Research |
issn |
1297-9716 |
publishDate |
2020-11-01 |
description |
Abstract Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed. Isolates were characterized for serovar implementing two different PCR methods. Additionally, two different PCR methods for pathotyping isolates were applied to the collection and results compared. Serovar 6 (p < 0.0001) and 9 (p = 0.0007) were correlated with carrier isolates and serovar 4 was associated with isolates from animals with respiratory disease (p = 0.015). In systemic isolates, serovar 13 was most frequently detected (18.9%). Various other serovars were isolated from all sites and the ratio of serovar 5 to serovar 12 was approximately 1:2. These two serovars together represented 14.3% of the isolates; only serovar 4 was isolated more frequently (24.7%). The pathotyping method based on the leader sequence (LS = ESPR of vta) was easy to perform and corresponded well to the clinical background information. Of the carrier isolates 72% were identified as non-virulent while 91% of the systemic isolates were classified as virulent (p < 0.0001). Results of the pathotyping PCR based on 10 different marker genes overall were in good agreement with clinical metadata as well as with results of the LS-PCR. However, the pathotyping PCR was more complicated to perform and analyze. In conclusion, a combination of the serotyping multiplex-PCR and the LS-PCR could improve identification of clinically relevant G. parasuis isolates, especially from respiratory samples. |
topic |
Glaesserella parasuis serotyping pathotyping leader sequence virulence serovar |
url |
http://link.springer.com/article/10.1186/s13567-020-00862-1 |
work_keys_str_mv |
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