The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis
Serine protease inhibitors (SPI) are a superfamily of the proteins able to suppress serine protease activity, and may exert the major biological function in complement activation, inflammation, and fibrinolysis. A SPI was identified from Trichinella spiralis adult worms (AW) by immunoproteomics with...
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2018-07-01
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doaj-33e433d19bc141c6b93365aebe0222112020-11-24T22:59:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01544388303The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralisYan Y. SongYao ZhangDaqi YangHua N. RenGe G. SunPeng JiangRuo D. LiuXi ZhangJing CuiZhong Q. WangSerine protease inhibitors (SPI) are a superfamily of the proteins able to suppress serine protease activity, and may exert the major biological function in complement activation, inflammation, and fibrinolysis. A SPI was identified from Trichinella spiralis adult worms (AW) by immunoproteomics with early infection sera. The aim of this study was to investigate the protective immune elicited by TsSPI. The complete TsSPI cDNA sequence was cloned into pQE-80 L and then expressed in Escherichia coli BL21. The rTsSPI was purified and its antigenicity was determined by Western blotting analysis. By using anti-rTsSPI serum the native TsSPI was identified in somatic and ES proteins from muscle larvae (ML). The results of qPCR and immunofluorescence assay (IFA) revealed that the expression of the TsSPI gene was observed throughout all developmental stages of T. spiralis (ML, intestinal infective larvale, 3- and 6-days AW, and newborn larvae, NBL), located principally in cuticles, stichosome, and embryos of this parasitic nematode. Vaccination of mice with rTsSPI triggered high level of anti-TsSPI IgG response, and showed a 62.2 and 57.25% worm burden reduction in the recovery of intestinal AW at 6 days post-infection (dpi) and ML at 35 dpi, respectively. The TsSPI might be a novel potential target for anti-Trichinella vaccine.https://www.frontiersin.org/article/10.3389/fmicb.2018.01544/fullTrichinella spiralisserine protease inhibitorsidentificationtissue localizationimmune protection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Y. Song Yao Zhang Daqi Yang Hua N. Ren Ge G. Sun Peng Jiang Ruo D. Liu Xi Zhang Jing Cui Zhong Q. Wang |
spellingShingle |
Yan Y. Song Yao Zhang Daqi Yang Hua N. Ren Ge G. Sun Peng Jiang Ruo D. Liu Xi Zhang Jing Cui Zhong Q. Wang The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis Frontiers in Microbiology Trichinella spiralis serine protease inhibitors identification tissue localization immune protection |
author_facet |
Yan Y. Song Yao Zhang Daqi Yang Hua N. Ren Ge G. Sun Peng Jiang Ruo D. Liu Xi Zhang Jing Cui Zhong Q. Wang |
author_sort |
Yan Y. Song |
title |
The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis |
title_short |
The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis |
title_full |
The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis |
title_fullStr |
The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis |
title_full_unstemmed |
The Immune Protection Induced by a Serine Protease Inhibitor From the Foodborne Parasite Trichinella spiralis |
title_sort |
immune protection induced by a serine protease inhibitor from the foodborne parasite trichinella spiralis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-07-01 |
description |
Serine protease inhibitors (SPI) are a superfamily of the proteins able to suppress serine protease activity, and may exert the major biological function in complement activation, inflammation, and fibrinolysis. A SPI was identified from Trichinella spiralis adult worms (AW) by immunoproteomics with early infection sera. The aim of this study was to investigate the protective immune elicited by TsSPI. The complete TsSPI cDNA sequence was cloned into pQE-80 L and then expressed in Escherichia coli BL21. The rTsSPI was purified and its antigenicity was determined by Western blotting analysis. By using anti-rTsSPI serum the native TsSPI was identified in somatic and ES proteins from muscle larvae (ML). The results of qPCR and immunofluorescence assay (IFA) revealed that the expression of the TsSPI gene was observed throughout all developmental stages of T. spiralis (ML, intestinal infective larvale, 3- and 6-days AW, and newborn larvae, NBL), located principally in cuticles, stichosome, and embryos of this parasitic nematode. Vaccination of mice with rTsSPI triggered high level of anti-TsSPI IgG response, and showed a 62.2 and 57.25% worm burden reduction in the recovery of intestinal AW at 6 days post-infection (dpi) and ML at 35 dpi, respectively. The TsSPI might be a novel potential target for anti-Trichinella vaccine. |
topic |
Trichinella spiralis serine protease inhibitors identification tissue localization immune protection |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01544/full |
work_keys_str_mv |
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