Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia

Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia

An induced pluripotent stem cell (iPSC) line, MUSIi010-A, was established by Sendai virus (SeV) transduction of scalp fibroblasts from a 59-year-old male with androgenetic alopecia (AGA). Pluripotency of the iPSC line was verified by immunofluorescence staining of pluripotent markers and by in vitro...

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Main Authors: Nutnicha Tantarungsee, Zaukir Bostan Ali, Rungtip Soi-ampornkul, Chuda Rujitharanawong, Chanika Subchookul, Alisa Ritthaphai, Bootsakorn Boonkaew, Methichit Wattanapanitch, Kanchalit Thanomkitti, Daranporn Triwongwaranat, Chinnavuth Vatanashevanopakorn, Rattapon Thuangtong
Format: Article
Language:English
Published: Elsevier 2019-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119301163
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spelling doaj-33e5d7df0a4246d08700033bebf8f3b72020-11-25T00:47:47ZengElsevierStem Cell Research1873-50612019-07-0138Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopeciaNutnicha Tantarungsee0Zaukir Bostan Ali1Rungtip Soi-ampornkul2Chuda Rujitharanawong3Chanika Subchookul4Alisa Ritthaphai5Bootsakorn Boonkaew6Methichit Wattanapanitch7Kanchalit Thanomkitti8Daranporn Triwongwaranat9Chinnavuth Vatanashevanopakorn10Rattapon Thuangtong11Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding authors.Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding authors.An induced pluripotent stem cell (iPSC) line, MUSIi010-A, was established by Sendai virus (SeV) transduction of scalp fibroblasts from a 59-year-old male with androgenetic alopecia (AGA). Pluripotency of the iPSC line was verified by immunofluorescence staining of pluripotent markers and by in vitro trilineage differentiation. The MUSIi010-A line was shown to retain normal karyotype and free of SeV vectors at passage 17. This iPSC line can be used for studying pathological mechanisms of AGA.http://www.sciencedirect.com/science/article/pii/S1873506119301163
collection DOAJ
language English
format Article
sources DOAJ
author Nutnicha Tantarungsee
Zaukir Bostan Ali
Rungtip Soi-ampornkul
Chuda Rujitharanawong
Chanika Subchookul
Alisa Ritthaphai
Bootsakorn Boonkaew
Methichit Wattanapanitch
Kanchalit Thanomkitti
Daranporn Triwongwaranat
Chinnavuth Vatanashevanopakorn
Rattapon Thuangtong
spellingShingle Nutnicha Tantarungsee
Zaukir Bostan Ali
Rungtip Soi-ampornkul
Chuda Rujitharanawong
Chanika Subchookul
Alisa Ritthaphai
Bootsakorn Boonkaew
Methichit Wattanapanitch
Kanchalit Thanomkitti
Daranporn Triwongwaranat
Chinnavuth Vatanashevanopakorn
Rattapon Thuangtong
Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
Stem Cell Research
author_facet Nutnicha Tantarungsee
Zaukir Bostan Ali
Rungtip Soi-ampornkul
Chuda Rujitharanawong
Chanika Subchookul
Alisa Ritthaphai
Bootsakorn Boonkaew
Methichit Wattanapanitch
Kanchalit Thanomkitti
Daranporn Triwongwaranat
Chinnavuth Vatanashevanopakorn
Rattapon Thuangtong
author_sort Nutnicha Tantarungsee
title Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
title_short Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
title_full Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
title_fullStr Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
title_full_unstemmed Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia
title_sort generation of an integration-free human induced pluripotent stem cell line musii010-a from occipital scalp fibroblasts of a male patient with androgenetic alopecia
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2019-07-01
description An induced pluripotent stem cell (iPSC) line, MUSIi010-A, was established by Sendai virus (SeV) transduction of scalp fibroblasts from a 59-year-old male with androgenetic alopecia (AGA). Pluripotency of the iPSC line was verified by immunofluorescence staining of pluripotent markers and by in vitro trilineage differentiation. The MUSIi010-A line was shown to retain normal karyotype and free of SeV vectors at passage 17. This iPSC line can be used for studying pathological mechanisms of AGA.
url http://www.sciencedirect.com/science/article/pii/S1873506119301163
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