Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid
The resistin gene is expressed in adipocytes and encodes a protein proposed to link obesity and type 2 diabetes. Increased plasma FFA is associated with insulin resistance. We examined the effect of separate FFAs on the expression of resistin mRNA in cultured murine 3T3-L1 adipocytes. The FFAs teste...
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doaj-33ff1970d94e4b91adcfd4e8363946752021-04-27T04:46:41ZengElsevierJournal of Lipid Research0022-22752005-01-01461143153Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acidFred Haugen0Naeem Zahid1Knut T. Dalen2Kristin Hollung3Hilde I. Nebb4Christian A. Drevon5Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayThe resistin gene is expressed in adipocytes and encodes a protein proposed to link obesity and type 2 diabetes. Increased plasma FFA is associated with insulin resistance. We examined the effect of separate FFAs on the expression of resistin mRNA in cultured murine 3T3-L1 adipocytes. The FFAs tested did not increase resistin expression, whereas both arachidonic acid (AA) and eicosapentaenoic acid (EPA) reduced resistin mRNA levels. AA was by far the most potent FFA, reducing resistin mRNA levels to ∼20% of control at 60–250 μM concentration. Selective inhibitors of cyclooxygenase-1 and of mitogen-activated protein kinase kinase counteracted AA-induced reduction in resistin mRNA levels. Transient overexpression of sterol-regulatory element binding protein-1a (SREBP-1a) activated the resistin promoter, but there was no reduction in the abundance of ∼65 kDa mature SREBP-1 after AA exposure. Actinomycin D as well as cycloheximide abolished the AA-induced reduction of resistin mRNA levels, indicating dependence on de novo transcription and translation.Our data suggest that reductions in resistin mRNA levels involve a destabilization of the resistin mRNA molecule. An inhibitory effect of AA and EPA on resistin expression may explain the beneficial effect of ingesting PUFAs on insulin sensitivity.http://www.sciencedirect.com/science/article/pii/S0022227520340906fatty acidinsulin resistanceperoxisome proliferator-activated receptor γliver X receptor αsterol-regulatory element binding protein-1polyunsaturated fatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fred Haugen Naeem Zahid Knut T. Dalen Kristin Hollung Hilde I. Nebb Christian A. Drevon |
spellingShingle |
Fred Haugen Naeem Zahid Knut T. Dalen Kristin Hollung Hilde I. Nebb Christian A. Drevon Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid Journal of Lipid Research fatty acid insulin resistance peroxisome proliferator-activated receptor γ liver X receptor α sterol-regulatory element binding protein-1 polyunsaturated fatty acids |
author_facet |
Fred Haugen Naeem Zahid Knut T. Dalen Kristin Hollung Hilde I. Nebb Christian A. Drevon |
author_sort |
Fred Haugen |
title |
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid |
title_short |
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid |
title_full |
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid |
title_fullStr |
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid |
title_full_unstemmed |
Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid |
title_sort |
resistin expression in 3t3-l1 adipocytes is reduced by arachidonic acid |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2005-01-01 |
description |
The resistin gene is expressed in adipocytes and encodes a protein proposed to link obesity and type 2 diabetes. Increased plasma FFA is associated with insulin resistance. We examined the effect of separate FFAs on the expression of resistin mRNA in cultured murine 3T3-L1 adipocytes. The FFAs tested did not increase resistin expression, whereas both arachidonic acid (AA) and eicosapentaenoic acid (EPA) reduced resistin mRNA levels. AA was by far the most potent FFA, reducing resistin mRNA levels to ∼20% of control at 60–250 μM concentration. Selective inhibitors of cyclooxygenase-1 and of mitogen-activated protein kinase kinase counteracted AA-induced reduction in resistin mRNA levels. Transient overexpression of sterol-regulatory element binding protein-1a (SREBP-1a) activated the resistin promoter, but there was no reduction in the abundance of ∼65 kDa mature SREBP-1 after AA exposure. Actinomycin D as well as cycloheximide abolished the AA-induced reduction of resistin mRNA levels, indicating dependence on de novo transcription and translation.Our data suggest that reductions in resistin mRNA levels involve a destabilization of the resistin mRNA molecule. An inhibitory effect of AA and EPA on resistin expression may explain the beneficial effect of ingesting PUFAs on insulin sensitivity. |
topic |
fatty acid insulin resistance peroxisome proliferator-activated receptor γ liver X receptor α sterol-regulatory element binding protein-1 polyunsaturated fatty acids |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520340906 |
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