Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV
Background: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variation...
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doaj-340cea557403404a952c86ce9f817fe12021-04-24T23:02:35ZengMDPI AGPathogens2076-08172021-04-011051851810.3390/pathogens10050518Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIVWilfried Wenceslas Bazié0Benjamin Goyer1Julien Boucher2Yuwei Zhang3Delphine Planas4Debashree Chatterjee5Jean-Pierre Routy6Michel Alary7Petronela Ancuta8Caroline Gilbert9Axe de Recherche Maladies Infectieuses et Immunitaires, CHUL, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1V 4G2, CanadaAxe de Recherche Maladies Infectieuses et Immunitaires, CHUL, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1V 4G2, CanadaAxe de Recherche Maladies Infectieuses et Immunitaires, CHUL, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1V 4G2, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaChronic Viral Illness Service and Division of Hematology, McGill University Health Centre, Montréal, QC H4A 3J1, CanadaAxe de Recherche Santé des Populations et Pratiques Optimales en Santé, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1S 4L8, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaAxe de Recherche Maladies Infectieuses et Immunitaires, CHUL, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1V 4G2, CanadaBackground: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV−). Methods: Venous blood samples from n = 10 HIV+ART and n = 10 HIV− participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Results: Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day–night increase in the quantity of 5 miRNAs in HIV− large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV− to HIV+ART in multivariate analysis. Conclusion: These results point that plasma EV amount and microRNA contents are under daily variation in HIV− people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers.https://www.mdpi.com/2076-0817/10/5/518extracellular vesiclesHIV−1microRNAmiR-29amiR-29bmiR-92 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wilfried Wenceslas Bazié Benjamin Goyer Julien Boucher Yuwei Zhang Delphine Planas Debashree Chatterjee Jean-Pierre Routy Michel Alary Petronela Ancuta Caroline Gilbert |
spellingShingle |
Wilfried Wenceslas Bazié Benjamin Goyer Julien Boucher Yuwei Zhang Delphine Planas Debashree Chatterjee Jean-Pierre Routy Michel Alary Petronela Ancuta Caroline Gilbert Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV Pathogens extracellular vesicles HIV−1 microRNA miR-29a miR-29b miR-92 |
author_facet |
Wilfried Wenceslas Bazié Benjamin Goyer Julien Boucher Yuwei Zhang Delphine Planas Debashree Chatterjee Jean-Pierre Routy Michel Alary Petronela Ancuta Caroline Gilbert |
author_sort |
Wilfried Wenceslas Bazié |
title |
Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV |
title_short |
Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV |
title_full |
Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV |
title_fullStr |
Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV |
title_full_unstemmed |
Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV |
title_sort |
diurnal variation of plasma extracellular vesicle is disrupted in people living with hiv |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2021-04-01 |
description |
Background: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV−). Methods: Venous blood samples from n = 10 HIV+ART and n = 10 HIV− participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Results: Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day–night increase in the quantity of 5 miRNAs in HIV− large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV− to HIV+ART in multivariate analysis. Conclusion: These results point that plasma EV amount and microRNA contents are under daily variation in HIV− people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers. |
topic |
extracellular vesicles HIV−1 microRNA miR-29a miR-29b miR-92 |
url |
https://www.mdpi.com/2076-0817/10/5/518 |
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