Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips?
Shoot tips of Potato (Solanum phureja) were cryopreserved with the encapsulation-dehydration method, including: coating of shoot tips in calcium alginate beads, pre-culture in a solution containing 0.75 M of sugar (0.1 M of sucrose +0.65 M of another cryoprotectant sugar), and dehydration (for 4.5 h...
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doaj-3428bf6aaabd4f31a342f1586ae883b02020-11-25T02:22:55ZengElsevierJournal of King Saud University: Science1018-36472020-04-0132319171924Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips?Samia Bissati0Saliha Boudjenah1Christiane Morisset2Haroun Chenchouni3Laboratoire des Bioressources sahariennes, Université Kasdi Merbah Ouargla, 30000 Ouargla, AlgeriaLaboratoire de Recherche sur la Phoeniciculture, Faculté des Sciences de la Nature et de la Vie, Université Kasdi Merbah Ouargla, 30000 Ouargla, AlgeriaLaboratoire de Cryobiologie Végétale, Université Pierre et Marie Curie, Paris 6, FranceDepartment of Natural and Life Sciences, Faculty of Exact Sciences and Natural and Life Sciences, University of Tebessa, 12000 Tebessa, Algeria; Laboratory of Natural Resources and Management of Sensitive Environments ‘RNAMS’, University of Oum-El-Bouaghi, 04000 Oum-El-Bouaghi, Algeria; Corresponding author at: Department of Natural and Life Sciences, Faculty of Exact Sciences and Natural and Life Sciences, University of Tebessa, 12000 Tebessa, Algeria.Shoot tips of Potato (Solanum phureja) were cryopreserved with the encapsulation-dehydration method, including: coating of shoot tips in calcium alginate beads, pre-culture in a solution containing 0.75 M of sugar (0.1 M of sucrose +0.65 M of another cryoprotectant sugar), and dehydration (for 4.5 h) on silica gel to reduce water content to 21–27%. Regrowth of shoot tips was assessed after freezing in liquid nitrogen. After cryopreservation, coated shoot tips were cultivated on a medium enriched with growth substances to measure concentrations of soluble carbohydrates. Maltose and trehalose ensured a better cryopreservation (88% and 91% of regrowth, respectively). Regrowth rates of potato shoot tips in the presence of polyols were very low and no recovery was obtained after freezing of shoot tips that were pre-cultivated in monosaccharides. Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose, especially when sucrose was applied as the cryoprotectant. The application of sucrose helped potato shoot-tips to acquire a certain tolerance to dehydration and cryofreezing. The analysis of soluble carbohydrates in potato shoot tips confirmed the accumulation of sugars during the pre-culture process, principally in the form of sucrose. The statistical analysis showed that soluble carbohydrate contents in shoot tips increased when total carbohydrates increased in the cultivation medium. The modelling approach indicated that the contents of soluble carbohydrates increased when pre-cultivation of potato shoot-tips was conducted in sucrose media compared to the control and other sugars. Keywords: Cryopreservation, Pre-culture, Soluble carbohydrates, Liquid nitrogen, Coated shoot tips, Encapsulation-dehydration, Potatohttp://www.sciencedirect.com/science/article/pii/S1018364720300471 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Samia Bissati Saliha Boudjenah Christiane Morisset Haroun Chenchouni |
spellingShingle |
Samia Bissati Saliha Boudjenah Christiane Morisset Haroun Chenchouni Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? Journal of King Saud University: Science |
author_facet |
Samia Bissati Saliha Boudjenah Christiane Morisset Haroun Chenchouni |
author_sort |
Samia Bissati |
title |
Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? |
title_short |
Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? |
title_full |
Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? |
title_fullStr |
Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? |
title_full_unstemmed |
Does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (Solanum phureja) shoot tips? |
title_sort |
does pre-culture in sugar-rich media affect carbohydrate content and post-thawing recovery rate of cryopreserved potato (solanum phureja) shoot tips? |
publisher |
Elsevier |
series |
Journal of King Saud University: Science |
issn |
1018-3647 |
publishDate |
2020-04-01 |
description |
Shoot tips of Potato (Solanum phureja) were cryopreserved with the encapsulation-dehydration method, including: coating of shoot tips in calcium alginate beads, pre-culture in a solution containing 0.75 M of sugar (0.1 M of sucrose +0.65 M of another cryoprotectant sugar), and dehydration (for 4.5 h) on silica gel to reduce water content to 21–27%. Regrowth of shoot tips was assessed after freezing in liquid nitrogen. After cryopreservation, coated shoot tips were cultivated on a medium enriched with growth substances to measure concentrations of soluble carbohydrates. Maltose and trehalose ensured a better cryopreservation (88% and 91% of regrowth, respectively). Regrowth rates of potato shoot tips in the presence of polyols were very low and no recovery was obtained after freezing of shoot tips that were pre-cultivated in monosaccharides. Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose, especially when sucrose was applied as the cryoprotectant. The application of sucrose helped potato shoot-tips to acquire a certain tolerance to dehydration and cryofreezing. The analysis of soluble carbohydrates in potato shoot tips confirmed the accumulation of sugars during the pre-culture process, principally in the form of sucrose. The statistical analysis showed that soluble carbohydrate contents in shoot tips increased when total carbohydrates increased in the cultivation medium. The modelling approach indicated that the contents of soluble carbohydrates increased when pre-cultivation of potato shoot-tips was conducted in sucrose media compared to the control and other sugars. Keywords: Cryopreservation, Pre-culture, Soluble carbohydrates, Liquid nitrogen, Coated shoot tips, Encapsulation-dehydration, Potato |
url |
http://www.sciencedirect.com/science/article/pii/S1018364720300471 |
work_keys_str_mv |
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