Derivation of FSHD1 affected human embryonic stem cell line Genea050

Derivation of FSHD1 affected human embryonic stem cell line Genea050

The Genea050 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying a deletion in 4q35 with only 5 D4Z4 repeats by PGD linkage analysis, indicative of FSHD1. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46,...

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Main Authors: Biljana Dumevska, Omar Chami, Heather Main, Robert McKernan, Divya Goel, Uli Schmidt
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000659
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spelling doaj-342fc31674dd40408befaa2514e1d3712020-11-24T21:10:43ZengElsevierStem Cell Research1873-50611876-77532016-03-0116250350610.1016/j.scr.2016.02.023Derivation of FSHD1 affected human embryonic stem cell line Genea050Biljana DumevskaOmar ChamiHeather MainRobert McKernanDivya GoelUli SchmidtThe Genea050 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying a deletion in 4q35 with only 5 D4Z4 repeats by PGD linkage analysis, indicative of FSHD1. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 92% of cells expressed Nanog, 97% Oct4, 79% Tra1-60 and 99% SSEA4, gave a Pluritest Pluripotency score of 25.45, Novelty of 1.45 demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.http://www.sciencedirect.com/science/article/pii/S1873506116000659
collection DOAJ
language English
format Article
sources DOAJ
author Biljana Dumevska
Omar Chami
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
spellingShingle Biljana Dumevska
Omar Chami
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
Derivation of FSHD1 affected human embryonic stem cell line Genea050
Stem Cell Research
author_facet Biljana Dumevska
Omar Chami
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
author_sort Biljana Dumevska
title Derivation of FSHD1 affected human embryonic stem cell line Genea050
title_short Derivation of FSHD1 affected human embryonic stem cell line Genea050
title_full Derivation of FSHD1 affected human embryonic stem cell line Genea050
title_fullStr Derivation of FSHD1 affected human embryonic stem cell line Genea050
title_full_unstemmed Derivation of FSHD1 affected human embryonic stem cell line Genea050
title_sort derivation of fshd1 affected human embryonic stem cell line genea050
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The Genea050 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying a deletion in 4q35 with only 5 D4Z4 repeats by PGD linkage analysis, indicative of FSHD1. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 92% of cells expressed Nanog, 97% Oct4, 79% Tra1-60 and 99% SSEA4, gave a Pluritest Pluripotency score of 25.45, Novelty of 1.45 demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
url http://www.sciencedirect.com/science/article/pii/S1873506116000659
work_keys_str_mv AT biljanadumevska derivationoffshd1affectedhumanembryonicstemcelllinegenea050
AT omarchami derivationoffshd1affectedhumanembryonicstemcelllinegenea050
AT heathermain derivationoffshd1affectedhumanembryonicstemcelllinegenea050
AT robertmckernan derivationoffshd1affectedhumanembryonicstemcelllinegenea050
AT divyagoel derivationoffshd1affectedhumanembryonicstemcelllinegenea050
AT ulischmidt derivationoffshd1affectedhumanembryonicstemcelllinegenea050
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