Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein
A B S T R A C T Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by s...
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Universitas Sriwijaya
2021-01-01
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| Series: | Bioscientia Medicina: Journal of Biomedicine and Translational Research |
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| Online Access: | https://bioscmed.com/index.php/bsm/article/view/229 |
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doaj-346371d7d9a1431e832c24261814158a2021-02-11T14:21:42ZengUniversitas SriwijayaBioscientia Medicina: Journal of Biomedicine and Translational Research2598-05802021-01-015235937210.32539/bsm.v5i2.229229Western Blotting (WB) Technique Guideline for Separation and Isolation of ProteinRachmat Hidayat0Patricia Wulandari1Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, IndonesiaCattleya Mental Health Center, Palembang, IndonesiaA B S T R A C T Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problemhttps://bioscmed.com/index.php/bsm/article/view/229western blottingproteinantibody |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Rachmat Hidayat Patricia Wulandari |
| spellingShingle |
Rachmat Hidayat Patricia Wulandari Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein Bioscientia Medicina: Journal of Biomedicine and Translational Research western blotting protein antibody |
| author_facet |
Rachmat Hidayat Patricia Wulandari |
| author_sort |
Rachmat Hidayat |
| title |
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein |
| title_short |
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein |
| title_full |
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein |
| title_fullStr |
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein |
| title_full_unstemmed |
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein |
| title_sort |
western blotting (wb) technique guideline for separation and isolation of protein |
| publisher |
Universitas Sriwijaya |
| series |
Bioscientia Medicina: Journal of Biomedicine and Translational Research |
| issn |
2598-0580 |
| publishDate |
2021-01-01 |
| description |
A B S T R A C T
Western blotting is an important technique used in cell and molecular
biology. Using the western blot, researchers can identify specific proteins
from the complex mixture of proteins extracted from cells. This technique
uses three elements to accomplish this task: (1) separation by size, (2)
transfer to a solid support, and (3) marking target proteins using appropriate
primary and secondary antibodies to visualize. This paper will attempt to
explain the techniques and theory behind western blot, and offer several
ways to solve the problem |
| topic |
western blotting protein antibody |
| url |
https://bioscmed.com/index.php/bsm/article/view/229 |
| work_keys_str_mv |
AT rachmathidayat westernblottingwbtechniqueguidelineforseparationandisolationofprotein AT patriciawulandari westernblottingwbtechniqueguidelineforseparationandisolationofprotein |
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