Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma
The epithelial mesenchymal transition (EMT) is a physiological multistep process involving epithelial cells acquiring a mesenchymal-like phenotype. It is widely demonstrated that EMT is linked to tumor progression and metastasis. The transforming growth factor (TGF)-β pathways have been widely inves...
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doaj-34667b5d50ff44058a3b3727303517e02020-11-24T23:03:48ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-12-0120110810.3390/ijms20010108ijms20010108Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular CarcinomaMariafrancesca Cascione0Stefano Leporatti1Francesco Dituri2Gianluigi Giannelli3Department of the Medical Sciences and Human Oncology, University Aldo Moro, 70124 Bari, ItalyCNR Nanotec—Institute of Nanotechnology, 73100 Lecce, ItalyNational Institute of Gastroenterology “S. de Bellis” Research Hospital, 70013 Castellana Grotte (Ba), ItalyNational Institute of Gastroenterology “S. de Bellis” Research Hospital, 70013 Castellana Grotte (Ba), ItalyThe epithelial mesenchymal transition (EMT) is a physiological multistep process involving epithelial cells acquiring a mesenchymal-like phenotype. It is widely demonstrated that EMT is linked to tumor progression and metastasis. The transforming growth factor (TGF)-β pathways have been widely investigated, but its role in the hepatocarcinoma EMT is still unclear. While the biochemical pathways have been extensively studied, the alteration of biomechanical behavior correlated to cellular phenotype and motility is not yet fully understood. To better define the involvement of TGF-β1 in the metastatic progression process in different hepatocarcinoma cell lines (HepG2, PLC/PRF/5, HLE), we applied a systematic morphomechanical approach in order to investigate the physical and the structural characteristics. In addition, we evaluated the antitumor effect of LY2157299, a TGF-βR1 kinase inhibitor, from a biomechanical point of view, using Atomic Force and Confocal Microscopy. Our approach allows for validation of biological data, therefore it may be used in the future as a diagnostic tool to be combined with conventional biomolecular techniques.http://www.mdpi.com/1422-0067/20/1/108TGF-β1epithelial to mesenchymal transition (EMT)atomic force microscopy (AFM) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariafrancesca Cascione Stefano Leporatti Francesco Dituri Gianluigi Giannelli |
spellingShingle |
Mariafrancesca Cascione Stefano Leporatti Francesco Dituri Gianluigi Giannelli Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma International Journal of Molecular Sciences TGF-β1 epithelial to mesenchymal transition (EMT) atomic force microscopy (AFM) |
author_facet |
Mariafrancesca Cascione Stefano Leporatti Francesco Dituri Gianluigi Giannelli |
author_sort |
Mariafrancesca Cascione |
title |
Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma |
title_short |
Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma |
title_full |
Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma |
title_fullStr |
Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma |
title_full_unstemmed |
Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma |
title_sort |
transforming growth factor-β promotes morphomechanical effects involved in epithelial to mesenchymal transition in living hepatocellular carcinoma |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-12-01 |
description |
The epithelial mesenchymal transition (EMT) is a physiological multistep process involving epithelial cells acquiring a mesenchymal-like phenotype. It is widely demonstrated that EMT is linked to tumor progression and metastasis. The transforming growth factor (TGF)-β pathways have been widely investigated, but its role in the hepatocarcinoma EMT is still unclear. While the biochemical pathways have been extensively studied, the alteration of biomechanical behavior correlated to cellular phenotype and motility is not yet fully understood. To better define the involvement of TGF-β1 in the metastatic progression process in different hepatocarcinoma cell lines (HepG2, PLC/PRF/5, HLE), we applied a systematic morphomechanical approach in order to investigate the physical and the structural characteristics. In addition, we evaluated the antitumor effect of LY2157299, a TGF-βR1 kinase inhibitor, from a biomechanical point of view, using Atomic Force and Confocal Microscopy. Our approach allows for validation of biological data, therefore it may be used in the future as a diagnostic tool to be combined with conventional biomolecular techniques. |
topic |
TGF-β1 epithelial to mesenchymal transition (EMT) atomic force microscopy (AFM) |
url |
http://www.mdpi.com/1422-0067/20/1/108 |
work_keys_str_mv |
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