Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila

Abstract The effect of chrysophanic acid (CA) (2, 4, and 8 mg kg−1) on the immunity and immune-related gene profile of Catla catla against Aeromonas hydrophila is reported. In both control and treated groups fed with 2 mg kg−1 (2 CA), the phagocytosis, hemolytic, myeloperoxidase content, and superox...

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Main Authors: Ramasamy Harikrishnan, Gunapathy Devi, Chellam Balasundaram, Hien Van Doan, Sanchai Jaturasitha, Einar Ringø, Caterina Faggio
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-79629-9
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spelling doaj-346b7f80eeb248adb13d6272e25dcd682021-01-17T12:41:55ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111510.1038/s41598-020-79629-9Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophilaRamasamy Harikrishnan0Gunapathy Devi1Chellam Balasundaram2Hien Van Doan3Sanchai Jaturasitha4Einar Ringø5Caterina Faggio6Department of Zoology, Pachaiyappa’s College for MenDepartment of Zoology, Nehru Memorial CollegeDepartment of Herbal and Environmental Science, Tamil UniversityDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai UniversityDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai UniversityNorwegian College of Fishery Science, Faculty of Bioscience, Fisheries and Economics, UiT The Arctic University of NorwayDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of MessinaAbstract The effect of chrysophanic acid (CA) (2, 4, and 8 mg kg−1) on the immunity and immune-related gene profile of Catla catla against Aeromonas hydrophila is reported. In both control and treated groups fed with 2 mg kg−1 (2 CA), the phagocytosis, hemolytic, myeloperoxidase content, and superoxide anion production decreased significantly between 6th and 8th weeks, whereas when fed with 4 mg kg−1 CA (4 CA) the H2O2 production and nitric oxide synthase increased significantly between 4th and 8th week. When fed with 2 CA and 4 CA diets, the total protein, bactericidal, and antibody titer increased significantly from the 4th week onwards. When fed with 2 CA, the IL-1β and IL-10 mRNA expression of head kidney leucocytes were significant between weeks 6 and 8. The expressions of toll-like receptors significantly increased when fed with a 4 CA diet from 4th week onwards. The 4 CA group significantly increased in TNF-α, TNF receptor-associated factor 6 (NOD), which influences protein expression, after the 4th week. The mRNA transcription of MHCI, lysozyme-chicken and goose type expressions significantly increased in 4 CA group within the 4th week. In summary, the dietary administration of 4 mg kg−1 of CA (4 CA) provides better immunity and enhances the up-regulation of immune-related genes in Catla against A. hydrophila.https://doi.org/10.1038/s41598-020-79629-9
collection DOAJ
language English
format Article
sources DOAJ
author Ramasamy Harikrishnan
Gunapathy Devi
Chellam Balasundaram
Hien Van Doan
Sanchai Jaturasitha
Einar Ringø
Caterina Faggio
spellingShingle Ramasamy Harikrishnan
Gunapathy Devi
Chellam Balasundaram
Hien Van Doan
Sanchai Jaturasitha
Einar Ringø
Caterina Faggio
Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
Scientific Reports
author_facet Ramasamy Harikrishnan
Gunapathy Devi
Chellam Balasundaram
Hien Van Doan
Sanchai Jaturasitha
Einar Ringø
Caterina Faggio
author_sort Ramasamy Harikrishnan
title Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
title_short Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
title_full Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
title_fullStr Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
title_full_unstemmed Effect of chrysophanic acid on immune response and immune genes transcriptomic profile in Catla catla against Aeromonas hydrophila
title_sort effect of chrysophanic acid on immune response and immune genes transcriptomic profile in catla catla against aeromonas hydrophila
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-01-01
description Abstract The effect of chrysophanic acid (CA) (2, 4, and 8 mg kg−1) on the immunity and immune-related gene profile of Catla catla against Aeromonas hydrophila is reported. In both control and treated groups fed with 2 mg kg−1 (2 CA), the phagocytosis, hemolytic, myeloperoxidase content, and superoxide anion production decreased significantly between 6th and 8th weeks, whereas when fed with 4 mg kg−1 CA (4 CA) the H2O2 production and nitric oxide synthase increased significantly between 4th and 8th week. When fed with 2 CA and 4 CA diets, the total protein, bactericidal, and antibody titer increased significantly from the 4th week onwards. When fed with 2 CA, the IL-1β and IL-10 mRNA expression of head kidney leucocytes were significant between weeks 6 and 8. The expressions of toll-like receptors significantly increased when fed with a 4 CA diet from 4th week onwards. The 4 CA group significantly increased in TNF-α, TNF receptor-associated factor 6 (NOD), which influences protein expression, after the 4th week. The mRNA transcription of MHCI, lysozyme-chicken and goose type expressions significantly increased in 4 CA group within the 4th week. In summary, the dietary administration of 4 mg kg−1 of CA (4 CA) provides better immunity and enhances the up-regulation of immune-related genes in Catla against A. hydrophila.
url https://doi.org/10.1038/s41598-020-79629-9
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