Nephron progenitor commitment is a stochastic process influenced by cell migration

Progenitor self-renewal and differentiation is often regulated by spatially restricted cues within a tissue microenvironment. Here, we examine how progenitor cell migration impacts regionally induced commitment within the nephrogenic niche in mice. We identify a subset of cells that express Wnt4, an...

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Main Authors:	Kynan T Lawlor, Luke Zappia, James Lefevre, Joo-Seop Park, Nicholas A Hamilton, Alicia Oshlack, Melissa H Little, Alexander N Combes
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2019-01-01
Series:	eLife
Subjects:	kidney development nephron progenitor cell migration single cell transcriptional profiling Wnt4 stochastic induction
Online Access:	https://elifesciences.org/articles/41156

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spelling	doaj-346dcaa82adf4a93883b05f34c9ad7032021-05-05T17:20:42ZengeLife Sciences Publications LtdeLife2050-084X2019-01-01810.7554/eLife.41156Nephron progenitor commitment is a stochastic process influenced by cell migrationKynan T Lawlor0https://orcid.org/0000-0003-4080-5439Luke Zappia1https://orcid.org/0000-0001-7744-8565James Lefevre2Joo-Seop Park3Nicholas A Hamilton4https://orcid.org/0000-0003-0331-3427Alicia Oshlack5https://orcid.org/0000-0001-9788-5690Melissa H Little6Alexander N Combes7https://orcid.org/0000-0001-6008-8786Murdoch Children's Research Institute, Parkville, AustraliaMurdoch Children's Research Institute, Parkville, Australia; School of Biosciences, University of Melbourne, Melbourne, AustraliaDivision of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, AustraliaDivision of Pediatric Urology and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, United StatesDivision of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, AustraliaMurdoch Children's Research Institute, Parkville, Australia; School of Biosciences, University of Melbourne, Melbourne, AustraliaMurdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, AustraliaMurdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, AustraliaProgenitor self-renewal and differentiation is often regulated by spatially restricted cues within a tissue microenvironment. Here, we examine how progenitor cell migration impacts regionally induced commitment within the nephrogenic niche in mice. We identify a subset of cells that express Wnt4, an early marker of nephron commitment, but migrate back into the progenitor population where they accumulate over time. Single cell RNA-seq and computational modelling of returning cells reveals that nephron progenitors can traverse the transcriptional hierarchy between self-renewal and commitment in either direction. This plasticity may enable robust regulation of nephrogenesis as niches remodel and grow during organogenesis.https://elifesciences.org/articles/41156kidney developmentnephron progenitorcell migrationsingle cell transcriptional profilingWnt4stochastic induction
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kynan T Lawlor Luke Zappia James Lefevre Joo-Seop Park Nicholas A Hamilton Alicia Oshlack Melissa H Little Alexander N Combes
spellingShingle	Kynan T Lawlor Luke Zappia James Lefevre Joo-Seop Park Nicholas A Hamilton Alicia Oshlack Melissa H Little Alexander N Combes Nephron progenitor commitment is a stochastic process influenced by cell migration eLife kidney development nephron progenitor cell migration single cell transcriptional profiling Wnt4 stochastic induction
author_facet	Kynan T Lawlor Luke Zappia James Lefevre Joo-Seop Park Nicholas A Hamilton Alicia Oshlack Melissa H Little Alexander N Combes
author_sort	Kynan T Lawlor
title	Nephron progenitor commitment is a stochastic process influenced by cell migration
title_short	Nephron progenitor commitment is a stochastic process influenced by cell migration
title_full	Nephron progenitor commitment is a stochastic process influenced by cell migration
title_fullStr	Nephron progenitor commitment is a stochastic process influenced by cell migration
title_full_unstemmed	Nephron progenitor commitment is a stochastic process influenced by cell migration
title_sort	nephron progenitor commitment is a stochastic process influenced by cell migration
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2019-01-01
description	Progenitor self-renewal and differentiation is often regulated by spatially restricted cues within a tissue microenvironment. Here, we examine how progenitor cell migration impacts regionally induced commitment within the nephrogenic niche in mice. We identify a subset of cells that express Wnt4, an early marker of nephron commitment, but migrate back into the progenitor population where they accumulate over time. Single cell RNA-seq and computational modelling of returning cells reveals that nephron progenitors can traverse the transcriptional hierarchy between self-renewal and commitment in either direction. This plasticity may enable robust regulation of nephrogenesis as niches remodel and grow during organogenesis.
topic	kidney development nephron progenitor cell migration single cell transcriptional profiling Wnt4 stochastic induction
url	https://elifesciences.org/articles/41156
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Nephron progenitor commitment is a stochastic process influenced by cell migration

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