Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination
Eight-carbon (C8) volatiles, such as 1-octen-3-ol, are ubiquitous among fungi. They are the volatiles critical for aroma and flavor of fungi, and assumed to be signals controlling germination of several fungi. In this study, we found that intact Aspergillus flavus conidia scarcely synthesized C8 vol...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2014-05-01
|
Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/395.pdf |
id |
doaj-346e2913740448f78b7133190295e4a6 |
---|---|
record_format |
Article |
spelling |
doaj-346e2913740448f78b7133190295e4a62020-11-24T21:54:47ZengPeerJ Inc.PeerJ2167-83592014-05-012e39510.7717/peerj.395395Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germinationKana Miyamoto0Tomoko Murakami1Pattana Kakumyan2Nancy P. Keller3Kenji Matsui4Department of Biological Chemistry, Faculty of Agriculture and the Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Yamaguchi, JapanDepartment of Biological Chemistry, Faculty of Agriculture and the Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Yamaguchi, JapanDepartment of Biological Chemistry, Faculty of Agriculture and the Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Yamaguchi, JapanDepartments of Bacteriology and Medical Microbiology/Immunology, University of Wisconsin-Madison, Madison, WI, USADepartment of Biological Chemistry, Faculty of Agriculture and the Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Yamaguchi, JapanEight-carbon (C8) volatiles, such as 1-octen-3-ol, are ubiquitous among fungi. They are the volatiles critical for aroma and flavor of fungi, and assumed to be signals controlling germination of several fungi. In this study, we found that intact Aspergillus flavus conidia scarcely synthesized C8 volatiles but repeated freeze-thaw treatment that made the cell membrane permeable promoted (R)-1-octen-3-ol formation. Loss or down regulation of any one of five fatty acid oxygenases (PpoA, PpoB, PpoC, PpoD or lipoxygenase) hypothesized contribute to 1-octen-3-ol formation had little impact on production of this volatile. This suggested that none of the oxygenases were directly involved in the formation of 1-octen-3-ol or that compensatory pathways exist in the fungus. Germination of the conidia was markedly inhibited at high density (1.0 × 109spores mL−1). It has been postulated that 1-octen-3-ol is an autoinhibitor suppressing conidia germination at high density. 1-Octen-3-ol at concentration of no less than 10 mM was needed to suppress the germination while the concentration of 1-octen-3-ol in the suspension at 1.0 × 109 mL−1 was under the detection limit (<1 µM). Thus, 1-octen-3-ol was not the principal component responsible for inhibition of germination. Instead, it was evident that the other heat-labile factor(s) suppressed conidial germination.https://peerj.com/articles/395.pdf Aspergillus flavus ConidiaCarbon-eight volatiles1-Octen-3-olFatty acid oxygenasesGermination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kana Miyamoto Tomoko Murakami Pattana Kakumyan Nancy P. Keller Kenji Matsui |
spellingShingle |
Kana Miyamoto Tomoko Murakami Pattana Kakumyan Nancy P. Keller Kenji Matsui Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination PeerJ Aspergillus flavus Conidia Carbon-eight volatiles 1-Octen-3-ol Fatty acid oxygenases Germination |
author_facet |
Kana Miyamoto Tomoko Murakami Pattana Kakumyan Nancy P. Keller Kenji Matsui |
author_sort |
Kana Miyamoto |
title |
Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination |
title_short |
Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination |
title_full |
Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination |
title_fullStr |
Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination |
title_full_unstemmed |
Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination |
title_sort |
formation of 1-octen-3-ol from aspergillus flavus conidia is accelerated after disruption of cells independently of ppo oxygenases, and is not a main cause of inhibition of germination |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2014-05-01 |
description |
Eight-carbon (C8) volatiles, such as 1-octen-3-ol, are ubiquitous among fungi. They are the volatiles critical for aroma and flavor of fungi, and assumed to be signals controlling germination of several fungi. In this study, we found that intact Aspergillus flavus conidia scarcely synthesized C8 volatiles but repeated freeze-thaw treatment that made the cell membrane permeable promoted (R)-1-octen-3-ol formation. Loss or down regulation of any one of five fatty acid oxygenases (PpoA, PpoB, PpoC, PpoD or lipoxygenase) hypothesized contribute to 1-octen-3-ol formation had little impact on production of this volatile. This suggested that none of the oxygenases were directly involved in the formation of 1-octen-3-ol or that compensatory pathways exist in the fungus. Germination of the conidia was markedly inhibited at high density (1.0 × 109spores mL−1). It has been postulated that 1-octen-3-ol is an autoinhibitor suppressing conidia germination at high density. 1-Octen-3-ol at concentration of no less than 10 mM was needed to suppress the germination while the concentration of 1-octen-3-ol in the suspension at 1.0 × 109 mL−1 was under the detection limit (<1 µM). Thus, 1-octen-3-ol was not the principal component responsible for inhibition of germination. Instead, it was evident that the other heat-labile factor(s) suppressed conidial germination. |
topic |
Aspergillus flavus Conidia Carbon-eight volatiles 1-Octen-3-ol Fatty acid oxygenases Germination |
url |
https://peerj.com/articles/395.pdf |
work_keys_str_mv |
AT kanamiyamoto formationof1octen3olfromaspergillusflavusconidiaisacceleratedafterdisruptionofcellsindependentlyofppooxygenasesandisnotamaincauseofinhibitionofgermination AT tomokomurakami formationof1octen3olfromaspergillusflavusconidiaisacceleratedafterdisruptionofcellsindependentlyofppooxygenasesandisnotamaincauseofinhibitionofgermination AT pattanakakumyan formationof1octen3olfromaspergillusflavusconidiaisacceleratedafterdisruptionofcellsindependentlyofppooxygenasesandisnotamaincauseofinhibitionofgermination AT nancypkeller formationof1octen3olfromaspergillusflavusconidiaisacceleratedafterdisruptionofcellsindependentlyofppooxygenasesandisnotamaincauseofinhibitionofgermination AT kenjimatsui formationof1octen3olfromaspergillusflavusconidiaisacceleratedafterdisruptionofcellsindependentlyofppooxygenasesandisnotamaincauseofinhibitionofgermination |
_version_ |
1725865809233838080 |