Nano-engineered microcapsules boost the treatment of persistent pain
Persistent pain remains a major health issue: common treatments relying on either repeated local injections or systemic drug administration are prone to concomitant side-effects. It is thought that an alternative could be a multifunctional cargo system to deliver medicine to the target site and rele...
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2018-01-01
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Online Access: | http://dx.doi.org/10.1080/10717544.2018.1431981 |
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doaj-3472fd2454674001bda63f95f79481062020-11-25T02:38:17ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642018-01-0125143544710.1080/10717544.2018.14319811431981Nano-engineered microcapsules boost the treatment of persistent painOlga Kopach0Kayiu Zheng1Luo Dong2Andrei Sapelkin3Nana Voitenko4Gleb B. Sukhorukov5Dmitri A. Rusakov6University College LondonUniversity College LondonQueen Mary University of LondonQueen Mary University of LondonBogomoletz Institute of PhysiologyQueen Mary University of LondonUniversity College LondonPersistent pain remains a major health issue: common treatments relying on either repeated local injections or systemic drug administration are prone to concomitant side-effects. It is thought that an alternative could be a multifunctional cargo system to deliver medicine to the target site and release it over a prolonged time window. We nano-engineered microcapsules equipped with adjustable cargo release properties and encapsulated the sodium-channel blocker QX-314 using the layer-by-layer (LbL) technology. First, we employed single-cell electrophysiology to establish in vitro that microcapsule application can dampen neuronal excitability in a controlled fashion. Secondly, we used two-photon excitation imaging to monitor and adjust long-lasting release of encapsulated cargo in target tissue in situ. Finally, we explored an established peripheral inflammation model in rodents to find that a single local injection of QX-314-containing microcapsules could provide robust pain relief lasting for over a week. This was accompanied by a recovery of the locomotive deficit and the amelioration of anxiety in animals with persistent inflammation. Post hoc immunohistology confirmed biodegradation of microcapsules over a period of several weeks. The overall remedial effect lasted 10–20 times longer than that of a single focal drug injection. It depended on the QX-314 encapsulation levels, involved TRPV1-channel-dependent cell permeability of QX-314, and showed no detectable side-effects. Our data suggest that nano-engineered encapsulation provides local drug delivery suitable for prolonged pain relief, which could be highly advantageous compared to existing treatments.http://dx.doi.org/10.1080/10717544.2018.1431981biodegradable microcapsulespersistent painna+ channelsdrug diffusionneuronal excitabilitypain relieflocomotive deficit and anxiety |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olga Kopach Kayiu Zheng Luo Dong Andrei Sapelkin Nana Voitenko Gleb B. Sukhorukov Dmitri A. Rusakov |
spellingShingle |
Olga Kopach Kayiu Zheng Luo Dong Andrei Sapelkin Nana Voitenko Gleb B. Sukhorukov Dmitri A. Rusakov Nano-engineered microcapsules boost the treatment of persistent pain Drug Delivery biodegradable microcapsules persistent pain na+ channels drug diffusion neuronal excitability pain relief locomotive deficit and anxiety |
author_facet |
Olga Kopach Kayiu Zheng Luo Dong Andrei Sapelkin Nana Voitenko Gleb B. Sukhorukov Dmitri A. Rusakov |
author_sort |
Olga Kopach |
title |
Nano-engineered microcapsules boost the treatment of persistent pain |
title_short |
Nano-engineered microcapsules boost the treatment of persistent pain |
title_full |
Nano-engineered microcapsules boost the treatment of persistent pain |
title_fullStr |
Nano-engineered microcapsules boost the treatment of persistent pain |
title_full_unstemmed |
Nano-engineered microcapsules boost the treatment of persistent pain |
title_sort |
nano-engineered microcapsules boost the treatment of persistent pain |
publisher |
Taylor & Francis Group |
series |
Drug Delivery |
issn |
1071-7544 1521-0464 |
publishDate |
2018-01-01 |
description |
Persistent pain remains a major health issue: common treatments relying on either repeated local injections or systemic drug administration are prone to concomitant side-effects. It is thought that an alternative could be a multifunctional cargo system to deliver medicine to the target site and release it over a prolonged time window. We nano-engineered microcapsules equipped with adjustable cargo release properties and encapsulated the sodium-channel blocker QX-314 using the layer-by-layer (LbL) technology. First, we employed single-cell electrophysiology to establish in vitro that microcapsule application can dampen neuronal excitability in a controlled fashion. Secondly, we used two-photon excitation imaging to monitor and adjust long-lasting release of encapsulated cargo in target tissue in situ. Finally, we explored an established peripheral inflammation model in rodents to find that a single local injection of QX-314-containing microcapsules could provide robust pain relief lasting for over a week. This was accompanied by a recovery of the locomotive deficit and the amelioration of anxiety in animals with persistent inflammation. Post hoc immunohistology confirmed biodegradation of microcapsules over a period of several weeks. The overall remedial effect lasted 10–20 times longer than that of a single focal drug injection. It depended on the QX-314 encapsulation levels, involved TRPV1-channel-dependent cell permeability of QX-314, and showed no detectable side-effects. Our data suggest that nano-engineered encapsulation provides local drug delivery suitable for prolonged pain relief, which could be highly advantageous compared to existing treatments. |
topic |
biodegradable microcapsules persistent pain na+ channels drug diffusion neuronal excitability pain relief locomotive deficit and anxiety |
url |
http://dx.doi.org/10.1080/10717544.2018.1431981 |
work_keys_str_mv |
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