Kinetic Model For Triglyceride Hydrolysis Using Lipase:Review

• Main Authors: Heri Hermansyah, Anondho Wijanarko, Dianursanti Dianursanti, Misri Gozan, Praswasti P. D.K Wulan, Rita Arbianti, Roekmijati W. Soemantojo, Tania Surya Utami, Yuliusman Yuliusman, Momoji Kubo, Naomi Shibasaki-Kitakawa, Toshiy Yonemoto
• Format: Article
• Language: English
• Published: Universitas Indonesia 2010-10-01
• Series: Makara Journal of Technology
• Subjects: lipase, kinetic model, enzymatic reaction mechanism, hydrolysis, triglyceride
• Online Access: http://journal.ui.ac.id/technology/journal/article/view/124
• ISSN: 2355-2786; 2356-4539

Triglyceride hydrolysis using lipase has been proposed as a novel method to produce raw materials in food and cosmetic industries such as diacylglycerol, monoacylglycerol, glycerol and fatty acid. In order to design a reactor for utilizing this reaction on industrial scale, constructing a kinetic model is important. Since the substrates are oil and water, the hydrolysis takes place at oil-water interface. Furthermore, the triglyceride has three ester bonds, so that the hydrolysis stepwise proceeds. Thus, the reaction mechanism is very complicated. The difference between the interfacial and bulk concentrations of the enzyme, substrates and products, and the interfacial enzymatic reaction mechanism should be considered in the model. <br>