PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION
The present work aimed to study the impact of infection with a locally isolated vNDV genotype VII in commercial broiler chickens of different ages from a histopathological point of view with related changes in pulmonary cytokine expression. 100 commercial broiler chickens were allocated into 4 gro...
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West Bengal Veterinary Alumni Association
2021-09-01
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doaj-3476e9c0392248da9c2d00cb580132bd2021-09-18T15:08:51ZengWest Bengal Veterinary Alumni AssociationExploratory Animal and Medical Research2277-470X2319-247X2021-09-01111677910.52635/eamr/11.1.67-79PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSIONMohamed R. Mousa0Faten F. Mohammed 1 Ayman H. El-Deeb2Fatma Amer3Kawkab A. Ahmed4Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Giza, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.The present work aimed to study the impact of infection with a locally isolated vNDV genotype VII in commercial broiler chickens of different ages from a histopathological point of view with related changes in pulmonary cytokine expression. 100 commercial broiler chickens were allocated into 4 groups as follows; group 1 served as control non-infected group and three infected groups, were infected with 106 EID50 NDV at ages of 10,20, and 30 days. Tissue samples from different organs were collected at the 1st, 3rd, 5th, and 7th days post-inoculation for histopathological and immunohistochemical examination. Quantitative real-time RT-PCR for virus re-isolation as well as expression of IL-6 and IFN-γ genes estimation were performed on lung tissue collected at 3 dpi. RT-PCR for virus revealed increased virus load in the lungs of older birds and cytokines profiling revealed significant up-regulation of both IL-6 and IFN-γ genes in the groups that received infection at older ages. The severity and distribution of pathological lesions were directly proportioned to the age of infection and the time interval post-infection. Immunostaining of the viral antigen in the different tissues confirmed the pantropic nature of the virus.http://www.animalmedicalresearch.org/Vol.11_Issue-1_June_2021/PATHOLOGICAL%20MONITORING.pdfvelogenic new castle disease virusil-6ifn-γpathologyimmunohistochemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed R. Mousa Faten F. Mohammed Ayman H. El-Deeb Fatma Amer Kawkab A. Ahmed |
spellingShingle |
Mohamed R. Mousa Faten F. Mohammed Ayman H. El-Deeb Fatma Amer Kawkab A. Ahmed PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION Exploratory Animal and Medical Research velogenic new castle disease virus il-6 ifn-γ pathology immunohistochemistry |
author_facet |
Mohamed R. Mousa Faten F. Mohammed Ayman H. El-Deeb Fatma Amer Kawkab A. Ahmed |
author_sort |
Mohamed R. Mousa |
title |
PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION |
title_short |
PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION |
title_full |
PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION |
title_fullStr |
PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION |
title_full_unstemmed |
PATHOLOGICAL MONITORING OF VISCERAL LESIONS INDUCED BY VELOGENIC NEWCASTLE DISEASE IN EXPERIMENTALLY INFECTED BROILER CHICKENS AT DIFFERENT AGES WITH SPECIAL REFERENCE TO PULMONARY INFLAMMATORY CYTOKINES EXPRESSION |
title_sort |
pathological monitoring of visceral lesions induced by velogenic newcastle disease in experimentally infected broiler chickens at different ages with special reference to pulmonary inflammatory cytokines expression |
publisher |
West Bengal Veterinary Alumni Association |
series |
Exploratory Animal and Medical Research |
issn |
2277-470X 2319-247X |
publishDate |
2021-09-01 |
description |
The present work aimed to study the impact of infection with a locally isolated vNDV genotype VII in
commercial broiler chickens of different ages from a histopathological point of view with related changes in pulmonary
cytokine expression. 100 commercial broiler chickens were allocated into 4 groups as follows; group 1 served as control
non-infected group and three infected groups, were infected with 106 EID50 NDV at ages of 10,20, and 30 days. Tissue
samples from different organs were collected at the 1st, 3rd, 5th, and 7th days post-inoculation for histopathological and
immunohistochemical examination. Quantitative real-time RT-PCR for virus re-isolation as well as expression of IL-6
and IFN-γ genes estimation were performed on lung tissue collected at 3 dpi. RT-PCR for virus revealed increased virus
load in the lungs of older birds and cytokines profiling revealed significant up-regulation of both IL-6 and IFN-γ genes in
the groups that received infection at older ages. The severity and distribution of pathological lesions were directly
proportioned to the age of infection and the time interval post-infection. Immunostaining of the viral antigen in the
different tissues confirmed the pantropic nature of the virus. |
topic |
velogenic new castle disease virus il-6 ifn-γ pathology immunohistochemistry |
url |
http://www.animalmedicalresearch.org/Vol.11_Issue-1_June_2021/PATHOLOGICAL%20MONITORING.pdf |
work_keys_str_mv |
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