Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity

Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity

The Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids from Taraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol...

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Main Authors: Zongxi Sun, Ruiqiang Su, Jianwei Qiao, Zhiquan Zhao, Xinsheng Wang
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2014/956278
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spelling doaj-347d319938884e9bb6f8df56ebfa72412020-11-25T00:53:51ZengHindawi LimitedJournal of Chemistry2090-90632090-90712014-01-01201410.1155/2014/956278956278Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant ActivityZongxi Sun0Ruiqiang Su1Jianwei Qiao2Zhiquan Zhao3Xinsheng Wang4State Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, ChinaState Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, ChinaState Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, ChinaState Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, ChinaState Key Laboratory of Generic Pharmaceutical Technology for Traditional Chinese Medicine, Linyi 276006, ChinaThe Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids from Taraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5 : 1 (mL/g) liquid-solid ratio for 43.8 min. The crude extract was then purified by HPD-100 macroporous adsorption resin, and the flavonoids content in the purified extract increased to 54.7%. The antioxidant activity of the purified flavonoids was evaluated in vitro by scavenging capacity of ABTS or DPPH, β-carotene bleaching, and FTC test. The knowledge obtained from this study should be useful to further develop and apply this plant resource.http://dx.doi.org/10.1155/2014/956278
collection DOAJ
language English
format Article
sources DOAJ
author Zongxi Sun
Ruiqiang Su
Jianwei Qiao
Zhiquan Zhao
Xinsheng Wang
spellingShingle Zongxi Sun
Ruiqiang Su
Jianwei Qiao
Zhiquan Zhao
Xinsheng Wang
Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
Journal of Chemistry
author_facet Zongxi Sun
Ruiqiang Su
Jianwei Qiao
Zhiquan Zhao
Xinsheng Wang
author_sort Zongxi Sun
title Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
title_short Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
title_full Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
title_fullStr Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
title_full_unstemmed Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity
title_sort flavonoids extraction from taraxacum officinale (dandelion): optimisation using response surface methodology and antioxidant activity
publisher Hindawi Limited
series Journal of Chemistry
issn 2090-9063
2090-9071
publishDate 2014-01-01
description The Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids from Taraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5 : 1 (mL/g) liquid-solid ratio for 43.8 min. The crude extract was then purified by HPD-100 macroporous adsorption resin, and the flavonoids content in the purified extract increased to 54.7%. The antioxidant activity of the purified flavonoids was evaluated in vitro by scavenging capacity of ABTS or DPPH, β-carotene bleaching, and FTC test. The knowledge obtained from this study should be useful to further develop and apply this plant resource.
url http://dx.doi.org/10.1155/2014/956278
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AT ruiqiangsu flavonoidsextractionfromtaraxacumofficinaledandelionoptimisationusingresponsesurfacemethodologyandantioxidantactivity
AT jianweiqiao flavonoidsextractionfromtaraxacumofficinaledandelionoptimisationusingresponsesurfacemethodologyandantioxidantactivity
AT zhiquanzhao flavonoidsextractionfromtaraxacumofficinaledandelionoptimisationusingresponsesurfacemethodologyandantioxidantactivity
AT xinshengwang flavonoidsextractionfromtaraxacumofficinaledandelionoptimisationusingresponsesurfacemethodologyandantioxidantactivity
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