Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt
Bovine papillomatosis is a viral disease of cattle causing cutaneous warts. A diagnosis of this viral infection is very mandatory for combating the resulting economic losses. Given the limited data available about bovine papillomavirus (BPV) in Egypt, the present study involved the molecular diagnos...
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doaj-347e00c26ab348c2a2853eb8052965b62020-11-25T03:57:44ZengMDPI AGAnimals2076-26152020-10-01101929192910.3390/ani10101929Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from EgyptMohamed El-Tholoth0Michael G. Mauk1Yasser F. Elnaker2Samah M. Mosad3Amin Tahoun4Mohamed W. El-Sherif5Maha S. Lokman6Rami B. Kassab7Ahmed Abdelsadik8Ayman A. Saleh9Ehab Kotb Elmahallawy10Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USADepartment of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, The New Valley University, El-Karga 72511, New Valley, EgyptDepartment of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Animal Medicine, Faculty of Veterinary Medicine, Kafrelshkh University, Kafrelsheikh 33511, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, The New Valley University, El-Karga 72511, New Valley, EgyptBiology Department, College of Science and Humanities, Prince Sattam bin Abdul Aziz University, Alkharj 11942, Saudi ArabiaDepartment of Zoology and Entomology, Faculty of Science, Helwan University, 11795 Cairo, EgyptZoology Department, Faculty of Science, Aswan University, Aswan 81528, EgyptDepartment of Animal Wealth Development, Genetics and Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Biomedical Sciences, University of Leon, 24071 León, SpainBovine papillomatosis is a viral disease of cattle causing cutaneous warts. A diagnosis of this viral infection is very mandatory for combating the resulting economic losses. Given the limited data available about bovine papillomavirus (BPV) in Egypt, the present study involved the molecular diagnosis of bovine papillomavirus type-1 (BPV-1), -2, -4, -5, and -10 in cattle presenting cutaneous warts on the head and neck from New Valley Province, Egypt. The phylogenetic analysis of the detected types of BPV was also performed, followed by developing a point-of-need molecular assay for the rapid identification of identified BPV types. In this regard, a total of 308 cattle from private farms in Egypt were clinically examined, of which 13 animals presented cutaneous warts due to suspected BPV infection. The symptomatic animals were treated surgically, and biopsies from skin lesions were collected for BPV-1, -2, -4, -5, and -10 molecular identification using polymerase chain reaction (PCR). The presence of BPV-1 DNA was confirmed in 11 collected samples (84.6%), while BPV-2, -4, -5, and -10 were not detected. Sequencing of the PCR products suggested the Egyptian virus is closely related to BPV found in India. An isothermal nucleic acid amplification test (NAAT) with labeled primers specific for the BPV-1 L1 gene sequence, and based on recombinase polymerase amplification (RPA), in combination with a lateral flow strip assay for the detection of RPA products, was developed and tested. The point-of-need molecular assay demonstrated a diagnostic utility comparable to PCR-based testing. Taken together, the present study provides interesting molecular data related to the occurrence of BPV-1 in Egypt and reveals the genetic relatedness of the Egyptian BPV-1 with BPV-1 found in buffalo in India. In addition, a simple, low-cost combined test was also validated for diagnosis of the infection. The present study suggests the necessity of future investigations about the circulating strains of the virus among the cattle in Egypt to assess their genetic relatedness and better understand the epidemiological pattern of the disease.https://www.mdpi.com/2076-2615/10/10/1929bovine papillomaviruscattleEgyptnucleic acid lateral flow immunoassayPCR |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed El-Tholoth Michael G. Mauk Yasser F. Elnaker Samah M. Mosad Amin Tahoun Mohamed W. El-Sherif Maha S. Lokman Rami B. Kassab Ahmed Abdelsadik Ayman A. Saleh Ehab Kotb Elmahallawy |
spellingShingle |
Mohamed El-Tholoth Michael G. Mauk Yasser F. Elnaker Samah M. Mosad Amin Tahoun Mohamed W. El-Sherif Maha S. Lokman Rami B. Kassab Ahmed Abdelsadik Ayman A. Saleh Ehab Kotb Elmahallawy Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt Animals bovine papillomavirus cattle Egypt nucleic acid lateral flow immunoassay PCR |
author_facet |
Mohamed El-Tholoth Michael G. Mauk Yasser F. Elnaker Samah M. Mosad Amin Tahoun Mohamed W. El-Sherif Maha S. Lokman Rami B. Kassab Ahmed Abdelsadik Ayman A. Saleh Ehab Kotb Elmahallawy |
author_sort |
Mohamed El-Tholoth |
title |
Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt |
title_short |
Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt |
title_full |
Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt |
title_fullStr |
Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt |
title_full_unstemmed |
Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt |
title_sort |
molecular characterization and developing a point-of-need molecular test for diagnosis of bovine papillomavirus (bpv) type 1 in cattle from egypt |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2020-10-01 |
description |
Bovine papillomatosis is a viral disease of cattle causing cutaneous warts. A diagnosis of this viral infection is very mandatory for combating the resulting economic losses. Given the limited data available about bovine papillomavirus (BPV) in Egypt, the present study involved the molecular diagnosis of bovine papillomavirus type-1 (BPV-1), -2, -4, -5, and -10 in cattle presenting cutaneous warts on the head and neck from New Valley Province, Egypt. The phylogenetic analysis of the detected types of BPV was also performed, followed by developing a point-of-need molecular assay for the rapid identification of identified BPV types. In this regard, a total of 308 cattle from private farms in Egypt were clinically examined, of which 13 animals presented cutaneous warts due to suspected BPV infection. The symptomatic animals were treated surgically, and biopsies from skin lesions were collected for BPV-1, -2, -4, -5, and -10 molecular identification using polymerase chain reaction (PCR). The presence of BPV-1 DNA was confirmed in 11 collected samples (84.6%), while BPV-2, -4, -5, and -10 were not detected. Sequencing of the PCR products suggested the Egyptian virus is closely related to BPV found in India. An isothermal nucleic acid amplification test (NAAT) with labeled primers specific for the BPV-1 L1 gene sequence, and based on recombinase polymerase amplification (RPA), in combination with a lateral flow strip assay for the detection of RPA products, was developed and tested. The point-of-need molecular assay demonstrated a diagnostic utility comparable to PCR-based testing. Taken together, the present study provides interesting molecular data related to the occurrence of BPV-1 in Egypt and reveals the genetic relatedness of the Egyptian BPV-1 with BPV-1 found in buffalo in India. In addition, a simple, low-cost combined test was also validated for diagnosis of the infection. The present study suggests the necessity of future investigations about the circulating strains of the virus among the cattle in Egypt to assess their genetic relatedness and better understand the epidemiological pattern of the disease. |
topic |
bovine papillomavirus cattle Egypt nucleic acid lateral flow immunoassay PCR |
url |
https://www.mdpi.com/2076-2615/10/10/1929 |
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