Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G<sub>1</sub>Phy), and UV irradiation of methacrylate were developed, characterized...
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doaj-348566e804804e1d814b7e7c761938342020-11-25T04:10:33ZengMDPI AGPolymers2073-43602020-11-01122661266110.3390/polym12112661Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue EngineeringAna Mora-Boza0Elena López-Ruiz1María Luisa López-Donaire2Gema Jiménez3María Rosa Aguilar4Juan Antonio Marchal5José Luis Pedraz6Blanca Vázquez-Lasa7Julio San Román8Patricia Gálvez-Martín9Institute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, E-18100 Granada, SpainInstitute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, E-18100 Granada, SpainInstitute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, E-18100 Granada, SpainCIBER-BBN, Health Institute Carlos III, C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, SpainInstitute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainInstitute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainR&D Human Health, Bioibérica S.A.U., 08950 Barcelona, SpainIn the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G<sub>1</sub>Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.https://www.mdpi.com/2073-4360/12/11/2661interpenetrated polymer networksemi-IPNmethacrylated hyaluronic acidchitosanglycerylphytatemesenchymal stem cell |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Mora-Boza Elena López-Ruiz María Luisa López-Donaire Gema Jiménez María Rosa Aguilar Juan Antonio Marchal José Luis Pedraz Blanca Vázquez-Lasa Julio San Román Patricia Gálvez-Martín |
spellingShingle |
Ana Mora-Boza Elena López-Ruiz María Luisa López-Donaire Gema Jiménez María Rosa Aguilar Juan Antonio Marchal José Luis Pedraz Blanca Vázquez-Lasa Julio San Román Patricia Gálvez-Martín Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering Polymers interpenetrated polymer network semi-IPN methacrylated hyaluronic acid chitosan glycerylphytate mesenchymal stem cell |
author_facet |
Ana Mora-Boza Elena López-Ruiz María Luisa López-Donaire Gema Jiménez María Rosa Aguilar Juan Antonio Marchal José Luis Pedraz Blanca Vázquez-Lasa Julio San Román Patricia Gálvez-Martín |
author_sort |
Ana Mora-Boza |
title |
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering |
title_short |
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering |
title_full |
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering |
title_fullStr |
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering |
title_full_unstemmed |
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering |
title_sort |
evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2020-11-01 |
description |
In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G<sub>1</sub>Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field. |
topic |
interpenetrated polymer network semi-IPN methacrylated hyaluronic acid chitosan glycerylphytate mesenchymal stem cell |
url |
https://www.mdpi.com/2073-4360/12/11/2661 |
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