Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However,...
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doaj-349801c61dfc4631b86766a558cd56c42020-11-24T21:43:42ZengElsevierBone Reports2352-18722017-06-016C646910.1016/j.bonr.2017.02.002Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitroN.R. Alqhtani0N.J. Logan1S. Meghji2R. Leeson3P.M. Brett4University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKSince the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).http://www.sciencedirect.com/science/article/pii/S2352187217300062Bone remodelling/regenerationHuman mesenchymal stem cellsBisphosphonatesTitanium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N.R. Alqhtani N.J. Logan S. Meghji R. Leeson P.M. Brett |
spellingShingle |
N.R. Alqhtani N.J. Logan S. Meghji R. Leeson P.M. Brett Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro Bone Reports Bone remodelling/regeneration Human mesenchymal stem cells Bisphosphonates Titanium |
author_facet |
N.R. Alqhtani N.J. Logan S. Meghji R. Leeson P.M. Brett |
author_sort |
N.R. Alqhtani |
title |
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro |
title_short |
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro |
title_full |
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro |
title_fullStr |
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro |
title_full_unstemmed |
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro |
title_sort |
low dose effect of bisphosphonates on hmscs osteogenic response to titanium surface in vitro |
publisher |
Elsevier |
series |
Bone Reports |
issn |
2352-1872 |
publishDate |
2017-06-01 |
description |
Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05). |
topic |
Bone remodelling/regeneration Human mesenchymal stem cells Bisphosphonates Titanium |
url |
http://www.sciencedirect.com/science/article/pii/S2352187217300062 |
work_keys_str_mv |
AT nralqhtani lowdoseeffectofbisphosphonatesonhmscsosteogenicresponsetotitaniumsurfaceinvitro AT njlogan lowdoseeffectofbisphosphonatesonhmscsosteogenicresponsetotitaniumsurfaceinvitro AT smeghji lowdoseeffectofbisphosphonatesonhmscsosteogenicresponsetotitaniumsurfaceinvitro AT rleeson lowdoseeffectofbisphosphonatesonhmscsosteogenicresponsetotitaniumsurfaceinvitro AT pmbrett lowdoseeffectofbisphosphonatesonhmscsosteogenicresponsetotitaniumsurfaceinvitro |
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1725912445840523264 |