Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro

Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However,...

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Main Authors: N.R. Alqhtani, N.J. Logan, S. Meghji, R. Leeson, P.M. Brett
Format: Article
Language:English
Published: Elsevier 2017-06-01
Series:Bone Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352187217300062
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spelling doaj-349801c61dfc4631b86766a558cd56c42020-11-24T21:43:42ZengElsevierBone Reports2352-18722017-06-016C646910.1016/j.bonr.2017.02.002Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitroN.R. Alqhtani0N.J. Logan1S. Meghji2R. Leeson3P.M. Brett4University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKUniversity College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKSince the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).http://www.sciencedirect.com/science/article/pii/S2352187217300062Bone remodelling/regenerationHuman mesenchymal stem cellsBisphosphonatesTitanium
collection DOAJ
language English
format Article
sources DOAJ
author N.R. Alqhtani
N.J. Logan
S. Meghji
R. Leeson
P.M. Brett
spellingShingle N.R. Alqhtani
N.J. Logan
S. Meghji
R. Leeson
P.M. Brett
Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
Bone Reports
Bone remodelling/regeneration
Human mesenchymal stem cells
Bisphosphonates
Titanium
author_facet N.R. Alqhtani
N.J. Logan
S. Meghji
R. Leeson
P.M. Brett
author_sort N.R. Alqhtani
title Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
title_short Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
title_full Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
title_fullStr Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
title_full_unstemmed Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro
title_sort low dose effect of bisphosphonates on hmscs osteogenic response to titanium surface in vitro
publisher Elsevier
series Bone Reports
issn 2352-1872
publishDate 2017-06-01
description Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).
topic Bone remodelling/regeneration
Human mesenchymal stem cells
Bisphosphonates
Titanium
url http://www.sciencedirect.com/science/article/pii/S2352187217300062
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