A breakthrough in protein engineering of a glycosyltransferase

A breakthrough in protein engineering of a glycosyltransferase

An intriguing advance in the rational protein engineering of a newly identified glycosyltransferase from Siraitia grosvenorii (UGT74AC2) has been accomplished by Zhoutong Sun, Yuanxia Sun and coworkers for the targeted pharmaceutically significant glucosylation of the polyhydroxy substrate silybin....

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Bibliographic Details
Main Author: Manfred T. Reetz
Format: Article
Language:English
Published: KeAi Communications Co. Ltd. 2021-02-01
Series:Green Synthesis and Catalysis
Subjects:
Rational design
FRISM
Regioselectivity
Glycosyltransferase
Online Access:http://www.sciencedirect.com/science/article/pii/S2666554921000077
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spelling doaj-349b368b2ccc44b587d90d4b133f8bc12021-10-05T04:21:36ZengKeAi Communications Co. Ltd.Green Synthesis and Catalysis2666-55492021-02-012145A breakthrough in protein engineering of a glycosyltransferaseManfred T. Reetz0Max-Planck-Institut Fuer Kohlenforschung, Kaiser-Wilhelm-Platz 1, Muelheim 45470, Germany; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Max-Planck-Institut fuer Kohlenforschung, Kaiser-Wilhelm-Platz 1, Muelheim 45470, Germany.An intriguing advance in the rational protein engineering of a newly identified glycosyltransferase from Siraitia grosvenorii (UGT74AC2) has been accomplished by Zhoutong Sun, Yuanxia Sun and coworkers for the targeted pharmaceutically significant glucosylation of the polyhydroxy substrate silybin. Using an advanced version of Focused Rational Iterative Site-specific Mutagenesis (FRISM), they were able to evolve mutants that allow the formation of the 3-OH, 7-OH and the 3,7-O-diglycoside products with 94%, > 99% and > 99% regioselectivity, respectively. This can be viewed as a breakthrough because the wildtype UGT74AC2 delivers a difficult to separate mixture of products in a ratio of 22%:39%:39%. Even N- and S-glucosylations were achieved, a likewise unusual accomplishment.http://www.sciencedirect.com/science/article/pii/S2666554921000077Rational designFRISMRegioselectivityGlycosyltransferase
collection DOAJ
language English
format Article
sources DOAJ
author Manfred T. Reetz
spellingShingle Manfred T. Reetz
A breakthrough in protein engineering of a glycosyltransferase
Green Synthesis and Catalysis
Rational design
FRISM
Regioselectivity
Glycosyltransferase
author_facet Manfred T. Reetz
author_sort Manfred T. Reetz
title A breakthrough in protein engineering of a glycosyltransferase
title_short A breakthrough in protein engineering of a glycosyltransferase
title_full A breakthrough in protein engineering of a glycosyltransferase
title_fullStr A breakthrough in protein engineering of a glycosyltransferase
title_full_unstemmed A breakthrough in protein engineering of a glycosyltransferase
title_sort breakthrough in protein engineering of a glycosyltransferase
publisher KeAi Communications Co. Ltd.
series Green Synthesis and Catalysis
issn 2666-5549
publishDate 2021-02-01
description An intriguing advance in the rational protein engineering of a newly identified glycosyltransferase from Siraitia grosvenorii (UGT74AC2) has been accomplished by Zhoutong Sun, Yuanxia Sun and coworkers for the targeted pharmaceutically significant glucosylation of the polyhydroxy substrate silybin. Using an advanced version of Focused Rational Iterative Site-specific Mutagenesis (FRISM), they were able to evolve mutants that allow the formation of the 3-OH, 7-OH and the 3,7-O-diglycoside products with 94%, > 99% and > 99% regioselectivity, respectively. This can be viewed as a breakthrough because the wildtype UGT74AC2 delivers a difficult to separate mixture of products in a ratio of 22%:39%:39%. Even N- and S-glucosylations were achieved, a likewise unusual accomplishment.
topic Rational design
FRISM
Regioselectivity
Glycosyltransferase
url http://www.sciencedirect.com/science/article/pii/S2666554921000077
work_keys_str_mv AT manfredtreetz abreakthroughinproteinengineeringofaglycosyltransferase
AT manfredtreetz breakthroughinproteinengineeringofaglycosyltransferase
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