Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique Estudo comparativo da obtenção de hepatócitos humanos pela técnica ácido etilenodiaminotetracético e da colegenase

Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique Estudo comparativo da obtenção de hepatócitos humanos pela técnica ácido etilenodiaminotetracético e da colegenase

PURPOSE: To compare the viability of human hepatocytes dissociated by the ethylenediaminetetraacetic acid and collagenase techniques. METHODS: Hepatocytes were prepared by dissociation of liver fragments obtained from hepatectomies performed for therapeutic purposes at the Service of Digestive Tract...

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Bibliographic Details
Main Authors: Eduardo Crema, Ana Márcia Werneck, Aiodair Martins Júnior, Lara Beatriz Prata Ribeiro, Tatiana Silva de Lima, Alex Augusto Silva
Format: Article
Language:English
Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2007-02-01
Series:Acta Cirurgica Brasileira
Subjects:
Separação Celular
Transplante de Células
Ácido Edético
Cell Separation
Cell Transplantation
Edetic Acid
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000100010

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