Structural Analysis of Fast-Growing Aspen Alkaline Peroxide Mechanical Pulp Lignin: A Post-Enzymatic Treatment
An enzymatic mild acidic hydrolysis was used to separate and purify residual lignin from alkaline peroxide mechanical pulp (APMP). Using the optimum conditions for the laccase treatment (pH 4.5, temperature 50 °C, lignin consistency of 1%, a reaction time of 60 min, and a laccase dosage of 8 μ/g), o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2016-02-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2723_Wang_Structural_Analysis_Fast_Growing_Aspen |
| Summary: | An enzymatic mild acidic hydrolysis was used to separate and purify residual lignin from alkaline peroxide mechanical pulp (APMP). Using the optimum conditions for the laccase treatment (pH 4.5, temperature 50 °C, lignin consistency of 1%, a reaction time of 60 min, and a laccase dosage of 8 μ/g), oven-dried lignin was treated with laccase and in a laccase mediator system (LMS) to explore the mechanism for laccase and the LMS modification of APMP. The changes of functional groups in lignin were analyzed using nuclear magnetic resonance (31P-NMR and 13C-NMR). The molecular weight distributions of the lignin samples were confirmed by gel permeation chromatography (GPC). The 31P-NMR and 13C-NMR spectra revealed that the lignin structure changed significantly with the laccase and the LMS treatments. Meanwhile, GPC demonstrated that laccase without a mediator could lead to the polymerization of lignin, while the LMS could degrade the lignin. Hence, it was concluded that laccase is an attractive enzyme for lignin modification. |
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| ISSN: | 1930-2126 1930-2126 |