EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA
Elephantopus scaber Linn. has been reported to have cytotoxic effects on breast cancer cells and the potential to be developed as anticancer agent. This study aims was to determine the cytotoxic activity and apoptosis induction effect of petroleum ether fractions of ethanolic extract of (Elephanto...
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doaj-34c4ef93a3da4bd3bc6e010cbc803f3c2021-06-02T14:08:22ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562013-11-01321710.12928/pharmaciana.v3i2.424EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELAYeni Listyowati 0Nurkhasanah Nurkhasanah1Universitas Ahmad DahlanUniversitas Ahmad DahlanElephantopus scaber Linn. has been reported to have cytotoxic effects on breast cancer cells and the potential to be developed as anticancer agent. This study aims was to determine the cytotoxic activity and apoptosis induction effect of petroleum ether fractions of ethanolic extract of (Elephantopus scaber Linn) leaves against cervical cancer cells (HeLa). Petroleum ether fraction was obtained by dissolving eyhanolic extract in petroleum ether, and the soluble fraction was as petroleum ether fraction. The method used for cytotoxic activity test was MTT test. The concentration series used were 2000; 1500; 1000; 800; 400; 200; 100; 50; 25; 12.5; 6.25 and 3.125 mg/ml. The IC50 used as cytotoxic parameters. The apoptotic observations was conducted using acridine orange and ethidium bromide. The study showed the IC50 of petroleum ether fraction of (Elephantopus scaber Linn) ethanolic extract was 185 ug/ml. The study also showed the potency to stimulate apoptosis in HeLa cells.http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/424/279Elephantopus scaber LinncytotoxicityapoptosisHeLa cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yeni Listyowati Nurkhasanah Nurkhasanah |
spellingShingle |
Yeni Listyowati Nurkhasanah Nurkhasanah EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA Pharmaciana Elephantopus scaber Linn cytotoxicity apoptosis HeLa cells |
author_facet |
Yeni Listyowati Nurkhasanah Nurkhasanah |
author_sort |
Yeni Listyowati |
title |
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA |
title_short |
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA |
title_full |
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA |
title_fullStr |
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA |
title_full_unstemmed |
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA |
title_sort |
efek sitotoksik dan pemacuan apoptosis fraksi petroleum eter ekstrak etanol daun tapak liman (elephantopus scaber linn) terhadap sel hela |
publisher |
Universitas Ahmad Dahlan |
series |
Pharmaciana |
issn |
2088-4559 2477-0256 |
publishDate |
2013-11-01 |
description |
Elephantopus scaber Linn. has been reported to have cytotoxic effects on breast cancer cells and the
potential to be developed as anticancer agent. This study aims was to determine the cytotoxic activity and
apoptosis induction effect of petroleum ether fractions of ethanolic extract of (Elephantopus scaber Linn) leaves
against cervical cancer cells (HeLa). Petroleum ether fraction was obtained by dissolving eyhanolic extract in
petroleum ether, and the soluble fraction was as petroleum ether fraction. The method used for cytotoxic activity
test was MTT test. The concentration series used were 2000; 1500; 1000; 800; 400; 200; 100; 50; 25; 12.5; 6.25
and 3.125 mg/ml. The IC50 used as cytotoxic parameters. The apoptotic observations was conducted using
acridine orange and ethidium bromide. The study showed the IC50 of petroleum ether fraction of (Elephantopus
scaber Linn) ethanolic extract was 185 ug/ml. The study also showed the potency to stimulate apoptosis in HeLa
cells. |
topic |
Elephantopus scaber Linn cytotoxicity apoptosis HeLa cells |
url |
http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/424/279 |
work_keys_str_mv |
AT yenilistyowati efeksitotoksikdanpemacuanapoptosisfraksipetroleumeterekstraketanoldauntapaklimanelephantopusscaberlinnterhadapselhela AT nurkhasanahnurkhasanah efeksitotoksikdanpemacuanapoptosisfraksipetroleumeterekstraketanoldauntapaklimanelephantopusscaberlinnterhadapselhela |
_version_ |
1721403720122499072 |