D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence
The use of inositols in endocrinological clinical practice is increasingly widespread. Most of the existing evidence concerns myoinositol (MYO), the most abundant form in nature, especially in women with polycystic ovarian syndrome. We have previously shown that MYO increases sperm motility in patie...
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doaj-34d1729a8aa84c57a4d6b007af03fe672020-11-25T02:58:12ZengMDPI AGJournal of Clinical Medicine2077-03832020-05-0191373137310.3390/jcm9051373D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro EvidenceRosita A. Condorelli0Federica Barbagallo1Aldo E. Calogero2Rossella Cannarella3Andrea Crafa4Sandro La Vignera5Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95123 Catania, ItalyThe use of inositols in endocrinological clinical practice is increasingly widespread. Most of the existing evidence concerns myoinositol (MYO), the most abundant form in nature, especially in women with polycystic ovarian syndrome. We have previously shown that MYO increases sperm motility in patients with asthenozoospermia by the increase of sperm mitochondrial membrane potential (MMP), a biofunctional sperm parameter closely associated to sperm motility. The aim of this study was to evaluate the effects of D-chiro-inositol (DCI), another biologically active isoform of inositols, on sperm MMP, as data on this matter has never been released so far. To accomplish this, semen samples from 15 patients with asthenozoospermia and 15 healthy normozoospermic men were incubated with increasing concentrations of DCI (0, 75, and 750 µg/mL) or phosphate buffer saline for 30 min. Incubation with DCI significantly improved sperm MMP at lower concentrations, and with shorter incubation length than those used in our similar MYO studies. In conclusion, these findings indicate that DCI positively impacts on sperm mitochondrial function in vitro. Studies aimed at assessing the role of DCI in the treatment of asthenozoospermia in-vivo are warranted.https://www.mdpi.com/2077-0383/9/5/1373D-chiro-inositolmale infertilitysperm mitochondrial membrane potential |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosita A. Condorelli Federica Barbagallo Aldo E. Calogero Rossella Cannarella Andrea Crafa Sandro La Vignera |
spellingShingle |
Rosita A. Condorelli Federica Barbagallo Aldo E. Calogero Rossella Cannarella Andrea Crafa Sandro La Vignera D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence Journal of Clinical Medicine D-chiro-inositol male infertility sperm mitochondrial membrane potential |
author_facet |
Rosita A. Condorelli Federica Barbagallo Aldo E. Calogero Rossella Cannarella Andrea Crafa Sandro La Vignera |
author_sort |
Rosita A. Condorelli |
title |
D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence |
title_short |
D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence |
title_full |
D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence |
title_fullStr |
D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence |
title_full_unstemmed |
D-Chiro-Inositol Improves Sperm Mitochondrial Membrane Potential: In Vitro Evidence |
title_sort |
d-chiro-inositol improves sperm mitochondrial membrane potential: in vitro evidence |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-05-01 |
description |
The use of inositols in endocrinological clinical practice is increasingly widespread. Most of the existing evidence concerns myoinositol (MYO), the most abundant form in nature, especially in women with polycystic ovarian syndrome. We have previously shown that MYO increases sperm motility in patients with asthenozoospermia by the increase of sperm mitochondrial membrane potential (MMP), a biofunctional sperm parameter closely associated to sperm motility. The aim of this study was to evaluate the effects of D-chiro-inositol (DCI), another biologically active isoform of inositols, on sperm MMP, as data on this matter has never been released so far. To accomplish this, semen samples from 15 patients with asthenozoospermia and 15 healthy normozoospermic men were incubated with increasing concentrations of DCI (0, 75, and 750 µg/mL) or phosphate buffer saline for 30 min. Incubation with DCI significantly improved sperm MMP at lower concentrations, and with shorter incubation length than those used in our similar MYO studies. In conclusion, these findings indicate that DCI positively impacts on sperm mitochondrial function in vitro. Studies aimed at assessing the role of DCI in the treatment of asthenozoospermia in-vivo are warranted. |
topic |
D-chiro-inositol male infertility sperm mitochondrial membrane potential |
url |
https://www.mdpi.com/2077-0383/9/5/1373 |
work_keys_str_mv |
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