Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway
Resveratrol, a polyphenol found in various plants, including grapes, plums and peanuts has shown various medIRInal properties, including antioxidant, protection of cardiovascular disease and cancer risk. However, the effects of resveratrol on spinal cord reperfusion injury have not been investigated...
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doaj-34e53331bb6045f490be11a4cff0a85e2020-11-24T23:15:39ZengElsevierSaudi Journal of Biological Sciences1319-562X2018-02-0125225926610.1016/j.sjbs.2016.10.019Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathwaySong Fu0Renhua Lv1Longqiang Wang2Haitao Hou3Haijun Liu4Shize Shao5Department of Spinal, The Wendeng Osteopathic Hospital, Weihai City, Shandong Province 264400, ChinaDepartment of Orthopaedics, Wendeng Central Hospital, Weihai City, Shandong Province 264400, ChinaDepartment of Spinal, The Wendeng Osteopathic Hospital, Weihai City, Shandong Province 264400, ChinaDepartment of Spinal, The Wendeng Osteopathic Hospital, Weihai City, Shandong Province 264400, ChinaDepartment of Spinal, The Wendeng Osteopathic Hospital, Weihai City, Shandong Province 264400, ChinaDepartment of Spinal, The Wendeng Osteopathic Hospital, Weihai City, Shandong Province 264400, ChinaResveratrol, a polyphenol found in various plants, including grapes, plums and peanuts has shown various medIRInal properties, including antioxidant, protection of cardiovascular disease and cancer risk. However, the effects of resveratrol on spinal cord reperfusion injury have not been investigated. Hence, the present study was designed to evaluate the effect of resveratrol on nitric oxide synthase (iNOS)/p38MAPK signaling pathway and to elucidate its regulating effect on the protection of spinal cord injury. Spinal cord ischemia–reperfusion injury (IRI) was performed by the infrarenal abdominal aorta with mini aneurysm clip model. The expressions of iNOS and p38MAPK and the levels of biochemical parameters, including nitrite/nitrate, malondialdehyde (MDA), advanced oxidation products (AOPP), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were measured in control and experimental groups. IRI-induced rats treated with 10 mg/kg resveratrol protected spinal cord from ischemia injury as supported by improved biological parameters measured in spinal cord tissue homogenates. The resveratrol treatment significantly decreased the levels of plasma nitrite/nitrate, iNOS mRNA and protein expressions and phosphorylation of p38MAPK in IRI-induced rats. Further, IRI-produced free radicals were reduced by resveratrol treatment by increasing enzymatic and non-enzymatic antioxidant levels such as GSH, SOD and CAT. Taken together, administration of resveratrol protects the damage caused by spinal cord ischemia with potential mechanism of suppressing the activation of iNOS/p38MAPK pathway and subsequent reduction of oxidative stress due to IRI.http://www.sciencedirect.com/science/article/pii/S1319562X16301437Spinal cordResveratrolAntioxidantp38MAPkiNOS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Song Fu Renhua Lv Longqiang Wang Haitao Hou Haijun Liu Shize Shao |
spellingShingle |
Song Fu Renhua Lv Longqiang Wang Haitao Hou Haijun Liu Shize Shao Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway Saudi Journal of Biological Sciences Spinal cord Resveratrol Antioxidant p38MAPk iNOS |
author_facet |
Song Fu Renhua Lv Longqiang Wang Haitao Hou Haijun Liu Shize Shao |
author_sort |
Song Fu |
title |
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway |
title_short |
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway |
title_full |
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway |
title_fullStr |
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway |
title_full_unstemmed |
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway |
title_sort |
resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing mapk pathway |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2018-02-01 |
description |
Resveratrol, a polyphenol found in various plants, including grapes, plums and peanuts has shown various medIRInal properties, including antioxidant, protection of cardiovascular disease and cancer risk. However, the effects of resveratrol on spinal cord reperfusion injury have not been investigated. Hence, the present study was designed to evaluate the effect of resveratrol on nitric oxide synthase (iNOS)/p38MAPK signaling pathway and to elucidate its regulating effect on the protection of spinal cord injury. Spinal cord ischemia–reperfusion injury (IRI) was performed by the infrarenal abdominal aorta with mini aneurysm clip model. The expressions of iNOS and p38MAPK and the levels of biochemical parameters, including nitrite/nitrate, malondialdehyde (MDA), advanced oxidation products (AOPP), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were measured in control and experimental groups. IRI-induced rats treated with 10 mg/kg resveratrol protected spinal cord from ischemia injury as supported by improved biological parameters measured in spinal cord tissue homogenates. The resveratrol treatment significantly decreased the levels of plasma nitrite/nitrate, iNOS mRNA and protein expressions and phosphorylation of p38MAPK in IRI-induced rats. Further, IRI-produced free radicals were reduced by resveratrol treatment by increasing enzymatic and non-enzymatic antioxidant levels such as GSH, SOD and CAT. Taken together, administration of resveratrol protects the damage caused by spinal cord ischemia with potential mechanism of suppressing the activation of iNOS/p38MAPK pathway and subsequent reduction of oxidative stress due to IRI. |
topic |
Spinal cord Resveratrol Antioxidant p38MAPk iNOS |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X16301437 |
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