Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-...

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Main Authors: Bootsakorn Boonkaew, Weeradee Thummavichit, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch
Format: Article
Language:English
Published: Elsevier 2018-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350611830120X
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spelling doaj-34e6f6c2e9ac4ba79b75bd56b1494b002020-11-24T21:32:59ZengElsevierStem Cell Research1873-50612018-07-01303437Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cellsBootsakorn Boonkaew0Weeradee Thummavichit1Ratchapong Netsrithong2Chinnavuth Vatanashevanopakorn3Kovit Pattanapanyasat4Methichit Wattanapanitch5Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biology, Faculty of Science, Silpakorn University, Nakornpathom, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center of Research Excellence for Microparticle and Exosome in Diseases, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding author.Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.http://www.sciencedirect.com/science/article/pii/S187350611830120X
collection DOAJ
language English
format Article
sources DOAJ
author Bootsakorn Boonkaew
Weeradee Thummavichit
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
spellingShingle Bootsakorn Boonkaew
Weeradee Thummavichit
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
Stem Cell Research
author_facet Bootsakorn Boonkaew
Weeradee Thummavichit
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
author_sort Bootsakorn Boonkaew
title Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
title_short Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
title_full Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
title_fullStr Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
title_full_unstemmed Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
title_sort establishment of an integration-free induced pluripotent stem cell line (musii005-a) from exfoliated renal epithelial cells
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-07-01
description Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.
url http://www.sciencedirect.com/science/article/pii/S187350611830120X
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AT weeradeethummavichit establishmentofanintegrationfreeinducedpluripotentstemcelllinemusii005afromexfoliatedrenalepithelialcells
AT ratchapongnetsrithong establishmentofanintegrationfreeinducedpluripotentstemcelllinemusii005afromexfoliatedrenalepithelialcells
AT chinnavuthvatanashevanopakorn establishmentofanintegrationfreeinducedpluripotentstemcelllinemusii005afromexfoliatedrenalepithelialcells
AT kovitpattanapanyasat establishmentofanintegrationfreeinducedpluripotentstemcelllinemusii005afromexfoliatedrenalepithelialcells
AT methichitwattanapanitch establishmentofanintegrationfreeinducedpluripotentstemcelllinemusii005afromexfoliatedrenalepithelialcells
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