Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters
This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the int...
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doaj-34ffa2cb51ec43d0a01b4913a2d1734f2020-11-24T23:54:50ZengMDPI AGSensors1424-82202019-05-011911249310.3390/s19112493s19112493Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth ParametersMarco César Prado Soares0Franciele Flores Vit1Carlos Kenichi Suzuki2Lucimara Gaziola de la Torre3Eric Fujiwara4Laboratory of Photonic Materials and Devices, School of Mechanical Engineering, University of Campinas, São Paulo 13083-860, BrazilLaboratory of Advanced Development of Nano and Biotechnology, School of Chemical Engineering, University of Campinas, São Paulo 13083-852, BrazilLaboratory of Photonic Materials and Devices, School of Mechanical Engineering, University of Campinas, São Paulo 13083-860, BrazilLaboratory of Advanced Development of Nano and Biotechnology, School of Chemical Engineering, University of Campinas, São Paulo 13083-852, BrazilLaboratory of Photonic Materials and Devices, School of Mechanical Engineering, University of Campinas, São Paulo 13083-860, BrazilThis research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the interior of perfusion chambers, which are separated by polycarbonate membranes (470 nm pores) from microchannels, where a culture medium flows in a constant concentration. The system contains four layers, a superior and an inferior layer made of glass, and two intermediate poly(dimethylsiloxane) layers that contain the microchannels and the perfusion chambers, forming a reversible microfluidic device that requires only the sealing of the fibers to the inferior glass cover. The QELS autocorrelation decay rates of the optical signals were correlated to the cells counting in a microscope, and the application of this microsystem to the monitoring of alcoholic fermentation of <i>Saccharomyces cerevisiae</i> resulted in the kinetic parameters of K<sub>M</sub> = 4.1 g/L and μ<sub>m</sub> = 0.49 h<sup>−1</sup>. These results agree with both the data reported in the literature and with the control batch test, showing that it is a reliable and efficient biological monitoring system.https://www.mdpi.com/1424-8220/19/11/2493fiber optic sensormicrofermentorquasi-elastic light scatteringmicrobial growth screeningbiological monitoring |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco César Prado Soares Franciele Flores Vit Carlos Kenichi Suzuki Lucimara Gaziola de la Torre Eric Fujiwara |
spellingShingle |
Marco César Prado Soares Franciele Flores Vit Carlos Kenichi Suzuki Lucimara Gaziola de la Torre Eric Fujiwara Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters Sensors fiber optic sensor microfermentor quasi-elastic light scattering microbial growth screening biological monitoring |
author_facet |
Marco César Prado Soares Franciele Flores Vit Carlos Kenichi Suzuki Lucimara Gaziola de la Torre Eric Fujiwara |
author_sort |
Marco César Prado Soares |
title |
Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_short |
Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_full |
Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_fullStr |
Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_full_unstemmed |
Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_sort |
perfusion microfermentor integrated into a fiber optic quasi-elastic light scattering sensor for fast screening of microbial growth parameters |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2019-05-01 |
description |
This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the interior of perfusion chambers, which are separated by polycarbonate membranes (470 nm pores) from microchannels, where a culture medium flows in a constant concentration. The system contains four layers, a superior and an inferior layer made of glass, and two intermediate poly(dimethylsiloxane) layers that contain the microchannels and the perfusion chambers, forming a reversible microfluidic device that requires only the sealing of the fibers to the inferior glass cover. The QELS autocorrelation decay rates of the optical signals were correlated to the cells counting in a microscope, and the application of this microsystem to the monitoring of alcoholic fermentation of <i>Saccharomyces cerevisiae</i> resulted in the kinetic parameters of K<sub>M</sub> = 4.1 g/L and μ<sub>m</sub> = 0.49 h<sup>−1</sup>. These results agree with both the data reported in the literature and with the control batch test, showing that it is a reliable and efficient biological monitoring system. |
topic |
fiber optic sensor microfermentor quasi-elastic light scattering microbial growth screening biological monitoring |
url |
https://www.mdpi.com/1424-8220/19/11/2493 |
work_keys_str_mv |
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