Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon
Abstract Background African trypanosomes are mainly transmitted through the bite of tsetse flies (Glossina spp.). The present study investigated the occurrence of pathogenic trypanosomes in tsetse flies and cattle in tsetse fly-infested areas of Northern Cameroon. Results Trypanosomes were identifie...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-12-01
|
Series: | Parasites & Vectors |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13071-017-2540-7 |
id |
doaj-3509d99bd1e94ea38d808de92fc22a04 |
---|---|
record_format |
Article |
spelling |
doaj-3509d99bd1e94ea38d808de92fc22a042020-11-25T00:47:48ZengBMCParasites & Vectors1756-33052017-12-0110111610.1186/s13071-017-2540-7Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern CameroonSen Claudine Henriette Ngomtcho0Judith Sophie Weber1Elisabeth Ngo Bum2Thaddeus Terlumun Gbem3Sørge Kelm4Mbunkah Daniel Achukwi5Department of Biological Sciences, University NgaoundéréCentre for Biomolecular Interactions Bremen, Department of Biology and Chemistry, University BremenDepartment of Biological Sciences, University NgaoundéréAfrica Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello UniversityCentre for Biomolecular Interactions Bremen, Department of Biology and Chemistry, University BremenTOZARD Research LaboratoryAbstract Background African trypanosomes are mainly transmitted through the bite of tsetse flies (Glossina spp.). The present study investigated the occurrence of pathogenic trypanosomes in tsetse flies and cattle in tsetse fly-infested areas of Northern Cameroon. Results Trypanosomes were identified using nested polymerase chain reaction (PCR) analysis of internal transcribed spacer 1 (ITS1) region, both by size estimation and sequencing of PCR products. Apparent density indices recorded in Gamba and Dodeo were 3.1 and 3.6 tsetse flies per trap and day, respectively. Trypanosoma prevalence infection rate for the tsetse fly gut (40%) and proboscis (19%) were recorded. Among the flies where trypanosomes were detected in the gut, 41.7% were positive for T. congolense and 14.6% for T. brucei ssp., whereas in the proboscis 36% harboured T. congolense and 62% contained T. vivax. T. grayi was highly prevalent in tsetse fly gut (58%). The most common mixed infections were the combination of T. congolense and T. grayi. Trypanosome prevalence rate in cattle blood was 6%. Among these, T. vivax represented 26%, T. congolense 35%, T. brucei ssp. 17% and T. theileri 17% of the infections. Surprisingly, in one case T. grayi was found in cattle. The mean packed cell volume (PCV) of cattle positive for trypanosomes was significantly lower (24.1 ± 5.6%; P < 0.05) than that of cattle in which trypanosomes were not detected (27.1 ± 4.9%). Interestingly, the occurrence of T. theileri or T. grayi DNA in cattle also correlated with low PCV at pathological levels. Conclusion This molecular epidemiological study of Trypanosoma species in Northern Cameroon revealed active foci of trypanosomes in Dodeo and Gamba. These findings are relevant in assessing the status of trypanosomosis in these regions and will serve as a guide for setting the priorities of the government in the control of the disease.http://link.springer.com/article/10.1186/s13071-017-2540-7Trypanosoma grayiTrypanosoma theileriBodonidaeCattleTsetse flyITS1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sen Claudine Henriette Ngomtcho Judith Sophie Weber Elisabeth Ngo Bum Thaddeus Terlumun Gbem Sørge Kelm Mbunkah Daniel Achukwi |
spellingShingle |
Sen Claudine Henriette Ngomtcho Judith Sophie Weber Elisabeth Ngo Bum Thaddeus Terlumun Gbem Sørge Kelm Mbunkah Daniel Achukwi Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon Parasites & Vectors Trypanosoma grayi Trypanosoma theileri Bodonidae Cattle Tsetse fly ITS1 |
author_facet |
Sen Claudine Henriette Ngomtcho Judith Sophie Weber Elisabeth Ngo Bum Thaddeus Terlumun Gbem Sørge Kelm Mbunkah Daniel Achukwi |
author_sort |
Sen Claudine Henriette Ngomtcho |
title |
Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon |
title_short |
Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon |
title_full |
Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon |
title_fullStr |
Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon |
title_full_unstemmed |
Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon |
title_sort |
molecular screening of tsetse flies and cattle reveal different trypanosoma species including t. grayi and t. theileri in northern cameroon |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2017-12-01 |
description |
Abstract Background African trypanosomes are mainly transmitted through the bite of tsetse flies (Glossina spp.). The present study investigated the occurrence of pathogenic trypanosomes in tsetse flies and cattle in tsetse fly-infested areas of Northern Cameroon. Results Trypanosomes were identified using nested polymerase chain reaction (PCR) analysis of internal transcribed spacer 1 (ITS1) region, both by size estimation and sequencing of PCR products. Apparent density indices recorded in Gamba and Dodeo were 3.1 and 3.6 tsetse flies per trap and day, respectively. Trypanosoma prevalence infection rate for the tsetse fly gut (40%) and proboscis (19%) were recorded. Among the flies where trypanosomes were detected in the gut, 41.7% were positive for T. congolense and 14.6% for T. brucei ssp., whereas in the proboscis 36% harboured T. congolense and 62% contained T. vivax. T. grayi was highly prevalent in tsetse fly gut (58%). The most common mixed infections were the combination of T. congolense and T. grayi. Trypanosome prevalence rate in cattle blood was 6%. Among these, T. vivax represented 26%, T. congolense 35%, T. brucei ssp. 17% and T. theileri 17% of the infections. Surprisingly, in one case T. grayi was found in cattle. The mean packed cell volume (PCV) of cattle positive for trypanosomes was significantly lower (24.1 ± 5.6%; P < 0.05) than that of cattle in which trypanosomes were not detected (27.1 ± 4.9%). Interestingly, the occurrence of T. theileri or T. grayi DNA in cattle also correlated with low PCV at pathological levels. Conclusion This molecular epidemiological study of Trypanosoma species in Northern Cameroon revealed active foci of trypanosomes in Dodeo and Gamba. These findings are relevant in assessing the status of trypanosomosis in these regions and will serve as a guide for setting the priorities of the government in the control of the disease. |
topic |
Trypanosoma grayi Trypanosoma theileri Bodonidae Cattle Tsetse fly ITS1 |
url |
http://link.springer.com/article/10.1186/s13071-017-2540-7 |
work_keys_str_mv |
AT senclaudinehenriettengomtcho molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon AT judithsophieweber molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon AT elisabethngobum molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon AT thaddeusterlumungbem molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon AT sørgekelm molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon AT mbunkahdanielachukwi molecularscreeningoftsetsefliesandcattlerevealdifferenttrypanosomaspeciesincludingtgrayiandttheileriinnortherncameroon |
_version_ |
1725258468115349504 |