Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon
Abstract Background Shikonin is a naphthoquinone secondary metabolite with important medicinal value and is found in Lithospermum erythrorhizon. Considering the limited knowledge on the membrane transport mechanism of shikonin, this study investigated such molecular mechanism. Results We successfull...
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doaj-350cfe59397a4d1ca1a08c212f7c38cb2020-11-25T01:41:59ZengBMCBMC Plant Biology1471-22292017-11-0117111010.1186/s12870-017-1148-6Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizonYu Zhu0Gui-Hua Lu1Zhuo-Wu Bian2Feng-Yao Wu3Yan-Jun Pang4Xiao-Ming Wang5Rong-Wu Yang6Cheng-Yi Tang7Jin-Liang Qi8Yong-Hua Yang9Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing UniversityAbstract Background Shikonin is a naphthoquinone secondary metabolite with important medicinal value and is found in Lithospermum erythrorhizon. Considering the limited knowledge on the membrane transport mechanism of shikonin, this study investigated such molecular mechanism. Results We successfully isolated an ATP-binding cassette protein gene, LeMDR, from L. erythrorhizon. LeMDR is predominantly expressed in L. erythrorhizon roots, where shikonin accumulated. Functional analysis of LeMDR by using the yeast cell expression system revealed that LeMDR is possibly involved in the shikonin efflux transport. The accumulation of shikonin is lower in yeast cells transformed with LeMDR-overexpressing vector than that with empty vector. The transgenic hairy roots of L. erythrorhizon overexpressing LeMDR (MDRO) significantly enhanced shikonin production, whereas the RNA interference of LeMDR (MDRi) displayed a reverse trend. Moreover, the mRNA expression level of LeMDR was up-regulated by treatment with shikonin and shikonin-positive regulators, methyl jasmonate and indole-3-acetic acid. There might be a relationship of mutual regulation between the expression level of LeMDR and shikonin biosynthesis. Conclusions Our findings demonstrated the important role of LeMDR in transmembrane transport and biosynthesis of shikonin.http://link.springer.com/article/10.1186/s12870-017-1148-6ABC transporterLeMDRHairy rootOverexpressionRNAiShikonin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Zhu Gui-Hua Lu Zhuo-Wu Bian Feng-Yao Wu Yan-Jun Pang Xiao-Ming Wang Rong-Wu Yang Cheng-Yi Tang Jin-Liang Qi Yong-Hua Yang |
spellingShingle |
Yu Zhu Gui-Hua Lu Zhuo-Wu Bian Feng-Yao Wu Yan-Jun Pang Xiao-Ming Wang Rong-Wu Yang Cheng-Yi Tang Jin-Liang Qi Yong-Hua Yang Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon BMC Plant Biology ABC transporter LeMDR Hairy root Overexpression RNAi Shikonin |
author_facet |
Yu Zhu Gui-Hua Lu Zhuo-Wu Bian Feng-Yao Wu Yan-Jun Pang Xiao-Ming Wang Rong-Wu Yang Cheng-Yi Tang Jin-Liang Qi Yong-Hua Yang |
author_sort |
Yu Zhu |
title |
Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon |
title_short |
Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon |
title_full |
Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon |
title_fullStr |
Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon |
title_full_unstemmed |
Involvement of LeMDR, an ATP-binding cassette protein gene, in shikonin transport and biosynthesis in Lithospermum erythrorhizon |
title_sort |
involvement of lemdr, an atp-binding cassette protein gene, in shikonin transport and biosynthesis in lithospermum erythrorhizon |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2017-11-01 |
description |
Abstract Background Shikonin is a naphthoquinone secondary metabolite with important medicinal value and is found in Lithospermum erythrorhizon. Considering the limited knowledge on the membrane transport mechanism of shikonin, this study investigated such molecular mechanism. Results We successfully isolated an ATP-binding cassette protein gene, LeMDR, from L. erythrorhizon. LeMDR is predominantly expressed in L. erythrorhizon roots, where shikonin accumulated. Functional analysis of LeMDR by using the yeast cell expression system revealed that LeMDR is possibly involved in the shikonin efflux transport. The accumulation of shikonin is lower in yeast cells transformed with LeMDR-overexpressing vector than that with empty vector. The transgenic hairy roots of L. erythrorhizon overexpressing LeMDR (MDRO) significantly enhanced shikonin production, whereas the RNA interference of LeMDR (MDRi) displayed a reverse trend. Moreover, the mRNA expression level of LeMDR was up-regulated by treatment with shikonin and shikonin-positive regulators, methyl jasmonate and indole-3-acetic acid. There might be a relationship of mutual regulation between the expression level of LeMDR and shikonin biosynthesis. Conclusions Our findings demonstrated the important role of LeMDR in transmembrane transport and biosynthesis of shikonin. |
topic |
ABC transporter LeMDR Hairy root Overexpression RNAi Shikonin |
url |
http://link.springer.com/article/10.1186/s12870-017-1148-6 |
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