Study of vasodilation processes
<p>To date, the key role of endothelial dysfunction in the occurrence and progression of diabetes mellitus and diabetic retinopathy has been proven. The study was performed on white Wistar rats weighing 180-200 g. According to the tasks, the animals were divided into 7 groups. Our results indi...
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Kazimierz Wielki University
2019-12-01
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doaj-3512c377bf244a6d99c913aef84562db2021-09-08T14:44:32ZengKazimierz Wielki UniversityJournal of Education, Health and Sport2391-83062019-12-0191232533710.12775/JEHS.2019.09.12.03326607Study of vasodilation processesYa. V. Sirman0I. V. Savytskyi1Ukrainian Research Institute of Transport Medicine of the Ministry of Health of UkraineOdessa International Medical University<p>To date, the key role of endothelial dysfunction in the occurrence and progression of diabetes mellitus and diabetic retinopathy has been proven. The study was performed on white Wistar rats weighing 180-200 g. According to the tasks, the animals were divided into 7 groups. Our results indicate a violation of vasodilation on the 30th day of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180<sup>th</sup> day of the study, as evidenced by a decrease in the content of S-nitrosothiols in group 2 (p <0,001 ), most pronounced in the 3<sup>rd</sup> stage. When analyzing the data of group № 3, it was found that the correction of the pathological condition with the help of hypoglycemic agents has some positive effect, but does not allow to significantly adjust the pathological development of reduced vasodilatory potential. The results of the 4<sup>th</sup> group indicate that the involvement of nitric oxide donor and aflibercept in the correction of diabetic retinopathy corrects the pathological changes and helps to restore the physiological pathway of nitric oxide synthesis and vascular tone, the maximum effect is observed on the 180<sup>th</sup> day of the experiment, but normative cannot be achieved. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group № 5) gives positive results in the first stage, but less pronounced than involvement in the complex correction of L-arginine solution in subsequent stages. It was found that rats in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group № 6) have a pronounced tendency to normalize the studied marker of hypoxia in comparison with the previous methods in the second stage. nitrosothiols at the time of reaching the 3<sup>rd</sup> stage is reduced. The obtained data suggest that the method of correction chosen in group 7 (correction of hyperglycemia, aflibercept, L-arginine and citicoline solution) more pronouncedly normalizes the content of the vasodilation marker compared to other groups of our experiment, which is pronounced in long-term correction - on 180<sup>th</sup> day.</p>https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/33084experimental diabetic retinopathyendothelial dysfunctionvasodilations-nitrosothiolscorrectionmetforminafliberceptl-arginineciticolinel-carnitinebromfenac |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya. V. Sirman I. V. Savytskyi |
spellingShingle |
Ya. V. Sirman I. V. Savytskyi Study of vasodilation processes Journal of Education, Health and Sport experimental diabetic retinopathy endothelial dysfunction vasodilation s-nitrosothiols correction metformin aflibercept l-arginine citicoline l-carnitine bromfenac |
author_facet |
Ya. V. Sirman I. V. Savytskyi |
author_sort |
Ya. V. Sirman |
title |
Study of vasodilation processes |
title_short |
Study of vasodilation processes |
title_full |
Study of vasodilation processes |
title_fullStr |
Study of vasodilation processes |
title_full_unstemmed |
Study of vasodilation processes |
title_sort |
study of vasodilation processes |
publisher |
Kazimierz Wielki University |
series |
Journal of Education, Health and Sport |
issn |
2391-8306 |
publishDate |
2019-12-01 |
description |
<p>To date, the key role of endothelial dysfunction in the occurrence and progression of diabetes mellitus and diabetic retinopathy has been proven. The study was performed on white Wistar rats weighing 180-200 g. According to the tasks, the animals were divided into 7 groups. Our results indicate a violation of vasodilation on the 30th day of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180<sup>th</sup> day of the study, as evidenced by a decrease in the content of S-nitrosothiols in group 2 (p <0,001 ), most pronounced in the 3<sup>rd</sup> stage. When analyzing the data of group № 3, it was found that the correction of the pathological condition with the help of hypoglycemic agents has some positive effect, but does not allow to significantly adjust the pathological development of reduced vasodilatory potential. The results of the 4<sup>th</sup> group indicate that the involvement of nitric oxide donor and aflibercept in the correction of diabetic retinopathy corrects the pathological changes and helps to restore the physiological pathway of nitric oxide synthesis and vascular tone, the maximum effect is observed on the 180<sup>th</sup> day of the experiment, but normative cannot be achieved. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group № 5) gives positive results in the first stage, but less pronounced than involvement in the complex correction of L-arginine solution in subsequent stages. It was found that rats in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group № 6) have a pronounced tendency to normalize the studied marker of hypoxia in comparison with the previous methods in the second stage. nitrosothiols at the time of reaching the 3<sup>rd</sup> stage is reduced. The obtained data suggest that the method of correction chosen in group 7 (correction of hyperglycemia, aflibercept, L-arginine and citicoline solution) more pronouncedly normalizes the content of the vasodilation marker compared to other groups of our experiment, which is pronounced in long-term correction - on 180<sup>th</sup> day.</p> |
topic |
experimental diabetic retinopathy endothelial dysfunction vasodilation s-nitrosothiols correction metformin aflibercept l-arginine citicoline l-carnitine bromfenac |
url |
https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/33084 |
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