Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths
To identify mechanisms of starch degradation in rice leaf sheaths at the post-heading stage, we investigated the function of OsBAM2 and OsBAM3, which encode plastid-targeted active β-amylase isoforms, in starch remobilization in leaf sheaths. The starch content in the second leaf sheaths below the f...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2016-04-01
|
Series: | Plant Production Science |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/1343943X.2016.1140008 |
id |
doaj-351956fd0a08407094e59b8e277e9244 |
---|---|
record_format |
Article |
spelling |
doaj-351956fd0a08407094e59b8e277e92442020-11-24T23:47:37ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082016-04-0119229129910.1080/1343943X.2016.11400081140008Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheathsTatsuya Hirano0Takayuki Higuchi1Minako Hirano2Yu Sugimura3Hiroyasu Michiyama4Meijo UniversityMeijo UniversityMeijo UniversityMeijo UniversityMeijo UniversityTo identify mechanisms of starch degradation in rice leaf sheaths at the post-heading stage, we investigated the function of OsBAM2 and OsBAM3, which encode plastid-targeted active β-amylase isoforms, in starch remobilization in leaf sheaths. The starch content in the second leaf sheaths below the flag leaf (the third leaf sheaths) peaked at the flag leaf emergence stage and gradually decreased until 15 days after heading. The mRNA levels of OsBAM2 and OsBAM3 in the third leaf sheaths increased from the flag leaf emergence stage to the heading stage when the starch content began to decrease. However, these mRNA levels did not always remain high during post-heading. Overexpression of OsBAM2 or OsBAM3 markedly repressed starch accumulation in the third leaf sheaths, showing that OsBAM2 and OsBAM3 function in starch degradation in rice leaf sheaths. In contrast, no significant differences in starch content in the third leaf sheaths were detected between knockdown plants of OsBAM2 or OsBAM3 and non-transgenic wild-type plants. Our results suggest that reduced expression of the individual genes, OsBAM2 or OsBAM3, does not result in excess accumulation of starch in the leaf sheaths, probably because of the complementary function of another gene or the action of other genes encoding starch-degrading .http://dx.doi.org/10.1080/1343943X.2016.1140008β-Amylaseleaf sheathOryza sativa Lstarch degradation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tatsuya Hirano Takayuki Higuchi Minako Hirano Yu Sugimura Hiroyasu Michiyama |
spellingShingle |
Tatsuya Hirano Takayuki Higuchi Minako Hirano Yu Sugimura Hiroyasu Michiyama Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths Plant Production Science β-Amylase leaf sheath Oryza sativa L starch degradation |
author_facet |
Tatsuya Hirano Takayuki Higuchi Minako Hirano Yu Sugimura Hiroyasu Michiyama |
author_sort |
Tatsuya Hirano |
title |
Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths |
title_short |
Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths |
title_full |
Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths |
title_fullStr |
Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths |
title_full_unstemmed |
Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths |
title_sort |
two β-amylase genes, osbam2 and osbam3, are involved in starch remobilization in rice leaf sheaths |
publisher |
Taylor & Francis Group |
series |
Plant Production Science |
issn |
1343-943X 1349-1008 |
publishDate |
2016-04-01 |
description |
To identify mechanisms of starch degradation in rice leaf sheaths at the post-heading stage, we investigated the function of OsBAM2 and OsBAM3, which encode plastid-targeted active β-amylase isoforms, in starch remobilization in leaf sheaths. The starch content in the second leaf sheaths below the flag leaf (the third leaf sheaths) peaked at the flag leaf emergence stage and gradually decreased until 15 days after heading. The mRNA levels of OsBAM2 and OsBAM3 in the third leaf sheaths increased from the flag leaf emergence stage to the heading stage when the starch content began to decrease. However, these mRNA levels did not always remain high during post-heading. Overexpression of OsBAM2 or OsBAM3 markedly repressed starch accumulation in the third leaf sheaths, showing that OsBAM2 and OsBAM3 function in starch degradation in rice leaf sheaths. In contrast, no significant differences in starch content in the third leaf sheaths were detected between knockdown plants of OsBAM2 or OsBAM3 and non-transgenic wild-type plants. Our results suggest that reduced expression of the individual genes, OsBAM2 or OsBAM3, does not result in excess accumulation of starch in the leaf sheaths, probably because of the complementary function of another gene or the action of other genes encoding starch-degrading . |
topic |
β-Amylase leaf sheath Oryza sativa L starch degradation |
url |
http://dx.doi.org/10.1080/1343943X.2016.1140008 |
work_keys_str_mv |
AT tatsuyahirano twobamylasegenesosbam2andosbam3areinvolvedinstarchremobilizationinriceleafsheaths AT takayukihiguchi twobamylasegenesosbam2andosbam3areinvolvedinstarchremobilizationinriceleafsheaths AT minakohirano twobamylasegenesosbam2andosbam3areinvolvedinstarchremobilizationinriceleafsheaths AT yusugimura twobamylasegenesosbam2andosbam3areinvolvedinstarchremobilizationinriceleafsheaths AT hiroyasumichiyama twobamylasegenesosbam2andosbam3areinvolvedinstarchremobilizationinriceleafsheaths |
_version_ |
1725488895131385856 |