A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria
<div>Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from Southeast</div><div>Sulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study was</div><div>attempted to determine...
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Universitas Gadjah Mada, Yogyakarta
2015-11-01
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Series: | Indonesian Journal of Biotechnology |
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doaj-35220281b6be41ec90a3e4802f6fb5f72020-11-24T23:21:44ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011826593A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic BacteriaNur Arfa YantiLangkah SembiringSebastian MarginoNurhayani H. Muhiddin<div>Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from Southeast</div><div>Sulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study was</div><div>attempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontaining</div><div>media. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 h</div><div>in a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitored</div><div>by cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB content</div><div>was quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucose</div><div>GOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determined</div><div>by phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.</div><div>PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83</div><div>% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directly</div><div>without glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as product</div><div>of sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starch</div><div>of the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be non</div><div>growth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesis</div><div>mechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be considered</div><div>in developing cultivation methods for the effi cient production of PHB.</div><div><br /></div><div>Keywords : Production, PHB, Amylolytic bacteria, Sago starch.</div>http://journal.ugm.ac.id/ijbiotech/article/view/7877 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nur Arfa Yanti Langkah Sembiring Sebastian Margino Nurhayani H. Muhiddin |
spellingShingle |
Nur Arfa Yanti Langkah Sembiring Sebastian Margino Nurhayani H. Muhiddin A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Indonesian Journal of Biotechnology |
author_facet |
Nur Arfa Yanti Langkah Sembiring Sebastian Margino Nurhayani H. Muhiddin |
author_sort |
Nur Arfa Yanti |
title |
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria |
title_short |
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria |
title_full |
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria |
title_fullStr |
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria |
title_full_unstemmed |
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria |
title_sort |
study on production of poly-β-hydroxybutyrate bioplastic from sago starch by indigenous amylolytic bacteria |
publisher |
Universitas Gadjah Mada, Yogyakarta |
series |
Indonesian Journal of Biotechnology |
issn |
0853-8654 2089-2241 |
publishDate |
2015-11-01 |
description |
<div>Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from Southeast</div><div>Sulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study was</div><div>attempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontaining</div><div>media. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 h</div><div>in a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitored</div><div>by cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB content</div><div>was quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucose</div><div>GOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determined</div><div>by phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.</div><div>PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83</div><div>% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directly</div><div>without glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as product</div><div>of sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starch</div><div>of the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be non</div><div>growth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesis</div><div>mechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be considered</div><div>in developing cultivation methods for the effi cient production of PHB.</div><div><br /></div><div>Keywords : Production, PHB, Amylolytic bacteria, Sago starch.</div> |
url |
http://journal.ugm.ac.id/ijbiotech/article/view/7877 |
work_keys_str_mv |
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