Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins
The use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D402...
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doaj-352664a2b26744a48f7472f54755c5fe2020-11-24T23:01:54ZengMDPI AGMolecules1420-30492016-06-0121676810.3390/molecules21060768molecules21060768Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous ResinsYang Xie0Qiu-Shi Guo1Guang-Shu Wang2Department of Pharmacognosy, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaDepartment of Pharmacy, the Second Part of the First Hospital, Jilin University, Changchun 130021, ChinaDepartment of Pharmacognosy, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaThe use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D4020 resin columns were loaded with crude flavonoid extract solution, and after reaching adsorptive saturation, the columns were eluted successively with 5 bed volumes (BV) of water, 5 BV of 5% (v/v) aqueous ethanol and 5 BV of 30% (v/v) aqueous ethanol at an elute flow rate of 2 BV·h−1. Total flavonoids were obtained from the 30% aqueous ethanol eluate by vacuum distillation recovery. The content of flavonoid compounds in the total flavonoids was 93.5%, which represents an improvement by about 150%. In addition, five flavonoid compounds in the product were identified as 2″-O-β-d-xylopyranosyl isoorientin, 6-C-α-l-arabipyranosyl orientin, orientin, isoorientin and vitexin by LC-ESI-MS analysis and internal standard methods. The results in this study could represent a method for the large-scale production of total flavonoids from S. austriaca.http://www.mdpi.com/1420-3049/21/6/768Scorzonera austriacaflavonoidsmacroporous resins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Xie Qiu-Shi Guo Guang-Shu Wang |
spellingShingle |
Yang Xie Qiu-Shi Guo Guang-Shu Wang Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins Molecules Scorzonera austriaca flavonoids macroporous resins |
author_facet |
Yang Xie Qiu-Shi Guo Guang-Shu Wang |
author_sort |
Yang Xie |
title |
Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins |
title_short |
Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins |
title_full |
Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins |
title_fullStr |
Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins |
title_full_unstemmed |
Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins |
title_sort |
preparative separation and purification of the total flavonoids in scorzonera austriaca with macroporous resins |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-06-01 |
description |
The use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D4020 resin columns were loaded with crude flavonoid extract solution, and after reaching adsorptive saturation, the columns were eluted successively with 5 bed volumes (BV) of water, 5 BV of 5% (v/v) aqueous ethanol and 5 BV of 30% (v/v) aqueous ethanol at an elute flow rate of 2 BV·h−1. Total flavonoids were obtained from the 30% aqueous ethanol eluate by vacuum distillation recovery. The content of flavonoid compounds in the total flavonoids was 93.5%, which represents an improvement by about 150%. In addition, five flavonoid compounds in the product were identified as 2″-O-β-d-xylopyranosyl isoorientin, 6-C-α-l-arabipyranosyl orientin, orientin, isoorientin and vitexin by LC-ESI-MS analysis and internal standard methods. The results in this study could represent a method for the large-scale production of total flavonoids from S. austriaca. |
topic |
Scorzonera austriaca flavonoids macroporous resins |
url |
http://www.mdpi.com/1420-3049/21/6/768 |
work_keys_str_mv |
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