Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life

Bacterial members of the infant gut microbiota and bacterial-derived short-chain fatty acids (SCFAs) have been shown to be protective against childhood asthma, but a role for the fungal microbiota in asthma etiology remains poorly defined. We recently reported an association between overgrowth of th...

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Main Authors:	Rozlyn CT Boutin, Charisse Petersen, Sarah E Woodward, Antonio Serapio-Palacios, Tahereh Bozorgmehr, Rachelle Loo, Alina Chalanuchpong, Mihai Cirstea, Bernard Lo, Kelsey E Huus, Weronika Barcik, Meghan B Azad, Allan B Becker, Piush J Mandhane, Theo J Moraes, Malcolm R Sears, Padmaja Subbarao, Kelly M McNagny, Stuart E Turvey, B Brett Finlay
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2021-04-01
Series:	eLife
Subjects:	pichia kudriavzevii microbiota asthma mycobiota
Online Access:	https://elifesciences.org/articles/67740

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record_format	Article
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language	English
format	Article
sources	DOAJ
author	Rozlyn CT Boutin Charisse Petersen Sarah E Woodward Antonio Serapio-Palacios Tahereh Bozorgmehr Rachelle Loo Alina Chalanuchpong Mihai Cirstea Bernard Lo Kelsey E Huus Weronika Barcik Meghan B Azad Allan B Becker Piush J Mandhane Theo J Moraes Malcolm R Sears Padmaja Subbarao Kelly M McNagny Stuart E Turvey B Brett Finlay
spellingShingle	Rozlyn CT Boutin Charisse Petersen Sarah E Woodward Antonio Serapio-Palacios Tahereh Bozorgmehr Rachelle Loo Alina Chalanuchpong Mihai Cirstea Bernard Lo Kelsey E Huus Weronika Barcik Meghan B Azad Allan B Becker Piush J Mandhane Theo J Moraes Malcolm R Sears Padmaja Subbarao Kelly M McNagny Stuart E Turvey B Brett Finlay Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life eLife pichia kudriavzevii microbiota asthma mycobiota
author_facet	Rozlyn CT Boutin Charisse Petersen Sarah E Woodward Antonio Serapio-Palacios Tahereh Bozorgmehr Rachelle Loo Alina Chalanuchpong Mihai Cirstea Bernard Lo Kelsey E Huus Weronika Barcik Meghan B Azad Allan B Becker Piush J Mandhane Theo J Moraes Malcolm R Sears Padmaja Subbarao Kelly M McNagny Stuart E Turvey B Brett Finlay
author_sort	Rozlyn CT Boutin
title	Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
title_short	Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
title_full	Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
title_fullStr	Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
title_full_unstemmed	Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
title_sort	bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2021-04-01
description	Bacterial members of the infant gut microbiota and bacterial-derived short-chain fatty acids (SCFAs) have been shown to be protective against childhood asthma, but a role for the fungal microbiota in asthma etiology remains poorly defined. We recently reported an association between overgrowth of the yeast Pichia kudriavzevii in the gut microbiota of Ecuadorian infants and increased asthma risk. In the present study, we replicated these findings in Canadian infants and investigated a causal association between early life gut fungal dysbiosis and later allergic airway disease (AAD). In a mouse model, we demonstrate that overgrowth of P. kudriavzevii within the neonatal gut exacerbates features of type-2 and -17 inflammation during AAD later in life. We further show that P. kudriavzevii growth and adherence to gut epithelial cells are altered by SCFAs. Collectively, our results underscore the potential for leveraging inter-kingdom interactions when designing putative microbiota-based asthma therapeutics.
topic	pichia kudriavzevii microbiota asthma mycobiota
url	https://elifesciences.org/articles/67740
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spelling	doaj-3528669980c6489896b4811b6c6f45262021-05-05T22:59:30ZengeLife Sciences Publications LtdeLife2050-084X2021-04-011010.7554/eLife.67740Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in lifeRozlyn CT Boutin0https://orcid.org/0000-0003-1598-0104Charisse Petersen1Sarah E Woodward2https://orcid.org/0000-0002-6688-0595Antonio Serapio-Palacios3Tahereh Bozorgmehr4Rachelle Loo5Alina Chalanuchpong6Mihai Cirstea7https://orcid.org/0000-0003-4900-6385Bernard Lo8Kelsey E Huus9Weronika Barcik10Meghan B Azad11Allan B Becker12Piush J Mandhane13Theo J Moraes14Malcolm R Sears15Padmaja Subbarao16Kelly M McNagny17https://orcid.org/0000-0003-4737-3499Stuart E Turvey18B Brett Finlay19https://orcid.org/0000-0001-5303-6128Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, CanadaMichael Smith Laboratories, University of British Columbia, Vancouver, CanadaChildren’s Hospital Research Institute of Manitoba and Department of Pediatrics and Child Health, University of Manitoba, WinnipegMB, CanadaChildren’s Hospital Research Institute of Manitoba and Department of Pediatrics and Child Health, University of Manitoba, WinnipegMB, CanadaDepartment of Pediatrics, University of Alberta, Edmonton, Canada; School of Public Health, University of Alberta, Edmonton, CanadaThe Hospital for Sick Children, Toronto, CanadaDepartment of Medicine, McMaster University, Hamilton, CanadaThe Hospital for Sick Children, Toronto, Canada; Department of Pediatrics, University of Toronto, Toronto, CanadaDepartment of Biomedical Engineering, University of British Columbia, Vancouver, Canada; Department of Medical Genetics University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Department of Pediatrics, University of British Columbia, Vancouver, CanadaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, CanadaBacterial members of the infant gut microbiota and bacterial-derived short-chain fatty acids (SCFAs) have been shown to be protective against childhood asthma, but a role for the fungal microbiota in asthma etiology remains poorly defined. We recently reported an association between overgrowth of the yeast Pichia kudriavzevii in the gut microbiota of Ecuadorian infants and increased asthma risk. In the present study, we replicated these findings in Canadian infants and investigated a causal association between early life gut fungal dysbiosis and later allergic airway disease (AAD). In a mouse model, we demonstrate that overgrowth of P. kudriavzevii within the neonatal gut exacerbates features of type-2 and -17 inflammation during AAD later in life. We further show that P. kudriavzevii growth and adherence to gut epithelial cells are altered by SCFAs. Collectively, our results underscore the potential for leveraging inter-kingdom interactions when designing putative microbiota-based asthma therapeutics.https://elifesciences.org/articles/67740pichia kudriavzeviimicrobiotaasthmamycobiota

Bacterial–fungal interactions in the neonatal gut influence asthma outcomes later in life

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