Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts
Many species of Psephellus and Centaurea genuses have pharmacological activities including antiinflamatory, antipyretic, cytotoxic etc. Psephellus pyrrhoblepharus (Boiss.) Wagenitz (Centaurea pyrrhoblephara), an endemic plant, was collected from Elazığ, Turkey. Liver cancer is affecting millions of...
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doaj-352c94fcbad042d5b2e7f026f868cf6f2020-11-24T21:27:50ZengMDPI AGProceedings2504-39002017-11-01110104710.3390/proceedings1101047proceedings1101047Potential Cytotoxic Activity of Psephellus pyrrhoblepharus ExtractsPelin Taştan0Güliz Armagan1Taner Dağcı2Bijen Kıvçak3Deparment of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir 35000, TurkeyDepartment of Biochemistry, Faculty of Pharmacy, Ege University, İzmir 35000, TurkeyDepartment of Physiology, Faculty of Medicine, Ege University, İzmir 35000, TurkeyDeparment of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir 35000, TurkeyMany species of Psephellus and Centaurea genuses have pharmacological activities including antiinflamatory, antipyretic, cytotoxic etc. Psephellus pyrrhoblepharus (Boiss.) Wagenitz (Centaurea pyrrhoblephara), an endemic plant, was collected from Elazığ, Turkey. Liver cancer is affecting millions of people all over the world. Recent days interest in use of plant-derived compounds for therapeutic purposes in cancer is increasing. So any approach to treat liver cancer is extremely valuable. We aimed to evaluate the cytotoxic potential of extracts (methanol:water 1:1, chloroform and n-hexane) of aerial parts of P. pyrrhoblepharus using human liver cancer cell (HepG2) by utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay in different concentrations (50,100,200 µg/mL) and time points (6, 12, 24 h). Regarding the cytotoxicity, cell viability was decreased following extract exposures at different time points. However, the highest cytotoxic activity was observed in 100 µg/mL concentrations of chloroform extract at 24 h. Chloroform extract of plant showed the highest cytotoxic activity in 200 µg/mL concentrations when compared to methanol:water and n-hexane extracts at 12 h. It can be suggested that used extracts of P. pyrrhoblepharus exert cytotoxic effect in HepG2 carcinoma cells in a dose- and time-dependent manner. Generally, results provide information regarding the threshold concentrations of P. pyrrhoblepharus extracts that might be used in different applications without toxicity hazards.https://www.mdpi.com/2504-3900/1/10/1047Psephellus pyrrhoblepharuscytotoxic activityHepG2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pelin Taştan Güliz Armagan Taner Dağcı Bijen Kıvçak |
spellingShingle |
Pelin Taştan Güliz Armagan Taner Dağcı Bijen Kıvçak Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts Proceedings Psephellus pyrrhoblepharus cytotoxic activity HepG2 |
author_facet |
Pelin Taştan Güliz Armagan Taner Dağcı Bijen Kıvçak |
author_sort |
Pelin Taştan |
title |
Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts |
title_short |
Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts |
title_full |
Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts |
title_fullStr |
Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts |
title_full_unstemmed |
Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts |
title_sort |
potential cytotoxic activity of psephellus pyrrhoblepharus extracts |
publisher |
MDPI AG |
series |
Proceedings |
issn |
2504-3900 |
publishDate |
2017-11-01 |
description |
Many species of Psephellus and Centaurea genuses have pharmacological activities including antiinflamatory, antipyretic, cytotoxic etc. Psephellus pyrrhoblepharus (Boiss.) Wagenitz (Centaurea pyrrhoblephara), an endemic plant, was collected from Elazığ, Turkey. Liver cancer is affecting millions of people all over the world. Recent days interest in use of plant-derived compounds for therapeutic purposes in cancer is increasing. So any approach to treat liver cancer is extremely valuable. We aimed to evaluate the cytotoxic potential of extracts (methanol:water 1:1, chloroform and n-hexane) of aerial parts of P. pyrrhoblepharus using human liver cancer cell (HepG2) by utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay in different concentrations (50,100,200 µg/mL) and time points (6, 12, 24 h). Regarding the cytotoxicity, cell viability was decreased following extract exposures at different time points. However, the highest cytotoxic activity was observed in 100 µg/mL concentrations of chloroform extract at 24 h. Chloroform extract of plant showed the highest cytotoxic activity in 200 µg/mL concentrations when compared to methanol:water and n-hexane extracts at 12 h. It can be suggested that used extracts of P. pyrrhoblepharus exert cytotoxic effect in HepG2 carcinoma cells in a dose- and time-dependent manner. Generally, results provide information regarding the threshold concentrations of P. pyrrhoblepharus extracts that might be used in different applications without toxicity hazards. |
topic |
Psephellus pyrrhoblepharus cytotoxic activity HepG2 |
url |
https://www.mdpi.com/2504-3900/1/10/1047 |
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