Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response
The mannose receptor (MAN-R)-targeted delivery system is commonly used to deliver antigens to macrophages or immature dendritic cells (DCs) to promote the efficiency of antigen presentation. To maximize the enhancement effects of chitosan (CS) and induce an efficient humoral and cellular immune resp...
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doaj-353dc1d8d3ea4005a8551681c281cffc2021-07-15T15:43:56ZengMDPI AGPolymers2073-43602021-07-01132208220810.3390/polym13132208Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune ResponseHaibo Feng0Xiaonong Yang1Linzi Zhang2Qianqian Liu3Yangyang Feng4Daiyan Wu5Yunjie Liu6Jie Yang7College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang 402460, ChinaDepartment of Veterinary Medicine, Southwest University, Rongchang 402460, ChinaThe mannose receptor (MAN-R)-targeted delivery system is commonly used to deliver antigens to macrophages or immature dendritic cells (DCs) to promote the efficiency of antigen presentation. To maximize the enhancement effects of chitosan (CS) and induce an efficient humoral and cellular immune response against an antigen, we encapsulated ovalbumin (OVA) in poly(lactic-co-glycolic acid) (PLGA) microspheres (MPs) and conjugated it with MAN-modified CS to obtain MAN-R-targeting nano-MPs (MAN-CS-OVA-PLGA-MPs). The physicochemical properties, drug loading rate, and immunomodulation activity of MAN-CS-OVA-PLGA-MPs were evaluated. In vitro, MAN-CS-OVA-PLGA-MPs (80 μg mL<sup>−1</sup>) could enhance the proliferation of DCs and increase their phagocytic efficiency. In vivo, MAN-CS-OVA-PLGA-MPs significantly increased the ratio of CD3<sup>+</sup>CD4<sup>+</sup>/CD3<sup>+</sup>CD8<sup>+</sup> T cells, increased CD80<sup>+</sup>, CD86<sup>+</sup>, and MHC II expression in DCs, and improved OVA-specific IgG, IgG1, IgG2a, and IgG2b antibodies. Moreover, MAN-CS-OVA-PLGA-MPs promoted cytokine (IFN-γ, IL-4, and IL-6) production in mice. Taken together, our results show that MAN-CS-OVA-PLGA-MPs may act by activating the T cells to initiate an immune response by promoting the maturation of dendritic cells and improving their antigen presentation efficiency. The current study provides a basis for the use of MAN-CS-OVA-PLGA-MPs as an antigen and adjuvant delivery system targeting the MAN-R on the surface of macrophages and dendritic cells.https://www.mdpi.com/2073-4360/13/13/2208mannose receptormannose-modified chitosanPLGA nanomicrospheresdelivery systemdendritic cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haibo Feng Xiaonong Yang Linzi Zhang Qianqian Liu Yangyang Feng Daiyan Wu Yunjie Liu Jie Yang |
spellingShingle |
Haibo Feng Xiaonong Yang Linzi Zhang Qianqian Liu Yangyang Feng Daiyan Wu Yunjie Liu Jie Yang Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response Polymers mannose receptor mannose-modified chitosan PLGA nanomicrospheres delivery system dendritic cells |
author_facet |
Haibo Feng Xiaonong Yang Linzi Zhang Qianqian Liu Yangyang Feng Daiyan Wu Yunjie Liu Jie Yang |
author_sort |
Haibo Feng |
title |
Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response |
title_short |
Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response |
title_full |
Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response |
title_fullStr |
Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response |
title_full_unstemmed |
Mannose-Modified Chitosan Poly(lactic-<i>co</i>-glycolic acid) Microspheres Act as a Mannose Receptor-Mediated Delivery System Enhancing the Immune Response |
title_sort |
mannose-modified chitosan poly(lactic-<i>co</i>-glycolic acid) microspheres act as a mannose receptor-mediated delivery system enhancing the immune response |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2021-07-01 |
description |
The mannose receptor (MAN-R)-targeted delivery system is commonly used to deliver antigens to macrophages or immature dendritic cells (DCs) to promote the efficiency of antigen presentation. To maximize the enhancement effects of chitosan (CS) and induce an efficient humoral and cellular immune response against an antigen, we encapsulated ovalbumin (OVA) in poly(lactic-co-glycolic acid) (PLGA) microspheres (MPs) and conjugated it with MAN-modified CS to obtain MAN-R-targeting nano-MPs (MAN-CS-OVA-PLGA-MPs). The physicochemical properties, drug loading rate, and immunomodulation activity of MAN-CS-OVA-PLGA-MPs were evaluated. In vitro, MAN-CS-OVA-PLGA-MPs (80 μg mL<sup>−1</sup>) could enhance the proliferation of DCs and increase their phagocytic efficiency. In vivo, MAN-CS-OVA-PLGA-MPs significantly increased the ratio of CD3<sup>+</sup>CD4<sup>+</sup>/CD3<sup>+</sup>CD8<sup>+</sup> T cells, increased CD80<sup>+</sup>, CD86<sup>+</sup>, and MHC II expression in DCs, and improved OVA-specific IgG, IgG1, IgG2a, and IgG2b antibodies. Moreover, MAN-CS-OVA-PLGA-MPs promoted cytokine (IFN-γ, IL-4, and IL-6) production in mice. Taken together, our results show that MAN-CS-OVA-PLGA-MPs may act by activating the T cells to initiate an immune response by promoting the maturation of dendritic cells and improving their antigen presentation efficiency. The current study provides a basis for the use of MAN-CS-OVA-PLGA-MPs as an antigen and adjuvant delivery system targeting the MAN-R on the surface of macrophages and dendritic cells. |
topic |
mannose receptor mannose-modified chitosan PLGA nanomicrospheres delivery system dendritic cells |
url |
https://www.mdpi.com/2073-4360/13/13/2208 |
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