Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications
Objective Soluble fibrin (SF) is a substantial component of plasma fibrinogen (fg), but its composition, functions, and clinical relevance remain unclear. The study aimed to evaluate the molecular composition and procoagulant function(s) of SF. Materials and Methods Cryoprecipitable, SF-r...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Georg Thieme Verlag KG
2021-07-01
|
Series: | TH Open |
Subjects: | |
Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1725976 |
id |
doaj-3586f8fcaa7141abaab9d03888eab6a8 |
---|---|
record_format |
Article |
spelling |
doaj-3586f8fcaa7141abaab9d03888eab6a82021-08-02T13:30:59ZengGeorg Thieme Verlag KGTH Open2512-94652021-07-010503e273e28510.1055/s-0041-1725976Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical ImplicationsDennis K. Galanakis0Anna Protopopova1Liudi Zhang2Kao Li3Clement Marmorat4Tomas Scheiner5Jaseung Koo6Anne G. Savitt7Miriam Rafailovich8John Weisel9Department of Pathology, Stony Brook University School of Medicine, Stony Brook, New YorkDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PennsylvaniaDepartment of Materials Science and Engineering, Stony Brook University, Stony Brook, New YorkDepartment of Materials Science and Engineering, Stony Brook University, Stony Brook, New YorkDepartment of Materials Science and Engineering, Stony Brook University, Stony Brook, New YorkDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PennsylvaniaDepartment of Materials Science and Engineering, Stony Brook University, Stony Brook, New YorkDepartment of Microbiology and Immunology, Stony Brook University School of Medicine, Stony Brook, New YorkDepartment of Materials Science and Engineering, Stony Brook University, Stony Brook, New YorkDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PennsylvaniaObjective Soluble fibrin (SF) is a substantial component of plasma fibrinogen (fg), but its composition, functions, and clinical relevance remain unclear. The study aimed to evaluate the molecular composition and procoagulant function(s) of SF. Materials and Methods Cryoprecipitable, SF-rich (FR) and cryosoluble, SF-depleted (FD) fg isolates were prepared and adsorbed on one hydrophilic and two hydrophobic surfaces and scanned by atomic force microscopy (AFM). Standard procedures were used for fibrin polymerization, crosslinking by factor XIII, electrophoresis, and platelet adhesion. Results Relative to FD fg, thrombin-induced polymerization of FR fg was accelerated and that induced by reptilase was markedly delayed, attributable to its decreased (fibrinopeptide A) FpA. FR fg adsorption to each surface yielded polymeric clusters and co-cryoprecipitable solitary monomers. Cluster components were crosslinked by factor XIII and comprised ≤21% of FR fg. In contrast to FD fg, FR fg adsorption on hydrophobic surfaces resulted in fiber generation enabled by both clusters and solitary monomers. This began with numerous short protofibrils, which following prolonged adsorption increased in number and length and culminated in surface-linked three-dimensional fiber networks that bound platelets. Conclusion The abundance of adsorbed protofibrils resulted from (1) protofibril/fg clusters whose fg was dissociated during adsorption, and (2) adsorbed des-AA monomers that attracted solution counterparts initiating protofibril assembly and elongation by their continued incorporation. The substantial presence of both components in transfused plasma and cryoprecipitate augments hemostasis by accelerating thrombin-induced fibrin polymerization and by tightly anchoring the resulting clot to the underlying wound or to other abnormal vascular surfaces.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1725976fibrin fibersfibrinogen adsorptionsoluble fibrincryoprecipitate fibrinogenprotofibril/fibrinogen clustersplatelet adhesion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dennis K. Galanakis Anna Protopopova Liudi Zhang Kao Li Clement Marmorat Tomas Scheiner Jaseung Koo Anne G. Savitt Miriam Rafailovich John Weisel |
spellingShingle |
Dennis K. Galanakis Anna Protopopova Liudi Zhang Kao Li Clement Marmorat Tomas Scheiner Jaseung Koo Anne G. Savitt Miriam Rafailovich John Weisel Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications TH Open fibrin fibers fibrinogen adsorption soluble fibrin cryoprecipitate fibrinogen protofibril/fibrinogen clusters platelet adhesion |
author_facet |
Dennis K. Galanakis Anna Protopopova Liudi Zhang Kao Li Clement Marmorat Tomas Scheiner Jaseung Koo Anne G. Savitt Miriam Rafailovich John Weisel |
author_sort |
Dennis K. Galanakis |
title |
Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications |
title_short |
Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications |
title_full |
Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications |
title_fullStr |
Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications |
title_full_unstemmed |
Fibers Generated by Plasma Des-AA Fibrin Monomers and Protofibril/Fibrinogen Clusters Bind Platelets: Clinical and Nonclinical Implications |
title_sort |
fibers generated by plasma des-aa fibrin monomers and protofibril/fibrinogen clusters bind platelets: clinical and nonclinical implications |
publisher |
Georg Thieme Verlag KG |
series |
TH Open |
issn |
2512-9465 |
publishDate |
2021-07-01 |
description |
Objective Soluble fibrin (SF) is a substantial component of plasma fibrinogen (fg), but its composition, functions, and clinical relevance remain unclear. The study aimed to evaluate the molecular composition and procoagulant function(s) of SF.
Materials and Methods Cryoprecipitable, SF-rich (FR) and cryosoluble, SF-depleted (FD) fg isolates were prepared and adsorbed on one hydrophilic and two hydrophobic surfaces and scanned by atomic force microscopy (AFM). Standard procedures were used for fibrin polymerization, crosslinking by factor XIII, electrophoresis, and platelet adhesion.
Results Relative to FD fg, thrombin-induced polymerization of FR fg was accelerated and that induced by reptilase was markedly delayed, attributable to its decreased (fibrinopeptide A) FpA. FR fg adsorption to each surface yielded polymeric clusters and co-cryoprecipitable solitary monomers. Cluster components were crosslinked by factor XIII and comprised ≤21% of FR fg. In contrast to FD fg, FR fg adsorption on hydrophobic surfaces resulted in fiber generation enabled by both clusters and solitary monomers. This began with numerous short protofibrils, which following prolonged adsorption increased in number and length and culminated in surface-linked three-dimensional fiber networks that bound platelets.
Conclusion The abundance of adsorbed protofibrils resulted from (1) protofibril/fg clusters whose fg was dissociated during adsorption, and (2) adsorbed des-AA monomers that attracted solution counterparts initiating protofibril assembly and elongation by their continued incorporation. The substantial presence of both components in transfused plasma and cryoprecipitate augments hemostasis by accelerating thrombin-induced fibrin polymerization and by tightly anchoring the resulting clot to the underlying wound or to other abnormal vascular surfaces. |
topic |
fibrin fibers fibrinogen adsorption soluble fibrin cryoprecipitate fibrinogen protofibril/fibrinogen clusters platelet adhesion |
url |
http://www.thieme-connect.de/DOI/DOI?10.1055/s-0041-1725976 |
work_keys_str_mv |
AT denniskgalanakis fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT annaprotopopova fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT liudizhang fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT kaoli fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT clementmarmorat fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT tomasscheiner fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT jaseungkoo fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT annegsavitt fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT miriamrafailovich fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications AT johnweisel fibersgeneratedbyplasmadesaafibrinmonomersandprotofibrilfibrinogenclustersbindplateletsclinicalandnonclinicalimplications |
_version_ |
1721231942869843968 |