Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3
Abstract Background Synthetic biomaterials assist in modulating the vascular response in an injured bone by serving as delivery vehicles of pro-angiogenic molecules to the site of injury or by serving as mimetic platforms which offer support to cell growth and proliferation. Methods This study appli...
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doaj-358ad64381cf411782075d19ce14166f2021-02-14T12:25:18ZengBMCJournal of Biological Engineering1754-16112021-02-011511810.1186/s13036-021-00258-5Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3Wei He0Daixu Wei1Jun Zhang2Xiaonan Huang3Da He4Bo Liu5Qilong Wang6Mingming Liu7Ling Liu8Yajun Liu9Wei Tian10Department of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Biomaterials and Microorganisms, Northwest UniversityDepartment of Spine Surgery, Zhejiang Provincial People’s Hospital, Hangzhou Medical College People’s HospitalDepartment of Chemistry, Capital Normal UniversityDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Gynaecology and Obstetrics, Third Medical Center of Chinese PLA General HospitalDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityDepartment of Spine Surgery, Beijing JiShuiTan Hospital, 4th Medical College of Peking UniversityAbstract Background Synthetic biomaterials assist in modulating the vascular response in an injured bone by serving as delivery vehicles of pro-angiogenic molecules to the site of injury or by serving as mimetic platforms which offer support to cell growth and proliferation. Methods This study applied natural phospholipid modified protein technologies together with low temperature three-dimensional printing technology to develop a new model of three-dimensional artificial bone scaffold for potential use in repairing body injuries. The focus was to create a porous structure (PS) scaffold of two components, Bone Morphogenetic Protein-2 and Human Beta Defensin-3 (BMP2 and hBD3), which can synchronously realize directional bone induction, angiogenesis and postoperative antibacterial effects. BMP2 induces osteogenesis, whereas hBD3 is antibacterial. Results Our data showed that in the BMP2-hBD3-PS or hBD3-PS scaffolds, BMP2 had a slow-release rate of about 40% in 30 days, ensuring that BMP2 could penetrate into stem cells for osteogenic differentiation for a long time. The scaffolds promoted cell growth when in combination with BMP2, thus showing its importance in promoting cell growth. Alkaline Phosphatase (ALP) staining showed that the ALP content of BMP2-hBD3-PS and BMP2-PS had a significant increase in samples that contained BMP2, thus showing that these scaffolds promoted osteogenic differentiation. In all the constructs that had hBD3, they displayed antibacterial properties with hBD3, having a slow release of about 35% in 30 days, thus ensuring they provided protection. Conclusion Based on this study, the 3D printed BMP2 scaffolds show a great potential for the development of biodegradable bone implants. Level of evidence Level II, experimental comparative design.https://doi.org/10.1186/s13036-021-00258-5Artificial bone scaffoldBone repairPhospholipid modified proteinBone injury |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei He Daixu Wei Jun Zhang Xiaonan Huang Da He Bo Liu Qilong Wang Mingming Liu Ling Liu Yajun Liu Wei Tian |
spellingShingle |
Wei He Daixu Wei Jun Zhang Xiaonan Huang Da He Bo Liu Qilong Wang Mingming Liu Ling Liu Yajun Liu Wei Tian Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 Journal of Biological Engineering Artificial bone scaffold Bone repair Phospholipid modified protein Bone injury |
author_facet |
Wei He Daixu Wei Jun Zhang Xiaonan Huang Da He Bo Liu Qilong Wang Mingming Liu Ling Liu Yajun Liu Wei Tian |
author_sort |
Wei He |
title |
Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 |
title_short |
Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 |
title_full |
Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 |
title_fullStr |
Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 |
title_full_unstemmed |
Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3 |
title_sort |
novel bone repairing scaffold consisting of bone morphogenetic protein-2 and human beta defensin-3 |
publisher |
BMC |
series |
Journal of Biological Engineering |
issn |
1754-1611 |
publishDate |
2021-02-01 |
description |
Abstract Background Synthetic biomaterials assist in modulating the vascular response in an injured bone by serving as delivery vehicles of pro-angiogenic molecules to the site of injury or by serving as mimetic platforms which offer support to cell growth and proliferation. Methods This study applied natural phospholipid modified protein technologies together with low temperature three-dimensional printing technology to develop a new model of three-dimensional artificial bone scaffold for potential use in repairing body injuries. The focus was to create a porous structure (PS) scaffold of two components, Bone Morphogenetic Protein-2 and Human Beta Defensin-3 (BMP2 and hBD3), which can synchronously realize directional bone induction, angiogenesis and postoperative antibacterial effects. BMP2 induces osteogenesis, whereas hBD3 is antibacterial. Results Our data showed that in the BMP2-hBD3-PS or hBD3-PS scaffolds, BMP2 had a slow-release rate of about 40% in 30 days, ensuring that BMP2 could penetrate into stem cells for osteogenic differentiation for a long time. The scaffolds promoted cell growth when in combination with BMP2, thus showing its importance in promoting cell growth. Alkaline Phosphatase (ALP) staining showed that the ALP content of BMP2-hBD3-PS and BMP2-PS had a significant increase in samples that contained BMP2, thus showing that these scaffolds promoted osteogenic differentiation. In all the constructs that had hBD3, they displayed antibacterial properties with hBD3, having a slow release of about 35% in 30 days, thus ensuring they provided protection. Conclusion Based on this study, the 3D printed BMP2 scaffolds show a great potential for the development of biodegradable bone implants. Level of evidence Level II, experimental comparative design. |
topic |
Artificial bone scaffold Bone repair Phospholipid modified protein Bone injury |
url |
https://doi.org/10.1186/s13036-021-00258-5 |
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