Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae)
Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucros...
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Associação Brasileira de Divulgação Científica
1999-01-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400010 |
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doaj-358f442ee5d54979b44de007d42f40062020-11-24T21:15:27ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73101999-01-01324435442Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae)Itaya N.M.Figueiredo-Ribeiro R.C.L.Buckeridge M.S.Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn), when both (SST and FFT) activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecular mass fructans from sucrose by enzymatic preparations from tuberous roots collected in summer showed that long incubations of up to 288 h produced consistently longer polymers which resembled those found in vivo with respect to chromatographic profiles.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400010inulinfructosyltransferasefructan biosynthesisViguiera discolorAsteraceae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Itaya N.M. Figueiredo-Ribeiro R.C.L. Buckeridge M.S. |
spellingShingle |
Itaya N.M. Figueiredo-Ribeiro R.C.L. Buckeridge M.S. Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) Brazilian Journal of Medical and Biological Research inulin fructosyltransferase fructan biosynthesis Viguiera discolor Asteraceae |
author_facet |
Itaya N.M. Figueiredo-Ribeiro R.C.L. Buckeridge M.S. |
author_sort |
Itaya N.M. |
title |
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) |
title_short |
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) |
title_full |
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) |
title_fullStr |
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) |
title_full_unstemmed |
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae) |
title_sort |
synthesis of fructans by fructosyltransferase from the tuberous roots of viguiera discolor (asteraceae) |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 0034-7310 |
publishDate |
1999-01-01 |
description |
Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn), when both (SST and FFT) activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecular mass fructans from sucrose by enzymatic preparations from tuberous roots collected in summer showed that long incubations of up to 288 h produced consistently longer polymers which resembled those found in vivo with respect to chromatographic profiles. |
topic |
inulin fructosyltransferase fructan biosynthesis Viguiera discolor Asteraceae |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400010 |
work_keys_str_mv |
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