Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats
Obstructive jaundice (OJ) can be defined as cessation of bile flow into the small intestine due to benign or malignant changes. Nesfatin-1, recently discovered anorexigenic peptide derived from nucleobindin-2 in hypothalamic nuclei, was shown to have anti-inflammatory and antiapoptotic effects. Thi...
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doaj-359fed00bb8a4b2f897451c564750d382020-11-24T22:08:16ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBosnian Journal of Basic Medical Sciences1512-86011840-48122016-11-0116410.17305/bjbms.2016.1465126Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in ratsAli Solmaz0Osman Bilgin Gülçiçek1Candaş Erçetin2Hakan Yiğitbaş3Erkan Yavuz4Sinan Arıcı5Can Erzik6Oğuzhan Zengi7Pelin Demirtürk8Atilla Çelik9Fatih Çelebi10General Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyDepartment of Medical Biology, Marmara University Faculty of Medicine, Istanbul, TurkeyBiochemistry Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyPathology Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, TurkeyGeneral Surgery Clinic, Bağcılar Training and Research Hospital, Istanbul, Turkey Obstructive jaundice (OJ) can be defined as cessation of bile flow into the small intestine due to benign or malignant changes. Nesfatin-1, recently discovered anorexigenic peptide derived from nucleobindin-2 in hypothalamic nuclei, was shown to have anti-inflammatory and antiapoptotic effects. This study is aimed to investigate the therapeutic effects of nesfatin-1 on OJ in rats. Twenty-four adult male Wistar-Hannover rats were randomly assigned to three groups: sham (n = 8), control (n = 8), and nesfatin (n = 8). After bile duct ligation, the study groups were treated with saline or nesfatin-1, for 10 days. Afterward, blood and liver tissue samples were obtained for biochemical analyses, measurement of cytokines, determination of the oxidative DNA damage, DNA fragmentation, and histopathologic analyses. Alanine aminotransferase and gamma-glutamyl transferase levels were decreased after the nesfatin treatment; however, these drops were statistically non-significant compared to control group (p = 0.345, p = 0.114). Malondialdehyde levels decreased significantly in nesfatin group compared to control group (p = 0.032). Decreases in interleukin-6 and tumor necrosis factor-α levels from the liver tissue samples were not statistically significant in nesfatin group compared to control group. The level of oxidative DNA damage was lower in nesfatin group, however this result was not statistically significant (p = 0.75). DNA fragmentation results of all groups were similar. Histopathological examination revealed that there was less neutrophil infiltration, edema, bile duct proliferation, hepatocyte necrosis, basement membrane damage, and parenchymal necrosis in nesfatin compared to control group. The nesfatin-1 treatment could alleviate cholestatic liver damage caused by OJ due to its anti-inflammatory and antioxidant effects. https://bjbms.org/ojs/index.php/bjbms/article/view/1465Obstructive jaundiceoxidative stresshepatic damagecholestasisDNA fragmentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Solmaz Osman Bilgin Gülçiçek Candaş Erçetin Hakan Yiğitbaş Erkan Yavuz Sinan Arıcı Can Erzik Oğuzhan Zengi Pelin Demirtürk Atilla Çelik Fatih Çelebi |
spellingShingle |
Ali Solmaz Osman Bilgin Gülçiçek Candaş Erçetin Hakan Yiğitbaş Erkan Yavuz Sinan Arıcı Can Erzik Oğuzhan Zengi Pelin Demirtürk Atilla Çelik Fatih Çelebi Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats Bosnian Journal of Basic Medical Sciences Obstructive jaundice oxidative stress hepatic damage cholestasis DNA fragmentation |
author_facet |
Ali Solmaz Osman Bilgin Gülçiçek Candaş Erçetin Hakan Yiğitbaş Erkan Yavuz Sinan Arıcı Can Erzik Oğuzhan Zengi Pelin Demirtürk Atilla Çelik Fatih Çelebi |
author_sort |
Ali Solmaz |
title |
Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
title_short |
Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
title_full |
Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
title_fullStr |
Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
title_full_unstemmed |
Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
title_sort |
nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats |
publisher |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
series |
Bosnian Journal of Basic Medical Sciences |
issn |
1512-8601 1840-4812 |
publishDate |
2016-11-01 |
description |
Obstructive jaundice (OJ) can be defined as cessation of bile flow into the small intestine due to benign or malignant changes. Nesfatin-1, recently discovered anorexigenic peptide derived from nucleobindin-2 in hypothalamic nuclei, was shown to have anti-inflammatory and antiapoptotic effects. This study is aimed to investigate the therapeutic effects of nesfatin-1 on OJ in rats. Twenty-four adult male Wistar-Hannover rats were randomly assigned to three groups: sham (n = 8), control (n = 8), and nesfatin (n = 8). After bile duct ligation, the study groups were treated with saline or nesfatin-1, for 10 days. Afterward, blood and liver tissue samples were obtained for biochemical analyses, measurement of cytokines, determination of the oxidative DNA damage, DNA fragmentation, and histopathologic analyses. Alanine aminotransferase and gamma-glutamyl transferase levels were decreased after the nesfatin treatment; however, these drops were statistically non-significant compared to control group (p = 0.345, p = 0.114). Malondialdehyde levels decreased significantly in nesfatin group compared to control group (p = 0.032). Decreases in interleukin-6 and tumor necrosis factor-α levels from the liver tissue samples were not statistically significant in nesfatin group compared to control group. The level of oxidative DNA damage was lower in nesfatin group, however this result was not statistically significant (p = 0.75). DNA fragmentation results of all groups were similar. Histopathological examination revealed that there was less neutrophil infiltration, edema, bile duct proliferation, hepatocyte necrosis, basement membrane damage, and parenchymal necrosis in nesfatin compared to control group. The nesfatin-1 treatment could alleviate cholestatic liver damage caused by OJ due to its anti-inflammatory and antioxidant effects.
|
topic |
Obstructive jaundice oxidative stress hepatic damage cholestasis DNA fragmentation |
url |
https://bjbms.org/ojs/index.php/bjbms/article/view/1465 |
work_keys_str_mv |
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