Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
Abstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the...
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doaj-35af696ad3974bba94f9d156acbd48d62021-07-14T06:50:33ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762021-05-013531288129610.1111/jvim.16141Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathiesAnita Manz0Karin Allenspach1Stefan Kummer2Barbara Richter3Ingrid Walter4Sabine Macho‐Maschler5Alexander Tichy6Iwan A Burgener7Nicole Luckschander‐Zeller8Internal Medicine, Clinic for Small Animals, Department for Companion Animals and Horses University of Veterinary Medicine Vienna AustriaDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment for Pathobiology University of Veterinary Medicine Vienna AustriaInstitute of Pathology University of Veterinary Medicine Vienna AustriaVetCore Facility for Research University of Veterinary Medicine Vienna AustriaInstitute of Animal Breeding and Genetics University of Veterinary Medicine Vienna AustriaDepartment of Biomedical Sciences University of Veterinary Medicine Vienna AustriaInternal Medicine, Clinic for Small Animals, Department for Companion Animals and Horses University of Veterinary Medicine Vienna AustriaInternal Medicine, Clinic for Small Animals, Department for Companion Animals and Horses University of Veterinary Medicine Vienna AustriaAbstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring. Animals Twenty‐eight canine CIE patients grouped into food‐responsive enteropathy (FRE; 9), steroid‐responsive enteropathy (SRE; 10), and protein‐losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO). Methods Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples. Results Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02). Conclusions and Clinical Importance pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE.https://doi.org/10.1111/jvim.16141caninechronic inflammatory enteropathyIBDSTAT3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller |
spellingShingle |
Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies Journal of Veterinary Internal Medicine canine chronic inflammatory enteropathy IBD STAT3 |
author_facet |
Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller |
author_sort |
Anita Manz |
title |
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
title_short |
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
title_full |
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
title_fullStr |
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
title_full_unstemmed |
Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
title_sort |
upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies |
publisher |
Wiley |
series |
Journal of Veterinary Internal Medicine |
issn |
0891-6640 1939-1676 |
publishDate |
2021-05-01 |
description |
Abstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring. Animals Twenty‐eight canine CIE patients grouped into food‐responsive enteropathy (FRE; 9), steroid‐responsive enteropathy (SRE; 10), and protein‐losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO). Methods Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples. Results Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02). Conclusions and Clinical Importance pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE. |
topic |
canine chronic inflammatory enteropathy IBD STAT3 |
url |
https://doi.org/10.1111/jvim.16141 |
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