Matrin 3 binds and stabilizes mRNA.
Matrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand br...
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doaj-35b84ac849f44fbdabeb72c4140aa5f42020-11-25T02:15:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2388210.1371/journal.pone.0023882Matrin 3 binds and stabilizes mRNA.Maayan SaltonRan ElkonTatiana BorodinaAleksey DavydovMarie-Laure YaspoEran HalperinYosef ShilohMatrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand breaks in the DNA. Here, we show that MATR3 interacts in an RNA-dependent manner with several proteins with established roles in RNA processing, and maintains its interaction with RNA via its RRM2 domain. Deep sequencing of the bound RNA (RIP-seq) identified several small noncoding RNA species. Using microarray analysis to explore MATR3's role in transcription, we identified 77 transcripts whose amounts depended on the presence of MATR3. We validated this finding with nine transcripts which were also bound to the MATR3 complex. Finally, we demonstrated the importance of MATR3 for maintaining the stability of several of these mRNA species and conclude that it has a role in mRNA stabilization. The data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts.http://europepmc.org/articles/PMC3157474?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maayan Salton Ran Elkon Tatiana Borodina Aleksey Davydov Marie-Laure Yaspo Eran Halperin Yosef Shiloh |
spellingShingle |
Maayan Salton Ran Elkon Tatiana Borodina Aleksey Davydov Marie-Laure Yaspo Eran Halperin Yosef Shiloh Matrin 3 binds and stabilizes mRNA. PLoS ONE |
author_facet |
Maayan Salton Ran Elkon Tatiana Borodina Aleksey Davydov Marie-Laure Yaspo Eran Halperin Yosef Shiloh |
author_sort |
Maayan Salton |
title |
Matrin 3 binds and stabilizes mRNA. |
title_short |
Matrin 3 binds and stabilizes mRNA. |
title_full |
Matrin 3 binds and stabilizes mRNA. |
title_fullStr |
Matrin 3 binds and stabilizes mRNA. |
title_full_unstemmed |
Matrin 3 binds and stabilizes mRNA. |
title_sort |
matrin 3 binds and stabilizes mrna. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Matrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand breaks in the DNA. Here, we show that MATR3 interacts in an RNA-dependent manner with several proteins with established roles in RNA processing, and maintains its interaction with RNA via its RRM2 domain. Deep sequencing of the bound RNA (RIP-seq) identified several small noncoding RNA species. Using microarray analysis to explore MATR3's role in transcription, we identified 77 transcripts whose amounts depended on the presence of MATR3. We validated this finding with nine transcripts which were also bound to the MATR3 complex. Finally, we demonstrated the importance of MATR3 for maintaining the stability of several of these mRNA species and conclude that it has a role in mRNA stabilization. The data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts. |
url |
http://europepmc.org/articles/PMC3157474?pdf=render |
work_keys_str_mv |
AT maayansalton matrin3bindsandstabilizesmrna AT ranelkon matrin3bindsandstabilizesmrna AT tatianaborodina matrin3bindsandstabilizesmrna AT alekseydavydov matrin3bindsandstabilizesmrna AT marielaureyaspo matrin3bindsandstabilizesmrna AT eranhalperin matrin3bindsandstabilizesmrna AT yosefshiloh matrin3bindsandstabilizesmrna |
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