Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
Abstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional r...
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doaj-35cd4b47215748fbab275edf21118cab2021-02-21T12:31:30ZengNature Publishing GroupScientific Reports2045-23222021-02-011111810.1038/s41598-021-83047-wPlasma microRNA signature associated with retinopathy in patients with type 2 diabetesDonato Santovito0Lisa Toto1Velia De Nardis2Pamela Marcantonio3Rossella D’Aloisio4Alessandra Mastropasqua5Domenico De Cesare6Marco Bucci7Camilla Paganelli8Lucia Natarelli9Christian Weber10Agostino Consoli11Rodolfo Mastropasqua12Francesco Cipollone13Department of Medicine and Aging Science, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityOphthalmology Clinic, “G. d’Annunzio” UniversityOphthalmology Clinic, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityGerman Centre for Cardiovascular Research (DZHK), Partner Site Munich Heart AllianceGerman Centre for Cardiovascular Research (DZHK), Partner Site Munich Heart AllianceDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityInstitute of Ophthalmology, University of Modena and Reggio EmiliaDepartment of Medicine and Aging Science, “G. d’Annunzio” UniversityAbstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression which are released in the bloodstream and may serve as biomarkers. Little is known on circulating miRNAs in patients with type 2 diabetes (T2DM) and DR. Here we show that DR is associated with higher circulating miR-25-3p (P = 0.004) and miR-320b (P = 0.011) and lower levels of miR-495-3p (P < 0.001) in a cohort of patients with T2DM with DR (n = 20), compared with diabetic subjects without DR (n = 10) and healthy individuals (n = 10). These associations persisted significant after adjustment for age, gender, and HbA1c. The circulating levels of these miRNAs correlated with severity of the disease and their concomitant evaluation showed high accuracy for identifying DR (AUROC = 0.93; P < 0.001). Gene ontology analysis of validated targets revealed enrichment in pathways such as regulation of metabolic process (P = 1.5 × 10–20), of cell response to stress (P = 1.9 × 10–14), and development of blood vessels (P = 2.7 × 10–14). Pending external validation, we anticipate that these miRNAs may serve as putative disease biomarkers and highlight novel molecular targets for improving care of patients with diabetic retinopathy.https://doi.org/10.1038/s41598-021-83047-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone |
spellingShingle |
Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes Scientific Reports |
author_facet |
Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone |
author_sort |
Donato Santovito |
title |
Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes |
title_short |
Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes |
title_full |
Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes |
title_fullStr |
Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes |
title_full_unstemmed |
Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes |
title_sort |
plasma microrna signature associated with retinopathy in patients with type 2 diabetes |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-02-01 |
description |
Abstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression which are released in the bloodstream and may serve as biomarkers. Little is known on circulating miRNAs in patients with type 2 diabetes (T2DM) and DR. Here we show that DR is associated with higher circulating miR-25-3p (P = 0.004) and miR-320b (P = 0.011) and lower levels of miR-495-3p (P < 0.001) in a cohort of patients with T2DM with DR (n = 20), compared with diabetic subjects without DR (n = 10) and healthy individuals (n = 10). These associations persisted significant after adjustment for age, gender, and HbA1c. The circulating levels of these miRNAs correlated with severity of the disease and their concomitant evaluation showed high accuracy for identifying DR (AUROC = 0.93; P < 0.001). Gene ontology analysis of validated targets revealed enrichment in pathways such as regulation of metabolic process (P = 1.5 × 10–20), of cell response to stress (P = 1.9 × 10–14), and development of blood vessels (P = 2.7 × 10–14). Pending external validation, we anticipate that these miRNAs may serve as putative disease biomarkers and highlight novel molecular targets for improving care of patients with diabetic retinopathy. |
url |
https://doi.org/10.1038/s41598-021-83047-w |
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