Numerical study of cell cryo-preservation: a network model of intracellular ice formation.
In this study, a new intracellular ice formation network model, coupled with an improved cell dehydration model has been developed. The non-uniform dehydration of the cell during freezing is simulated with moving boundary condition. Internal cell structures like cell nucleus are taken into considera...
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2013-01-01
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doaj-35d56c1c277b4412996981e87994f7ff2020-11-25T01:15:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5834310.1371/journal.pone.0058343Numerical study of cell cryo-preservation: a network model of intracellular ice formation.Wei LiGeer YangAili ZhangLisa X XuIn this study, a new intracellular ice formation network model, coupled with an improved cell dehydration model has been developed. The non-uniform dehydration of the cell during freezing is simulated with moving boundary condition. Internal cell structures like cell nucleus are taken into consideration. The IIF network model is developed from classic diffusion limited IIF model in order to simulate spatial ice growth pattern inside cells. Simulation results suggest that cell nuclear plays a significant role in cryo-dehydration and would affect water/CPA concentration gradient inside the cell. At the same time, the ice growth pattern of exogenous IIF hypothesis is examined in the model. It is consistent with our previous experiments, in which we witnessed the intracellular ice first grown into the nucleus before spreading to the whole intercellular space. According to this model, the water concentration difference between nucleus and cytoplasm during cryo-dehydration could partly explain why ice crystal in the nucleus grows faster. However, it is not the dominate factor. Higher diffusion coefficient in cell nucleus might play a more important role in the phenomenon.http://europepmc.org/articles/PMC3603964?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Li Geer Yang Aili Zhang Lisa X Xu |
spellingShingle |
Wei Li Geer Yang Aili Zhang Lisa X Xu Numerical study of cell cryo-preservation: a network model of intracellular ice formation. PLoS ONE |
author_facet |
Wei Li Geer Yang Aili Zhang Lisa X Xu |
author_sort |
Wei Li |
title |
Numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
title_short |
Numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
title_full |
Numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
title_fullStr |
Numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
title_full_unstemmed |
Numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
title_sort |
numerical study of cell cryo-preservation: a network model of intracellular ice formation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
In this study, a new intracellular ice formation network model, coupled with an improved cell dehydration model has been developed. The non-uniform dehydration of the cell during freezing is simulated with moving boundary condition. Internal cell structures like cell nucleus are taken into consideration. The IIF network model is developed from classic diffusion limited IIF model in order to simulate spatial ice growth pattern inside cells. Simulation results suggest that cell nuclear plays a significant role in cryo-dehydration and would affect water/CPA concentration gradient inside the cell. At the same time, the ice growth pattern of exogenous IIF hypothesis is examined in the model. It is consistent with our previous experiments, in which we witnessed the intracellular ice first grown into the nucleus before spreading to the whole intercellular space. According to this model, the water concentration difference between nucleus and cytoplasm during cryo-dehydration could partly explain why ice crystal in the nucleus grows faster. However, it is not the dominate factor. Higher diffusion coefficient in cell nucleus might play a more important role in the phenomenon. |
url |
http://europepmc.org/articles/PMC3603964?pdf=render |
work_keys_str_mv |
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