Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the inter...
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doaj-35da9683d4024340ae3e7e4d95f21b532020-11-24T21:15:21ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-12-011812262310.3390/ijms18122623ijms18122623Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary StructuresJohn Oliver Nealon0Limcy Seby Philomina1Liam James McGuffin2School of Biological Sciences, University of Reading, Reading RG6 6AS, UKSchool of Biological Sciences, University of Reading, Reading RG6 6AS, UKSchool of Biological Sciences, University of Reading, Reading RG6 6AS, UKThe elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the interacting partner proteins of predicted protein structures is very time-consuming when using in vitro methods, this can be somewhat alleviated by use of predictive methods. However, developing reliably accurate predictive tools has proved to be difficult. We review the current state of the art in predictive protein interaction software and discuss the problem of scoring and therefore ranking predictions. Current community-based predictive exercises are discussed in relation to the growth of protein interaction prediction as an area within these exercises. We suggest a fusion of experimental and predictive methods that make use of sparse experimental data to determine higher resolution predicted protein interactions as being necessary to drive forward development.https://www.mdpi.com/1422-0067/18/12/2623proteininteractionpredictionhomologydockingquality assessmentexperimental |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
John Oliver Nealon Limcy Seby Philomina Liam James McGuffin |
spellingShingle |
John Oliver Nealon Limcy Seby Philomina Liam James McGuffin Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures International Journal of Molecular Sciences protein interaction prediction homology docking quality assessment experimental |
author_facet |
John Oliver Nealon Limcy Seby Philomina Liam James McGuffin |
author_sort |
John Oliver Nealon |
title |
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures |
title_short |
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures |
title_full |
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures |
title_fullStr |
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures |
title_full_unstemmed |
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures |
title_sort |
predictive and experimental approaches for elucidating protein–protein interactions and quaternary structures |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-12-01 |
description |
The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the interacting partner proteins of predicted protein structures is very time-consuming when using in vitro methods, this can be somewhat alleviated by use of predictive methods. However, developing reliably accurate predictive tools has proved to be difficult. We review the current state of the art in predictive protein interaction software and discuss the problem of scoring and therefore ranking predictions. Current community-based predictive exercises are discussed in relation to the growth of protein interaction prediction as an area within these exercises. We suggest a fusion of experimental and predictive methods that make use of sparse experimental data to determine higher resolution predicted protein interactions as being necessary to drive forward development. |
topic |
protein interaction prediction homology docking quality assessment experimental |
url |
https://www.mdpi.com/1422-0067/18/12/2623 |
work_keys_str_mv |
AT johnolivernealon predictiveandexperimentalapproachesforelucidatingproteinproteininteractionsandquaternarystructures AT limcysebyphilomina predictiveandexperimentalapproachesforelucidatingproteinproteininteractionsandquaternarystructures AT liamjamesmcguffin predictiveandexperimentalapproachesforelucidatingproteinproteininteractionsandquaternarystructures |
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1716745667253108736 |