Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats
Aims: The daily activity of osteoarthritis (OA) patients is limited by chronic pain and central sensitization. Although non-steroidal anti-inflammatory drugs (NSAIDs) and acetaminophen are the first-line drugs for the treatment of OA-related pain, their efficacy on central sensitization remains uncl...
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doaj-35e080b0843d4176be67c1a87d36a8b82020-11-25T03:29:33ZengElsevierHeliyon2405-84402020-05-0165e03963Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model ratsMizuho Niibori0Yasushi Kudo1Takuya Hayakawa2Keiko Ikoma-Seki3Ryosuke Kawamata4Atsushi Sato5Kazue Mizumura6Pharmaceutical Research Laboratories, Research & Development Headquarters, Lion Corporation, Japan; Corresponding author.Pharmaceutical Research Laboratories, Research & Development Headquarters, Lion Corporation, JapanPharmaceutical Research Laboratories, Research & Development Headquarters, Lion Corporation, JapanPharmaceutical Research Laboratories, Research & Development Headquarters, Lion Corporation, JapanAdvanced Analytical Science Laboratories, Research & Development Headquarters, Lion Corporation, JapanAdvanced Analytical Science Laboratories, Research & Development Headquarters, Lion Corporation, JapanDepartment of Physiology, Nihon University School of Dentistry, Japan; College of Life and Health Sciences, Chubu University, JapanAims: The daily activity of osteoarthritis (OA) patients is limited by chronic pain and central sensitization. Although non-steroidal anti-inflammatory drugs (NSAIDs) and acetaminophen are the first-line drugs for the treatment of OA-related pain, their efficacy on central sensitization remains unclear. In the present study, we evaluated the effect of acetylsalicylic acid (ASA, Aspirin) using an OA model induced by monosodium iodoacetate (MIA), which has a similar disease progression to human OA. Main methods: Secondary hyperalgesia was assessed at the plantar surface of the hind paw by Von Frey test. We evaluated the expression of acid-sensing ion channel 3 (ASIC3) in dorsal root ganglia and that of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the spinal cord, which may cause secondary hyperalgesia in OA, by immunohistochemical analysis and real-time qPCR. Key findings: The administration of ASA attenuated secondary hyperalgesia at 1–3 weeks after MIA, while celecoxib, a selective cyclooxygenase (COX)-2 inhibitor, failed to attenuate secondary hyperalgesia at week 2 after MIA injection, suggesting that ASA exerts its analgesic effect through a COX-2-independent pathway. Immunohistochemical analysis of the dorsal root ganglia indicated that ASA reduced the expression of ASIC3 during OA progression. Expression of TNF-α mRNA, but not IL-1β mRNA, in the spinal cord following MIA injection was suppressed by ASA administration. Significance: These findings suggest that ASA may have the ability to attenuate secondary hyperalgesia through suppression of ASIC3 and/or TNF-α expression. ASA is therefore a clinically useful analgesic drug for treatment of secondary hyperalgesia in OA.http://www.sciencedirect.com/science/article/pii/S2405844020308082OsteoarthritisAcetylsalicylic acidSecondary hyperalgesiaMonosodium iodoacetateRat modelImmunology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mizuho Niibori Yasushi Kudo Takuya Hayakawa Keiko Ikoma-Seki Ryosuke Kawamata Atsushi Sato Kazue Mizumura |
spellingShingle |
Mizuho Niibori Yasushi Kudo Takuya Hayakawa Keiko Ikoma-Seki Ryosuke Kawamata Atsushi Sato Kazue Mizumura Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats Heliyon Osteoarthritis Acetylsalicylic acid Secondary hyperalgesia Monosodium iodoacetate Rat model Immunology |
author_facet |
Mizuho Niibori Yasushi Kudo Takuya Hayakawa Keiko Ikoma-Seki Ryosuke Kawamata Atsushi Sato Kazue Mizumura |
author_sort |
Mizuho Niibori |
title |
Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
title_short |
Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
title_full |
Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
title_fullStr |
Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
title_full_unstemmed |
Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
title_sort |
mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2020-05-01 |
description |
Aims: The daily activity of osteoarthritis (OA) patients is limited by chronic pain and central sensitization. Although non-steroidal anti-inflammatory drugs (NSAIDs) and acetaminophen are the first-line drugs for the treatment of OA-related pain, their efficacy on central sensitization remains unclear. In the present study, we evaluated the effect of acetylsalicylic acid (ASA, Aspirin) using an OA model induced by monosodium iodoacetate (MIA), which has a similar disease progression to human OA. Main methods: Secondary hyperalgesia was assessed at the plantar surface of the hind paw by Von Frey test. We evaluated the expression of acid-sensing ion channel 3 (ASIC3) in dorsal root ganglia and that of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the spinal cord, which may cause secondary hyperalgesia in OA, by immunohistochemical analysis and real-time qPCR. Key findings: The administration of ASA attenuated secondary hyperalgesia at 1–3 weeks after MIA, while celecoxib, a selective cyclooxygenase (COX)-2 inhibitor, failed to attenuate secondary hyperalgesia at week 2 after MIA injection, suggesting that ASA exerts its analgesic effect through a COX-2-independent pathway. Immunohistochemical analysis of the dorsal root ganglia indicated that ASA reduced the expression of ASIC3 during OA progression. Expression of TNF-α mRNA, but not IL-1β mRNA, in the spinal cord following MIA injection was suppressed by ASA administration. Significance: These findings suggest that ASA may have the ability to attenuate secondary hyperalgesia through suppression of ASIC3 and/or TNF-α expression. ASA is therefore a clinically useful analgesic drug for treatment of secondary hyperalgesia in OA. |
topic |
Osteoarthritis Acetylsalicylic acid Secondary hyperalgesia Monosodium iodoacetate Rat model Immunology |
url |
http://www.sciencedirect.com/science/article/pii/S2405844020308082 |
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